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Somatic cell nuclear transfer using transported in vitro-matured oocytes in cynomolgus monkey

Published online by Cambridge University Press:  01 February 2007

N. Chen ,
S-L. Liow ,
R. Bin Abdullah ,
WK. Khadijah Wan Embong ,
W-Y. Yip ,
L-G. Tan ,
G-Q. Tong  and
S-C. Ng
N. Chen
Affiliation:
Embryonics International, Gleneagles Hospital, Singapore 258500, Singapore.
S-L. Liow
Affiliation:
Embryonics International, Gleneagles Hospital, Singapore 258500, Singapore.
R. Bin Abdullah
Affiliation:
Department of Biological Sciences, University of Malaya, Kuala Lumpur 50603, Malaysia.
WK. Khadijah Wan Embong
Affiliation:
Department of Biological Sciences, University of Malaya, Kuala Lumpur 50603, Malaysia.
W-Y. Yip
Affiliation:
Embryonics International, Gleneagles Hospital, Singapore 258500, Singapore.
L-G. Tan
Affiliation:
Department of Obstetrics & Gynaecology, National University of Singapore, Singapore 119074, Singapore.
G-Q. Tong
Affiliation:
Department of Obstetrics & Gynaecology, National University of Singapore, Singapore 119074, Singapore.
S-C. Ng*
Affiliation:
Embryonics International, Gleneagles Hospital, Singapore 258500, Singapore. Department of Obstetrics & Gynaecology, National University of Singapore, Singapore 119074, Singapore.
*
All correspondence to: S-C. Ng, Gleneagles Hospital, Annex Block, #01–38, 6A Napier Road, Singapore 258500, Singapore. Tel: +65 6479 7267. Fax: +65 6479 6536. e-mail: scng@embryonics.biz
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Summary

Somatic cell nuclear transfer (SCNT) is not successful so far in non-human primates. The objective of this study was to investigate the effects of stimulation cycles (first and repeat) on oocyte retrieval and in vitro maturation (IVM) and to evaluate the effects of stimulation cycles and donor cell type (cumulus and fetal skin fibroblasts) on efficiency of SCNT with transported IVM oocytes. In this study, 369 immature oocytes were collected laparoscopically at 24 h following human chorionic gonadotrophin (hCG) treatment from 12 cynomolgus macaque (Macaca fascicularis) in 24 stimulation cycles, and shipped in pre-equilibrated IVM medium for a 5 h journey, placed in a dry portable incubator (37 °C) without CO2 supplement. A total of 70.6% (247/350) of immature oocytes reached metaphase II (MII) stage at 36 h after hCG administration, MII spindle could be seen clearly in 80.6% (104/129) of matured IVM oocytes under polarized microscopy. A total of 50.0% (37/74) of reconstructive SCNT embryos cleaved after activation; after cleavage, 37.8% (14/37) developed to the 8-cell stage and 8.1% (3/37) developed to morula, but unfortunately none developed to the blastocyst stage. Many more oocytes could be retrieved per cycle from monkeys in the first cycle than in repeated cycles (19.1 vs. 11.7, p < 0.05). There were no significant differences in the maturation rate (70.0 vs. 71.4%, p > 0.05) and MII spindle rate under polarized microscopy (76.4 vs. 86.0%, p > 0.05) between the first and repeat cycles. There were also no significant differences in the cleavage rate, and the 4-cell, 8-cell and morula development rate of SCNT embryos between the first and repeat cycles. When fibroblast cells and cumulus cells were used as the donor cells for SCNT, first cleavage rate was not significantly different, but 4-cell (50.0 vs. 88.9%, p < 0.05) and 8-cell (0 vs. 51.9%, p < 0.01) development rate were significantly lower for the former. In conclusion, the number of stimulation cycles has a significant effect on oocyte retrieval, but has no effect on maturation and SCNT embryo development; however, different donor cell types (cumulus and fibroblast) resulted in different developmental potentials of SCNT embryos.

Keywords

CloningIn vitro maturationMonkeyNuclear transferOocytesTransport
Type
Research Article
Information
Zygote , Volume 15 , Issue 1 , February 2007 , pp. 25 - 33
Copyright
Copyright © Cambridge University Press 2007

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