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Sperm abnormalities in matrinxã Brycon amazonicus after hormonal treatment with carp pituitary extract and Ovaprim™

Published online by Cambridge University Press:  17 September 2018

Rosilane Gomes de Souza de Oliveira ,
Arley Faria José de Oliveira  and
Marle Angélica Villacorta Correa
Rosilane Gomes de Souza de Oliveira*
Affiliation:
Universidade Nilton Lins/Instituto Nacional de Pesquisas da Amazônia, Programa de Pós-graduação em Aquicultura
Arley Faria José de Oliveira
Affiliation:
Universidade Federal do Amazonas, Instituto de Ciências Biológicas, Av. Gen. Rodrigo Octávio Jordão Ramos, 3000. CEP: 69077-000 – Manaus, Amazonas, Brazil
Marle Angélica Villacorta Correa
Affiliation:
Universidade Federal do Amazonas, Faculdade de Ciências Agrárias, Departamento de Ciências Pesqueiras, Av. Gen. Rodrigo Octávio Jordão Ramos, 3000. CEP: 69077-000 – Manaus, Amazonas, Brazil
*
Author for correspondence: Rosilane Gomes de Souza de Oliveira – Universidade Nilton Lins, Av. Nilton Lins, 3259, Manaus-AM, CEP: 69058-030 – Manaus, Amazonas, Brazil. Tel: +55 92–36432022. Fax: +55 92–36432022. E-mail: rosilanegso@gmail.com
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Summary

The aim of this study was to evaluate if hormonal treatment causes changes in the morphology of matrinxã Brycon amazonicus spermatozoa. Twelve males were randomized into three treatment groups: 1, Ovaprim™ (0.5 ml/kg); 2, carp pituitary extract (CPE; 2.0 mg/kg); and 3, NaCl solution 0.9% – control group (0.5 ml/kg); with four replicates. Morphological sperm analysis was performed using an optical and scanning electron microscope. The percentage of normal spermatozoa (49.6±4.6% to 60.8±2.8%), with primary (26.1±6.4% to 45.3±4.5%) and secondary abnormalities (4.4±2.9% to 13.9±3.5%) did not differ significantly between treatment groups. There were no significant differences between treatments in relation to the primary abnormalities found in the head (10.5±3.8% to 25.5±6.3%), the midpiece (0.1±0.1% to 0.2±0.2%) and in the flagellum (18.9±3.8% to 15.5±3.1%), as well as in the secondary abnormalities, located in the head (0.8±0.4% to 7.8±4.6%) and in the flagellum (2.2±1.1% to 6.1±1.5%). When each abnormality was evaluated individually, only the percentage of degenerated head was higher in the CPE-induced group (24.9±5.9%) than the control group (7.2±3.1%). We concluded that the use of pituitary extract of carp or Ovaprim™ under the conditions of this experiment does not influence the percentage of sperm abnormalities in B. amazonicus.

Keywords

FishInducing hormonesMicroscopeMorphological changesSpermatozoa
Type
Research Article
Information
Zygote , Volume 26 , Issue 4 , August 2018 , pp. 279 - 285
Copyright
© Cambridge University Press 2018 

