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Reproductive strategies and chromosomal aberrations affect survival in the Rivuliid fish Hypsolebias sertanejo

Published online by Cambridge University Press:  05 October 2020

Mariana Machado Evangelista ,
Elizabeth Romagosa ,
Diógenes Henrique Siqueira-Silva Open the ORCID record for Diógenes Henrique Siqueira-Silva [Opens in a new window] ,
George Shigueki Yasui ,
Takafumi Fujimoto Open the ORCID record for Takafumi Fujimoto [Opens in a new window]  and
José Augusto Senhorini Open the ORCID record for José Augusto Senhorini [Opens in a new window]
Mariana Machado Evangelista
Affiliation:
Aquaculture Center, São Paulo State University, Jaboticabal, São Paulo, Brazil
Elizabeth Romagosa
Affiliation:
Fishery Institute, SAA, SP, Água Branca, São Paulo, SP, Brasil
Diógenes Henrique Siqueira-Silva*
Affiliation:
Group of Studies on the Reproduction of Amazon fish (GERPA/LANEC), Biology Faculty (FACBIO), University of South and Southern of Pará (Unifesspa), Marabá, Pará, Brazil
George Shigueki Yasui
Affiliation:
National Center for Research and Conservation of Continental Fish, Chico Mendes Institute of Biodiversity Conservation, Pirassununga, São Paulo, Brazil
Takafumi Fujimoto
Affiliation:
Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Japan
José Augusto Senhorini
Affiliation:
National Center for Research and Conservation of Continental Fish, Chico Mendes Institute of Biodiversity Conservation, Pirassununga, São Paulo, Brazil
*
Author for correspondence: Diógenes Henrique Siqueira-Silva. Group of Studies on the Reproduction of Amazon fish (GERPA/LANEC), Biology Faculty (FACBIO), University of South and Southern of Pará (Unifesspa), Marabá, Pará, Brazil. E-mail: siqueira.diogenes@gmail.com
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Summary

Rivulidae comprises a family of fish largely distributed in Brazil that includes 201 species, of which 125 are considered endangered. This fact emphasizes the need for development of conservation strategies including studies on genetics and reproduction. In this paper, we describe aspects of biology and reproduction of the rivuliid species Hypsolebias sertanejo. We outline the reproductive behaviour of this species under laboratory conditions, analyze ploidy status by flow cytometry, describe reproductive behaviour and performance and test dry and wet incubation of eggs. Although H. sertanejo showed well known patterns of reproductive behaviour, we verified many peculiarities inherent to its reproductive biology. As expected, most individuals were diploid (87.71%), however 14.29% were considered mosaics. Although no sterility was observed within mosaics, infertility of these fish was not fully evaluated. Hatching rate of the eggs collected was very low following both dry and wet incubation (5.04 and 3.79%, respectively). These results provide interesting information regarding the reproductive success of this species, and suggest that chromosomal abnormalities described may reduce the survival of H. sertanejo under natural conditions, limiting the perpetuation of this species, and emphasizing the need for more preservation efforts, including artificial propagation and gene banking.

Keywords

BiotechnologyFish biologyFish preservationMosaicismReproduction
Type
Research Article
Information
Zygote , Volume 29 , Issue 1 , February 2021 , pp. 20 - 26
Copyright
© The Author(s), 2020. Published by Cambridge University Press

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