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Response of avian nuclei to mammalian maturation promoting factor (MPF)

Published online by Cambridge University Press:  26 September 2008

P. Trefil ,
M. Horská ,
J. Kaňka ,
J. Fulka ,
J. Jirmanová ,
R.M. Moor  and
J Fulka Jr
P. Trefil
Affiliation:
Institute of Animal Production, Prague, Czech Republic; Czech Academy of Sciences, Liběchov, Czech Republic; The Babraham Institute, Cambridge, UK.
M. Horská
Affiliation:
Institute of Animal Production, Prague, Czech Republic; Czech Academy of Sciences, Liběchov, Czech Republic; The Babraham Institute, Cambridge, UK.
J. Kaňka
Affiliation:
Institute of Animal Production, Prague, Czech Republic; Czech Academy of Sciences, Liběchov, Czech Republic; The Babraham Institute, Cambridge, UK.
J. Fulka
Affiliation:
Institute of Animal Production, Prague, Czech Republic; Czech Academy of Sciences, Liběchov, Czech Republic; The Babraham Institute, Cambridge, UK.
J. Jirmanová
Affiliation:
Institute of Animal Production, Prague, Czech Republic; Czech Academy of Sciences, Liběchov, Czech Republic; The Babraham Institute, Cambridge, UK.
R.M. Moor
Affiliation:
Institute of Animal Production, Prague, Czech Republic; Czech Academy of Sciences, Liběchov, Czech Republic; The Babraham Institute, Cambridge, UK.
J Fulka Jr*
Affiliation:
Institute of Animal Production, Prague, Czech Republic; Czech Academy of Sciences, Liběchov, Czech Republic; The Babraham Institute, Cambridge, UK.
*
J. Fulka, Jr, Institute of Animal Production, Cs-104 00 Prague 10-Uhříněves, Czech Republic. Telephone: 42 2 67710659. Fax 42 2 67710779.
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Summary

Chicken blastodermal cells (stage X) were fused to mouse enucleated oocytes with either no or high maturation promoting factor (MPF) activity. High MPF levels always induced premature chromosome condensation (PCC) irrespective of the number of nuclei fused to a single oocyte. When a single blastodermal cell was fused to a single oocyte without MPF activity the nucleus remained intact for up to 3 h and thereafter underwent PCC. A quite different situation was observed after multiple fusion of several blastodermal cells to a single oocyte without MPF activity. Here, the transplanted nuclei remained intact even after prolonged culture but underwent extensive swelling. DNA synthesis was detected in almost all unfused blastodermal cells. However, after the fusion of several blastodermal cells to a single oocyte no DNA synthesis could be detected. These results provide further evidence that MPF is the universal cell-cycle regulator in the animal kingdom. Its activity is blocked (or neutralised) after fusion to several S-phase cells. Interestingly, our results further suggest that DNA synthesis is suppressed in meiotic cytoplasm even in the presence of an intact nuclear envelope.

Keywords

Avian nucleiChromosome condensationDNA synthesisMeiosis
Type
Article
Information
Zygote , Volume 3 , Issue 4 , November 1995 , pp. 289 - 294
Copyright
Copyright © Cambridge University Press 1995

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