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References

Araújo, JEXS, Streit, DP Jr, Ribeiro, JSA, Martins, EFF, Souza, FN, Oliveira, CAL, Ribeiro, RP, Lopera-Barrero, NM Povh, JA (2014) Ovopel and carp pituitary extract as spawning inducers in males of the Amazon catfish Leiarius marmoratus (Gill, 1970). Braz Arch Biol Technol 57, 882–886.Google Scholar
Asturiano, JF, Marco-Jiménez, F, Pérez, L, Balasch, S, Garzon, DL, Peñaranda, DS, Vicente, JS, Viudes-de-Castro, MP Jover, M (2006) Effects of hCG as spermiation inducer on European eel semen quality. Theriogenology 66, 1012–1020.Google Scholar
Bashiyo-Silva, C, Costa, RS, Ribeiro, DC, Senhorini, JA, Veríssimo-Silveira, R Ninhaus-Silveira, A (2016) Hormonal induction of Brycon cephalus (Characiformes, Characidae) to spermiation using d-ala6, pro9net-mGnRH + metoclopramide. Zygote 24, 319–325.Google Scholar
Bombardelli, RA, Mörschbächer, EF, Campagnolo, R, Sanches, EA Syperreck, MA (2006) Dose inseminante para fertilização artificial de ovócitos de jundiá Rhamdia quelen (Quoy & Gaimardm, 1824). Rev Bras Zootec 35, 125–257.Google Scholar
Bromage, N (1995) Broodstock management and seed quality – general considerations. In: Broodstock Management and Egg Larval Quality (eds NE Bromage and RJ Roberts), pp. 1–24. Oxford: Blackwell Science.Google Scholar
Cejko, BI, Żarski, D, Krejszeff, S, Kucharczyk, D Kowalski, RK (2013) Effect of hormonal stimulation on milt volume, number of sperm and sperm motility in the crucian carp, Carassius carassius (L.). Isr J of Aquacult 65, 1–7.Google Scholar
Colégio Brasileiro De Reprodução Animal – CBRA (2013) Manual para exames andrológicos e avaliação de sêmen animal 3rd edn, Belo Horizonte, MG, 104 pp.Google Scholar
Cosson, J, Dreanno, C, Billard, R, Suquet, M Cibert, C (1999) Regulation of axonemal wave parameters of fish spermatozoa by ionic factors. In: The Male Gamete: From Basic Science to Clinical Applications (ed. C Gagnon), pp. 161–186. Montréal: Cache River Press.Google Scholar
De Alexandre, JS, Ninhaus-Silveira, A, Veríssimo-Silveira, R, Buzollo, H, Senhorini, JA Chaguri, MP (2010) Structural analysis of the embryonic development in Brycon cephalus (Günther, 1869). Zygote 18, 173–183.Google Scholar
Donaldson, EM, Solar, II Harvey, B (2000) Induced ovulation and spermiation and factors influencing gamete quality of fishes. In: Cryopreservation in Aquatic Species (eds TR Tiersch and PM Mazik), pp. 13–19. Baton Rouge, LA, USA: The World Aquaculture Society.Google Scholar
Fauvel, C, Suquet, M Cosson, J (2010) Evaluation of fish sperm quality. J Appl Ichthyol 26, 636–643.Google Scholar
Freneau, GE (2011) Aspectos da morfologia espermática em touros. Rev Bras Reprod An 35, 160–170.Google Scholar
Garcia, RRF, Vasconcelos, ACN, Povh, JA, Ribeiro, JSA, Eloy, LR Streit, DP Jr (2015) Seminal characteristics of piabanha before and after induction with different hormones. Acta Sci Biol Sci 37, 399–403.Google Scholar
Hafez, E.S.E Hafez, B (2004) Reprodução Animal, 7th edn, Manole: Barueri, 509 pp.Google Scholar
Herman, HA, Mitchell, JR Doak, GA (1994) The Artificial Insemination and Embryo Transfer of Dairy and Beef Cattle. Danville, Illinois, USA: Interstate Publishers, 392 pp.Google Scholar
Kavamoto, ET, Barnabe, VH, Campos, BES Andrade-Talmelli, EF (1999) Anormalidades morfológicas nos espermatozoides do curimbatá, Prochilodus scrofa (Steindachner, 1881) (Osteichthyes, Characiformes, Prochilodontidae). Bol Inst Pesca 25, 61–66.Google Scholar
Kime, DE Nash, JP (1999) Gamete viability as an indicator of reproductive endocrine disruption in fish. Sci Total Environ 233, 123–129.Google Scholar
Lahnsteiner, F, Berger, B, Weismann, T Patzner, RA (1998) Determination of semen quality of the rainbow trout, Oncorhynchus mykiss, by sperm motility, seminal plasma parameters, and spermatozoal metabolism. Aquaculture 163, 163–181.Google Scholar
Lima, FCT (2003) Subfamily Bryconinae (Characins, Tetras). In Check List of the Freshwater Fishes of South and Central America (eds RE Reis, SO Kullander and CJ Ferraris Jr, pp. 174–181. Porto Alegre: EDPURCS.Google Scholar
Maria, AN, Azevedo, HC, Santos, JP Carneiro, PCF (2012) Hormonal induction and semen characteristics of tambaqui Colossoma macropomum . Zygote 20, 39–43.Google Scholar
Martins, EDFF, Streit, DP Jr, Abreu, JSD, Correa-Filho, RAC, Oliveira, CALD, Lopera-Barrero, NM Povh, JA (2017) Ovopel and carp pituitary extract for the reproductive induction of Colossoma macropomum males. Theriogenology 98, 57–61.Google Scholar
Miliorini, AB, Murgas, LDS, Rosa, PV, Oberlender, G, Pereira, GJM Costa, DV (2011) A morphological classification proposal for curimba (Prochilodus lineatus) sperm damages after cryopreservation. Aquac Res 42, 177–187.Google Scholar
