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Short-term storage of Chinese sturgeon (Acipenser sinensis) ova in vitro

Published online by Cambridge University Press:  15 January 2021

Chun Tan Open the ORCID record for Chun Tan [Opens in a new window] ,
Xueqing Liu ,
Jing Yang ,
Juanjuan Liu  and
Hejun Du
Chun Tan
Affiliation:
Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei443100, China Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei443100, China
Xueqing Liu
Affiliation:
Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei443100, China Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei443100, China
Jing Yang
Affiliation:
Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei443100, China Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei443100, China
Juanjuan Liu
Affiliation:
Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei443100, China Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei443100, China
Hejun Du*
Affiliation:
Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei443100, China Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei443100, China
*
Author for correspondence: Hejun Du. Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei443100, China. Tel: +86 7176713481. Fax: +86 7176713481. E-mail: du_hejun@ctg.com.cn
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Summary

In this study, we tried to maintain the vitality of Chinese sturgeon (Acipenser sinensis) ova before fertilization with several treatments in vitro. The ovulated eggs were allocated to groups with different incubation medium (coelomic fluid and artificial media), temperature (4°C and 16°C) and storage duration (2 h and 6 h). The maximum fertilization and hatching rate were observed for the control group in which the ova were fertilized immediately after spawning, with the values of 82.45% and 84.73%, respectively. Compared with the control group, the fertilization and hatching rate of all the treatment groups stored at 4°C or in coelomic fluid decreased significantly (P < 0.05). The fertilization rate of the treatment group stored in artificial medium at 16°C did not change obviously in the first 2 h (P > 0.05), but declined dramatically (P < 0.05) after 6 h. In comparison with the control group, no significant (P > 0.05) reduction was shown in hatching rate of the treatment group stored in artificial medium at 16°C for 6 h. The results showed that the ova of Chinese sturgeon can be stored for at least 6 h at 16°C in artificial medium without weakening; this provides a practical application method for the routine hatchery practice of Chinese sturgeon, as well as certain relevant research.

Keywords

Artificial mediumChinese sturgeonCoelomic fluidOvaStorage
Type
Short Communication
Information
Zygote , Volume 29 , Issue 3 , June 2021 , pp. 245 - 248
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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References

