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Seminal characterization of the Amazonian fire-eye tetra Moenkhausia oligolepis (Günther, 1864)

Published online by Cambridge University Press:  19 August 2020

Jeane Rodrigues ,
Raquel Santos dos Santos ,
Jhennifer Gomes Cordeiro ,
Marissol Leite ,
Hingrid Suzzan Tarso Oliveira e Oliveira ,
Hadda Tercya ,
Bruna Patrícia Dutra Costa ,
Nivaldo Ferreira do Nascimento Open the ORCID record for Nivaldo Ferreira do Nascimento [Opens in a new window] ,
Caio Maximino  and
Diógenes Henrique de Siqueira-Silva Open the ORCID record for Diógenes Henrique de Siqueira-Silva [Opens in a new window]
Jeane Rodrigues
Affiliation:
Group of Studies on the Reproduction of Amazon fish (GERPA/LaNeC), Biology Faculty (FACBIO), University Federal of South and Southern of Pará (Unifesspa), Marabá, Pará, Brazil Post-Graduation Program in Amazonian Animal Reproduction – ReproAmazon – UFPA/Ufra Belem, Pará, Brazil
Raquel Santos dos Santos
Affiliation:
Group of Studies on the Reproduction of Amazon fish (GERPA/LaNeC), Biology Faculty (FACBIO), University Federal of South and Southern of Pará (Unifesspa), Marabá, Pará, Brazil
Jhennifer Gomes Cordeiro
Affiliation:
Group of Studies on the Reproduction of Amazon fish (GERPA/LaNeC), Biology Faculty (FACBIO), University Federal of South and Southern of Pará (Unifesspa), Marabá, Pará, Brazil PPG in Biodiversity and Biotechnology (BIONORTE)
Marissol Leite
Affiliation:
Group of Studies on the Reproduction of Amazon fish (GERPA/LaNeC), Biology Faculty (FACBIO), University Federal of South and Southern of Pará (Unifesspa), Marabá, Pará, Brazil
Hingrid Suzzan Tarso Oliveira e Oliveira
Affiliation:
Group of Studies on the Reproduction of Amazon fish (GERPA/LaNeC), Biology Faculty (FACBIO), University Federal of South and Southern of Pará (Unifesspa), Marabá, Pará, Brazil
Hadda Tercya
Affiliation:
Group of Studies on the Reproduction of Amazon fish (GERPA/LaNeC), Biology Faculty (FACBIO), University Federal of South and Southern of Pará (Unifesspa), Marabá, Pará, Brazil
Bruna Patrícia Dutra Costa
Affiliation:
Group of Studies on the Reproduction of Amazon fish (GERPA/LaNeC), Biology Faculty (FACBIO), University Federal of South and Southern of Pará (Unifesspa), Marabá, Pará, Brazil PPG in Biodiversity and Biotechnology (BIONORTE)
Nivaldo Ferreira do Nascimento
Affiliation:
Laboratory of Fish Biotechnology, National Center for Research and Conservation of Continental Fish, Chico Mendes Institute of Biodiversity Conservation, Rodovia Pref. Euberto Nemésio Pereira de Godoy, Pirassununga, SP13630-970, Brazil
Caio Maximino
Affiliation:
Group of Studies on the Reproduction of Amazon fish (GERPA/LaNeC), Biology Faculty (FACBIO), University Federal of South and Southern of Pará (Unifesspa), Marabá, Pará, Brazil PPG in Biodiversity and Biotechnology (BIONORTE)
Diógenes Henrique de Siqueira-Silva*
Affiliation:
Group of Studies on the Reproduction of Amazon fish (GERPA/LaNeC), Biology Faculty (FACBIO), University Federal of South and Southern of Pará (Unifesspa), Marabá, Pará, Brazil PPG in Biodiversity and Biotechnology (BIONORTE) Post-Graduation Program in Amazonian Animal Reproduction – ReproAmazon – UFPA/Ufra Belem, Pará, Brazil
*
Author for correspondence: Diógenes Henrique de Siqueira-Silva. Group of Studies on the Reproduction of Amazon fish (GERPA/LaNeC), Biology Faculty (FACBIO), University Federal of South and Southern of Pará (Unifesspa), Marabá, Pará, Brazil. E-mail: siqueira.diogenes@gmail.com, diogenessilva@unifesspa.edu.br
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Summary

The seminal characteristics of Moenkhausia oligolepis are described. Three males were induced with a single dose of carp pituitary. Semen was collected 6 h after induction, and diluted in dibasic sodium phosphate extender solution. For motility analysis, 1 µl of diluted semen was added to 10 µl of distilled water to achieve gamete activation. The average duration of total motility was 76.67 s; while the average sperm motility rate at intervals of 15 s was 95.3, 85.3, 59.6, 31.7, 13.0, 4.6 and 1.2%. To determine sperm concentration in samples, 0.5 μl of semen was diluted with 500 μl of glutaraldehyde. An aliquot of 10 μl of this dilution was utilized for cell counting. An average count of 4.97 × 109 ± 3.46 sperm/ml was obtained. Morphological analyses were performed using eosin–nigrosine dye; 20.33% of the sperm were observed to be dead. Live sperm, comprising the other 79.67%, had an average length of approximately 30 µm, with a head diameter of 4.488 ± 0.7 µm; and a flagella plus mid-piece length of 26.071 ± 12.4 µm. Of those sperm, 69% had a normal morphology, while 31% had primary and secondary abnormalities. The observed abnormality rate did not have a detrimental effect on artificial fertilization potential for the species. The description of the seminal characteristics of a species is one of the most important sets of information required for artificial reproduction of fish in captivity. It also contributes significantly to the total biological knowledge of the studied species.

Keywords

Amazonian fishArtificial fertilizationFish biotechnologySemen analysisSpermatozoa
Type
Research Article
Information
Zygote , Volume 28 , Issue 6 , December 2020 , pp. 453 - 458
Copyright
© The Author(s), 2020. Published by Cambridge University Press

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