Moraes, GV, Streit, DP Jr, Ribeiro, RP, Sakaguti, ES, Souza, ED Povh, J A (2004) Ação de diferentes indutores hormonais no aparecimento de anormalidades morfológicas em espermatozoides de piavuçú (Leporinus macrocephalus), curimbatá (Prochilodus lineatus) e carpa comum (Cyprinus carpio). Bol Inst Pesca 30, 109–116.Google Scholar
Mousavi, SE, Yousefian, M, Masoudnia, A Fahimi, S (2011) Effects of three different GnRH analogues in combination with two different dopamine antagonists on milt production of endangered non-spermiating male caspian brown trout, Salmo trutta caspius . Ad Environ Biol 5, 2761–2768.Google Scholar
Murgas, LDS, Felizardo, VO, Ferreira, MR, Andrade, ES Veras, GC (2011) Importância da avaliação dos parâmetros reprodutivos em peixes nativos. Rev Bras Reprod An 35, 186–191.Google Scholar
Nakaghi, L, Neumann, E, Faustino, F, Mendes, J De Braga, F (2014) Moments of induced spawning and embryonic development of Brycon amazonicus (Teleostei, Characidae). Zygote 22, 549–557.Google Scholar
Ninhaus-Silveira, A, Foresti, F, Veríssimo-Silveira, R Senhorini, JA (2006) Seminal analysis, cryogenic preservation, and fertility in matrinxã fish, Brycon cephalus (Günther, 1869). Brazil Arch Biol Technol 49, 651–659.Google Scholar
Pardo-Carrasco, SC, Suárez-mahecha, H, Munõz-Lara, D, Arias-Castellanos, JA Gil, H (2002) Inducción de la ovulación y del desove del yamú, Brycon siebenthalae, con implantes de mGnRH-a. Bol Inst Pesca 28, 19–24.Google Scholar
Pardo-Carrasco, SC, Zaniboni-Filho, E, Arias-Castellanos, JA, Suárez-Mahecha, H, Atencio-García, VJ Cruz-Casallas, PE (2006) Evaluation of milt quality of the yamú Brycon amazonicus under hormonal induction. Rev Colom Cien Pecu 19, 134–139.Google Scholar
Ramos, RO, Ramos, SM Medonça, JOJ (1997) Utilização de análogos do LHRH, na indução do matrinchã Brycon cephalus Bol Téc . CEPTA 10, 1–7.Google Scholar
Ramos, SM (2000) Efeito de análogos de LHRH em combinação com receptores antagonistas de dopamina na indução à ovulação do matrinxã, Brycon cephalus (Günther, 1869). São Paulo: Dissertação (Mestrado em Zootecnia). Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo – USP, 48 pp.Google Scholar
Romagosa, E, Narahara, MY, Borella, MI Fenerich-Verani, N (2001) Seleção e caracterização de fêmeas de matrinxã, Brycon cephalus, induzidas a reprodução. Bol Inst Pesca 27, 139–147.Google Scholar
Rurangwa, E, Kime, DE, Ollevier, F Nash, JP (2004) The measurement of sperm motility and factors affecting sperm quality in cultured fish. Aquaculture 234, 1–28.Google Scholar
Schulz, RW Miura, T (2002) Spermatogenesis and its endocrine regulation. Fish Physiol Biochem 26, 43–56.Google Scholar
Streit, DP Jr (2002) Extrato de hipófise de frango e de coelho como indutores gonadais de pacu (Piaractus mesopotamicus) macho e fêmea, em comparação com extrato de carpa. Dissertação (Mestrado em Zootecnia). Centro de Ciências Agrárias. Maringá: Universidade Estadual de Maringá – UEM, 38 pp.Google Scholar
Streit, DP Jr, Benites, C, Moraes, GV, Ribeiro, RP, Sakaguti, ES Caldieri, RF (2006) Sêmen de pacu (Piaractus mesopotamicus) criopreservado com diluentes utilizados para sêmen de suínos. Ciên Anim Bras 7, 289–297.Google Scholar
Streit, DP Jr, Moraes, GV, Ribeiro, RP, Povh, JA, Souza, ED Oliveira, ACL (2004) Avaliação de diferentes técnicas para coloração de sêmen de peixes Arq Ciênc . Vet Zool UNIPAR 7, 157–162.Google Scholar
Streit, DP Jr, Sirol, R.N, Ribeiro, RP, Digmayer, M, Galo, JM, Moraes, GV Povh, JA (2012) Parâmetros seminais de reprodutores de Pseudoplatystoma reticulatum, em cativeiro, pré e pós-indução hormonal. Rev Bras Reprod An 36, 188–193.Google Scholar
Streit, DP Jr, Sirol, RN, Ribeiro, RP, Moraes, GV, Galo, JM Digmayer, M (2008) Parâmetros qualitativos do sêmen de dourado (Salminus maxillosus) em cativeiro. Bol Inst Pesca 34, 337–344.Google Scholar
Vidal, LVO, Furuya, WM, Graciano, TS, Schamber, CR, Silva, LCR, Santos, LD Souza, SR (2007) Eugenol como anestésico para juvenis de matrinxã (Brycon cephalus). Rev Bras Saúde e Prod Anim 8, 335–342.Google Scholar
Viveiros, ATM, Gonçalves, ACS, Di Chiacchio, IM, Nascimento, AF, Romagosa, E Leal, MC (2013) Gamete quality of streaked prochilod Prochilodus lineatus (Characiformes) after GnRHa and dopamine antagonist treatment. Zygote 23, 212–221.Google Scholar
Zaniboni-Filho, E Weingartner, M (2007) Técnicas de indução da reprodução de peixes migradores. Rev Bras Reprod An 31, 367–373.Google Scholar
Zohar, Y Mylonas, CC (2001) Endocrine manipulations of spawning in cultured fish: from hormones to genes. Aquaculture 197, 99–136.Google Scholar