Billard, R, Cosson, J, Noveiri, SB and Pourkazemi, M (2004). Cryopreservation and short-term storage of sturgeon sperm, a review. Aquaculture 236, 1–9.CrossRefGoogle Scholar
Bobe, J (2015). Egg quality in fish: present and future challenges. Anim Front 5, 66–72.CrossRefGoogle Scholar
Bozkurt, Y and Yavas, I (2012). Effect of temperatures and storage periods on fertilizing and hatching of short-term preserved scaly carp (Cyprinus carpio) eggs. Isr J Aquac-Bamidgeh 64, 1–5.Google Scholar
Ciereszko, A, Glogowski, J and Dabrowski, K (2000). Fertilization in landlocked sea lamprey: storage of gametes, optimal sperm: egg ratio, and methods of assessing fertilization success. J Fish Biol 56, 495–505.Google Scholar
Formacion, MJ, Venkatesh, B, Tan, CH and Lam, TJ (1995). Overripening of ovulated eggs in goldfish, Carassius auratus: II. Possible involvement of postovulatory follicles and steroids. Fish Physiol Biochem 14, 237–46.CrossRefGoogle ScholarPubMed
Gisbert, E and Williot, P (2002). Influence of storage duration of ovulated eggs prior to fertilisation on the early ontogenesis of sterlet (Acipenser ruthenus) and Siberian sturgeon (Acipenser baeri). Int Rev Hydrobiol 87, 605–12.3.0.CO;2-#>CrossRefGoogle Scholar
Goetz, FW and Coffman, MA (2000). Storage of unfertilized eggs of rainbow trout (Oncorhynchus mykiss) in artificial media. Aquaculture 184, 267–76.CrossRefGoogle Scholar
Guan, M, Rawson, DM and Zhang, T (2008). Cryopreservation of zebrafish (Danio rerio) oocytes using improved controlled slow cooling protocols. Cryobiology 56, 204–8.CrossRefGoogle ScholarPubMed
Guo, BF, Chang, JB, Xiao, H, Zhu, B, Wan, JY, Tian, JY and Shu, DB (2011). Study on the characteristics of thorough artificial reproduction of Chinese sturgeon for the first time. Acta Hydrobiol Sin 35, 940–5. (in Chinese)Google Scholar
Hagedorn, M, Kleinhans, FW, Artemov, D and Pilatus, U (1998). Characterization of a major permeability barrier in the zebrafish embryo 1. Biol Reprod 59, 1240–50.CrossRefGoogle Scholar
Kagawa, H, Young, G and Nagahama, Y (1984). In vitro estradiol-17β and testosterone production by ovarian follicles of the goldfish, Carassius auratus . Gen Comp Endocr 54, 139–43.CrossRefGoogle ScholarPubMed
Linhart, O, Shelton, WL, Tuckova, V, Rodina, M and Siddique, M (2016). Effects of temperature on in vitro short-term storage of sterlet sturgeon (Acipenser ruthenus) ova. Reprod Domest Anim 51, 165–70.CrossRefGoogle ScholarPubMed
Lubzens, E, Young, G, Bobe, J and Cerda, J (2010). Oogenesis in teleosts: how fish eggs are formed. Gen Comp Endocr 165, 367–89.CrossRefGoogle ScholarPubMed
Nazari, R M, Modanloo, M, Ghomi, MR and Ovissipor, MR (2010). Application of synthetic hormone LHRH-A2 on the artificial propagation of Persian sturgeon Acipenser persicus . Aquacult Int 18, 837–41.CrossRefGoogle Scholar
Nguenga, D, Teugels, GG, Legendre, M and Ollevier, F (2004). Effects of storage and incubation temperature on the viability of eggs, embryos and larvae in two strains of an African catfish, Heterobranchus longifilis (Siluriformes, Clariidae). Aquac Res 35, 1358–69.CrossRefGoogle Scholar
Niksirat, H, Sarvi, K, Amiri, BM, Karami, M and Hatef, A (2007). In vitro storage of unfertilized ova of endangered Caspian brown trout (Salmo trutta caspius) in artificial media. Anim Reprod Sci 100, 356–63.CrossRefGoogle Scholar
Raymakers, C (2002). International trade in sturgeon and paddlefish species – the effect of CITES listing. Internat Rev Hydrobiol 87, 525–37.3.0.CO;2-W>CrossRefGoogle Scholar
Richardson, GF, Gardiner, YT and McNiven, MA (2002). Preservation of rainbow trout (Oncorhynchus mykiss) eyed eggs using a perfluorochemical as an oxygen carrier. Theriogenology 58, 1283–90.CrossRefGoogle Scholar
Rizzo, E, Godinho, HP and Sato, Y (2003). Short-Term storage of oocytes from the neotropical teleost fish Prochilodus marggravii . Theriogenology 60, 1059–70.CrossRefGoogle ScholarPubMed
Robles, V, Cabrita, E and Herraez, MP (2009). Germplasm cryobanking in zebrafish and other aquarium model species. Zebrafish 6, 281–93.CrossRefGoogle ScholarPubMed
Samarin, AM, Policar, T and Lahnsteiner, F (2015). Fish oocyte ageing and its effect on egg quality. Rev Fish Sci Aquac 23, 302–14.CrossRefGoogle Scholar
Samarin, AM, Zarski, D, Palinska-Zarska, K, Krejszeff, S, Blecha, M, Kucharczyk, D and Policar, T (2017). In vitro storage of unfertilized eggs of the Eurasian perch and its effect on egg viability rates and the occurrence of larval malformations. Animal 11, 78–83.CrossRefGoogle ScholarPubMed
Shen, YX, Wang, PF, Wang, C, Yu, Y and Kong, N (2018). Potential causes of habitat degradation and spawning time delay of the Chinese sturgeon (Acipenser sinensis). Ecol Inform 43, 96–105.CrossRefGoogle Scholar
Sohrabnezhad, M, Kalbassi, M R, Nazari, RM and Bahmani, M (2006). Short-Term storage of Persian sturgeon (Acipenser persicus) ova in artificial media and coelomic fluid. J Appl Ichthyol 22, 395–9.CrossRefGoogle Scholar
Tsai, S and Lin, C (2009). Effects of cryoprotectant on the embryos of banded coral shrimp (Stenopus hispidus): preliminary studies to establish freezing protocols. Cryoletters 30, 373–81.Google ScholarPubMed
Wang, C, Kynard, B, Wei, Q, Du, H and Zhang, H (2013). Spatial distribution and habitat suitability indices for non-spawning and spawning adult Chinese sturgeons below Gezhouba dam, Yangtze River: effects of river alterations. J Appl Ichthyol, 29, 31–40.CrossRefGoogle Scholar
Wei, QW, Ke, FE, Zhang, JM, Zhuang, P, Luo, J D, Zhou, RQ and Yang, WH (1997). Biology, fisheries, and conservation of sturgeons and paddlefish in China. Environ Biol Fish 48, 241–56.CrossRefGoogle Scholar
Yi, Y J, Sun, J and Zhang, SH (2016a). A habitat suitability model for Chinese sturgeon determined using the generalized additive method. J Hydrol 534, 11–18.CrossRefGoogle Scholar
Yi, Y J, Sun, J, Zhang, SH and Yang, ZF (2016b). Assessment of Chinese sturgeon habitat suitability in the Yangtze River (China): comparison of generalized additive model, data-driven fuzzy logic model, and preference curve model. J Hydrol 536, 447–56.CrossRefGoogle Scholar
Zhuang, P, Zhao, F, Zhang, T, Chen, Y, Liu, JY, Zhang, LZ and Kynard, B (2016). New evidence may support the persistence and adaptability of the near-extinct Chinese sturgeon. Biol Conserv 193, 66–9.CrossRefGoogle Scholar