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Round spermatids stained with MitoTracker can be used to produce offspring more simply

Published online by Cambridge University Press:  19 May 2005

Takafusa Hikichi ,
Satoshi Kishigami ,
Nguyen Van Thuan ,
Hiroshi Ohta ,
Eiji Mizutani ,
Sayaka Wakayama  and
Teruhiko Wakayama
Takafusa Hikichi
Affiliation:
Center for Developmental Biology, RIKEN Kobe, 2-2-3 Minatojima-minamimachi, Kobe 650-0047, Japan.
Satoshi Kishigami
Affiliation:
Center for Developmental Biology, RIKEN Kobe, 2-2-3 Minatojima-minamimachi, Kobe 650-0047, Japan.
Nguyen Van Thuan
Affiliation:
Center for Developmental Biology, RIKEN Kobe, 2-2-3 Minatojima-minamimachi, Kobe 650-0047, Japan.
Hiroshi Ohta
Affiliation:
Center for Developmental Biology, RIKEN Kobe, 2-2-3 Minatojima-minamimachi, Kobe 650-0047, Japan.
Eiji Mizutani
Affiliation:
Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan.
Sayaka Wakayama
Affiliation:
Department of Life Science, Graduate School of Science and Technology, Kobe University, Kobe 657-8501, Japan.
Teruhiko Wakayama
Affiliation:
Center for Developmental Biology, RIKEN Kobe, 2-2-3 Minatojima-minamimachi, Kobe 650-0047, Japan.
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Abstract

Although both intracytoplasmic sperm injection (ICSI) and round spermatid injection (ROSI) are used in infertility treatments, the rate of offspring achieved with ROSI is low compared with that achieved with ICSI. The difficulty in correctly selecting round spermatids from testicular cells is one of the causes of this phenomenon. We easily selected live round spermatids from testicular cells stained with 20 nM MitoTracker, which visualizes mitochondria without killing the cell. Using this method, we divided round spermatids into three groups based on the polarization of their mitochondria, and performed ROSI. The rate of successful offspring achieved with MitoTracker-stained ROSI was the same in all groups. This indicates that changes in the polarization of mitochondria in round spermatids are not directly related to the developmental capacity of subsequently fertilized embryos. Because this staining has no harmful effects on embryo development, the selection of spermatids by MitoTracker under a fluorescence microscope should be useful in research into and the treatment of infertility.

Keywords

FertilizationMitoTrackerOocyteROSIRound spermatid
Type
Research Article
Information
Zygote , Volume 13 , Issue 1 , February 2005 , pp. 55 - 61
Copyright
2005 Cambridge University Press

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