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Regulated expression of TAF1 in 1-cell mouse embryos

Published online by Cambridge University Press:  01 August 2006

Kai Wang ,
Feng Sun  and
Hui Z. Sheng
Kai Wang
Affiliation:
Center for Developmental Biology, Xinhua Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, P. R. China.
Feng Sun
Affiliation:
Laboratory of Stem Cell Biology, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, P. R. China.
Hui Z. Sheng*
Affiliation:
Laboratory of Stem Cell Biology, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, P. R. China. Center for Developmental Biology, Xinhua Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, P. R. China.
*
All correspondence to: Hui Z. Sheng, MD, PhD, Center for Developmental Biology, 1665 Kong-Jiang Road, Xinhua Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200092, P. R. China. Tel: +86 21 55570361. Fax: +86 21 55570017. e-mail: hzsheng2003@yahoo.com
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Summary

TATA binding protein (TBP) associated factor 1 (TAF1) is a member of the general transcription machinery. Interference in the function of TAF1 causes a broad transcriptional defect in early development. To explore possible roles of TAF1 in embryonic transcriptional silence and zygotic genome activation, we examined the expression of TAF1 in 1-cell mouse embryos. Using an immunofluorescence assay, TAF1 was not detected in embryos in the first few hours after fertilization. TAF1 appeared in pronuclei 6 h post-fertilization and reached a relatively high level before zygotic genome activation. These data show that besides TBP, another critical member of the general transcription machinery such as TAF1 is also absent or at an extremely low level at the outset of development. Combined deficiency in critical members of the general transcription machinery may account for embryonic transcriptional silence.

Keywords

TBPTAF1ZGA
Type
Research Article
Information
Zygote , Volume 14 , Issue 3 , August 2006 , pp. 209 - 215
Copyright
Copyright © Cambridge University Press 2006

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References

Adenot, P.G., Mercier, Y., Renard, J.P. & Thompson, E.M. (1997). Differential H4 acetylation of paternal and maternal chromatin precedes DNA replication and differential transcriptional activity in pronuclei of 1-cell mouse embryos. Development 124, 4615–25.CrossRefGoogle ScholarPubMed
Aoki, F., Worrad, D.M. & Schultz, R.M. (1997). Regulation of transcriptional activity during the first and second cell cycles in the preimplantation mouse embryo. Biol. Reprod. 181, 296–307.Google ScholarPubMed
Apone, L.M. & Green, M.R. (1997). Transcription sans TBP. Nature 393, 114–15.CrossRefGoogle Scholar
Bell, B. & Tora, L. (1999). Regulation of gene expression by multiple forms of TFIID and other novel TAFII-containing complexes. Exp. Cell Res. 246, 11–19.CrossRefGoogle ScholarPubMed
Bouniol, C., Nguyen, E. & Debey, P. (1995). Endogenous transcription occurs at the 1-cell stage in the mouse embryo. Exp. Cell Res. 218, 57–62.CrossRefGoogle ScholarPubMed
Burley, S.K. & Roeder, R.G. (1996). Biochemistry and structural biology of transcription factor IID (TFIID). Annu. Rev. Biochem. 65, 769–99.CrossRefGoogle ScholarPubMed
Hampsey, M. (1998). Molecular genetics of the RNA polymerase II general transcriptional machinery. Microbiol. Mol. Biol. Rev. 62, 465–503.CrossRefGoogle ScholarPubMed
Jordan, P., Mannervik, M., Tora, L. & Carmo-Fonseca, M. (1996). In vivo evidence that TATA-binding protein SL1 colocalizes with UBF and RNA polymerase I when rRNA synthesis is either active or inactive. Cell Biol. 133, 225–34.CrossRefGoogle ScholarPubMed
Kim, J. & Iyer, V.R. (2004). Global role of TAT box-binding protein recruitment to promoters in mediating gene expression profiles. Mol. Cell Biol. 24, 8104–12.CrossRefGoogle Scholar
Kim, J.M., Ogura, A., Nagata, M. & Aoki, F. (2002). Analysis of the mechanism for chromatin remodeling in embryos reconstructed by somatic nuclear transfer. Biol. Reprod. 67, 760–6.CrossRefGoogle ScholarPubMed
Newport, J. & Kirschner, M. (1982 a). A major developmental transition in early Xenopus embryos. I. characterization and timing of cellular changes at the midblastula stage. Cell 30, 675–86.CrossRefGoogle Scholar
Newport, J. & Kirschner, M. (1982 b). A major developmental transition in early Xenopus embryos. II. Control of the onset of transcription. Cell 30, 687–96.CrossRefGoogle Scholar
Ram, P.T. & Schultz, R.M. (1993). Reporter gene expression in G2 of the 1-cell mouse embryo. Dev. Biol. 156, 552–6.CrossRefGoogle ScholarPubMed
Santos, F., Hendrich, B., Reik, W. & Dean, W. (2002). Dynamic reprogramming of DNA methylation in the early mouse embryo. Dev. Biol. 241, 172–82.CrossRefGoogle ScholarPubMed
Schultz, R.M. (2002). The molecular foundations of the maternal to zygotic transition in the preimplantation embryo. Hum. Reprod. Update 8, 323–31.CrossRefGoogle ScholarPubMed
The Jackson Laboratory. http://www.jax.org/cryo/ivf.htmlGoogle Scholar
Tora, L. (2002). A unified nomenclature for TATA box binding protein (TBP)-associated factors (TAFs) involved in RNA polymerase II transcription. Genes Dev. 16, 673–5.CrossRefGoogle ScholarPubMed
Veenstra, G.J., Destree, O.H. & Wolffe, A.P. (1999). Translation of maternal TATA-binding protein mRNA potentiates basal but not activated transcription in Xenopus embryos at the midblastula transition. Mol. Cell Biol. 19, 7972–82.CrossRefGoogle Scholar
Walker, A.K., Shi, Y. & Blackwell, T.K. (2004). An extensive requirement for transcription factor IID-specific TAF-1 in Caenorhabditis elegans embryonic transcription. J. Biol. Chem. 279, 15339–47.CrossRefGoogle ScholarPubMed
Wassarman, D.A. & Sauer, F. (2001). TAF(II) 250: a transcription toolbox. J. Cell Sci. 114, 2895–902.CrossRefGoogle ScholarPubMed
Worrad, D.M., Ram, P.T. & Schultz, R.M. (1994). Regulation of gene expression in the mouse oocyte and early preimplantation embryo: developmental changes in Sp1 and TATA box-binding protein, TBP. Development 120, 2347–57.CrossRefGoogle ScholarPubMed