Hostname: page-component-78c5997874-xbtfd Total loading time: 0 Render date: 2024-11-10T17:12:02.416Z Has data issue: false hasContentIssue false
  • English
  • Français

Role of diagnostic intracytoplasmic sperm injection (ICSI) in the management of genetically determined zona pellucida-free oocytes during in vitro fertilization: a case report

Published online by Cambridge University Press:  27 August 2020

Ahmad Metwalley Open the ORCID record for Ahmad Metwalley [Opens in a new window] ,
Nabeel Brasha ,
Sandro C. Esteves ,
Mohamed Fawzy ,
Hadiya Brasha ,
Ali Hellani ,
Manal El Hamshary  and
Omaima Khamiss
Ahmad Metwalley*
Affiliation:
Genetic Engineering and Biotechnology Institute, Sadat City, Egypt IVF Lab Director at Reproductive Medicine and Genetics Unit, Eed Clinic, Jeddah, KSA
Nabeel Brasha
Affiliation:
Consultant Reproductive Medicine and in vitro fertilization, National Guard Hospital, Jeddah, KSA
Sandro C. Esteves
Affiliation:
ANDROFERT, Andrology and Human Reproduction Clinic, Referral Center for Male Reproduction, Campinas, SP, Brazil
Mohamed Fawzy
Affiliation:
IbnSina and Banon IVF Centers, IbnSina Hospital, El Aref Squeer, Sohag, Egypt
Hadiya Brasha
Affiliation:
Consultant Reproductive Medicine and in vitro fertilization, National Guard Hospital, Jeddah, KSA
Ali Hellani
Affiliation:
Viafet Genomic Laboratories
Manal El Hamshary
Affiliation:
Genetic Engineering and Biotechnology Institute, Sadat City, Egypt
Omaima Khamiss
Affiliation:
Genetic Engineering and Biotechnology Institute, Sadat City, Egypt
*
Author for correspondence: Ahmad Metwalley. IVF Lab Director, Reproductive Medicine and Genetics Unit, Eed Clinic, Jeddah, KSA. Tel: +966 567567556. E-mail: ahmadmetwalley@yahoo.com
Get access Rights & Permissions [Opens in a new window]

Abstract

Purpose:

To report the utilization of diagnostic intracytoplasmic sperm injection (D-ICSI), an ICSI cycle performed in the natural cycle, to obtain information about embryo development potential after sperm injection into zona pellucida (ZP)-free oocytes.

Materials and Methods:

We report the case of a couple with primary unexplained infertility with a history of previous failed, in vitro fertilization intracytoplasmic sperm injection (IVF-ICSI) cycles characterized by the presence of ZP-free oocytes. Whole exome sequencing (WES) was carried out to analyse the possible genetic basis of oocyte abnormality.

Results:

Diagnostic ICSI provided information about the embryo development potential from ZP-free oocytes and allowed better planning of the subsequent ICSI cycle. WES revealed that the absence of ZP was likely to be due to a new (ZP1) mutation. The subsequent ICSI cycle resulted in the delivery of a healthy baby.

Discussion:

To the best of our knowledge, our report is the first to describe the use of D-ICSI to determine the feasibility of embryo development and implantation in a patient with ZP1 mutation, resulting in the subsequent delivery of a healthy baby. We used ‘diagnostic’ ICSI in the normal menstrual cycle to explore the feasibility of embryo development after sperm injection into ZP-free oocytes. Our results may expand the spectrum of diagnostic procedures associated with unexplained infertility.

Keywords

Diagnostic intracytoplasmic sperm injectionZona-free oocyteZona pellucidaZona pellucida gene mutationZP1ZP abnormality
Type
Method
Information
Zygote , Volume 28 , Issue 6 , December 2020 , pp. 519 - 523
Copyright
© The Author(s), 2020. Published by Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Asa, E, Tabatabaee, R, Farrokhi, A and Nejatbakhsh, R (2017). Relationship between meiotic spindles visualization and intracytoplasmic sperm injection outcomes in human oocytes. Anatom Cell Biol 50, 26–32.CrossRefGoogle ScholarPubMed
Balaban, B and Urman, B (2006). Effect of oocyte morphology on embryo development and implantation. Reprod Biomed Online 12, 608–15.CrossRefGoogle ScholarPubMed
Balaban, B Brison, D, Calderon, G, Catt, J, Conaghan, J, Cowan, L, Ebner, T, Gardner, D, Hardarson, T and Lundin, K (2011). Alpha scientists in reproductive medicine and ESHRE special interest group of embryology. The Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting. Hum Reprod 26 1270–83.Google Scholar
Dai, C, Chen, Y, Hu, L, Du, J, Gong, F, Dai, J, Zhang, S, Wang, M, Chen, J and Guo, J (2019a). ZP1 mutations are associated with empty follicle syndrome: evidence for the existence of an intact oocyte and a zona pellucida in follicles up to the early antral stage. A case report. Hum Reprod 34, 2201–7.Google Scholar
Dai, C, Hu, L, Gong, F, Tan, Y, Cai, S, Zhang, S, Dai, J, Lu, C, Chen, J and Chen, Y (2019b). ZP2 pathogenic variants cause in vitro fertilization failure and female infertility. Genet Med 21, 431–40.CrossRefGoogle ScholarPubMed
de Vos, A, van Landuyt, L, van Ranst, H, Vandermonde, A, D’Haese, V, Sterckx, J, Haentjens, P, Devroey, P and van der Elst, J (2008). Randomized sibling-oocyte study using recombinant human hyaluronidase versus bovine-derived Sigma hyaluronidase in ICSI patients. Hum Reprod 23, 1815–9.CrossRefGoogle ScholarPubMed
Ebner, T, Moser, M and Tews, G (2006). Is oocyte morphology prognostic of embryo developmental potential after ICSI? Reprod Biomed Online 12, 507–12.CrossRefGoogle ScholarPubMed
Ebner, T Shebl, O, Moser, M, Sommergruber, M and Tews, G (2008). Developmental fate of ovoid oocytes. Hum Reprod 23, 62–6.CrossRefGoogle ScholarPubMed
El-Mestrah, M, Castle, P E, Borossa, G and Kan, FWK (2002). Subcellular distribution of ZP1 ZP2 and ZP3 glycoproteins during folliculogenesis and demonstration of their topographical disposition within the zona matrix of mouse ovarian oocytes. Biol Reprod 66, 866–76.CrossRefGoogle ScholarPubMed
Esfandiari, N, Ryan, EA J, Gotlieb, L and Casper, RF (2005). Successful pregnancy following transfer of embryos from oocytes with abnormal zona pellucida and cytoplasm morphology. Reprod Biomed Online 11, 620–3.CrossRefGoogle ScholarPubMed
Evison, M, Pretty, C, Taylor, E and Franklin, C (2009). Human recombinant hyaluronidase (Cumulase®) improves intracytoplasmic sperm injection survival and fertilization rates. Reprod Biomed Online 18, 811–4.CrossRefGoogle Scholar
Ezra, Y, Simon, A and Laufer, N (1992). Defective oocytes: a new subgroup of unexplained infertility. Fertil Steril 58, 24–7.CrossRefGoogle ScholarPubMed
Hu, Y and Trolice, MP (2016). Live birth of twins derived from zona-free oocytes. Fertil Steril 105, 1232–5.CrossRefGoogle ScholarPubMed
Mahadevan, MM and Trounson, AO (1985). Removal of the cumulus oophorus from the human oocyte for in vitro fertilization. Fertil Steril 43, 263–7.CrossRefGoogle ScholarPubMed
Mortimer, D (1991). Sperm Preparation Techniques and Iatrogenic Failures of In Vitro Fertilization. Citeseer.CrossRefGoogle ScholarPubMed
World Health Organization (2010). WHO Laboratory Manual for the Examination and Processing of Human Semen.Google Scholar
Paz, G, Amit, A and Yavetz, H (2004). Case report: pregnancy outcome following ICSI of oocytes with abnormal cytoplasm and zona pellucida. Hum Reprod 19, 586–9.CrossRefGoogle ScholarPubMed
Rankin, T and Dean, J (1996). Preimplantation embryology: The molecular genetics of the zona pellucida: mouse mutations and infertility. MHR: Basic Sci Reprod Med 2, 889–94.Google Scholar
Richards, S, Aziz, N, Bale, S, Bick, D, Das, S, Gastier-Foster, J, Grody, WW, Hegde, M, Lyon, E and Spector, E (2015). Standards and guidelines for the interpretation of sequence variants: a joint consensus recommendation of the American College of Medical Genetics and Genomics and the Association for Molecular Pathology. Genet Med 17, 405–23.CrossRefGoogle ScholarPubMed
Stanger, J D, Stevenson, K, Lakmaker, A and Woolcott, R (2001). Pregnancy following fertilization of zona-free coronal cell intact human ova: case report. Hum Reprod 16, 164–7.CrossRefGoogle ScholarPubMed
Vajta, G, Rienzi, L and Bavister, BD (2010). Zona-free embryo culture: is it a viable option to improve pregnancy rates? Reprod Biomed Online 21, 17–25.CrossRefGoogle ScholarPubMed
Vassena, R, Blazquez, A, Guillén, J, Colomé, C, Mataró, D and Vernaeve, V (2014). Empty Follicle Syndrome (EFS) prevalence and management. Reprod Biomed Online 28 S18–9.CrossRefGoogle Scholar
Yang, P, Luan, X, Peng, Y, Chen, T, Su, S, Zhang, C, Wang, Z, Cheng, L, Zhang, X and Wang, Y (2017). Novel zona pellucida gene variants identified in patients with oocyte anomalies. Fertil Steril 107, 1364–9.CrossRefGoogle ScholarPubMed
Yu, E J, Ahn, H, Lee, J M, Jee, B C and Kim, SH (2015). Fertilization and embryo quality of mature oocytes with specific morphological abnormalities. Clin Exp Reprod Med 42, 156.CrossRefGoogle ScholarPubMed
Zhang, Z, Shangguan, T, Li, Y-Y and He, W (2018). Infertility due to lack of zona pellucida caused by a compound heterozygous mutation in ZP1 gene. Reprod Dev Med 2, 183.Google Scholar