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Sperm dilution ratio affects post-thaw motility rate and velocity of Prochilodus lineatus (Characiformes) sperm

Published online by Cambridge University Press:  18 December 2015

Ana T.M. Viveiros  and
Marcelo C. Leal
Ana T.M. Viveiros*
Affiliation:
Animal Science Department, Federal University of Lavras, P.O. Box 3037, Lavras, MG, 37200-000, Brazil.
Marcelo C. Leal
Affiliation:
Department of Animal Science, Federal University of Lavras, Minas Gerais, Brazil.
*
All correspondence to: Ana T.M. Viveiros. Animal Science Department, Federal University of Lavras, P.O. Box 3037, Lavras, MG, 37200-000, Brazil. Tel: +55 35 38291223. Fax: +55 35 38291231. E-mail address: anatmviveiros@outlook.com
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Summary

There is a lack of standardization in sperm cryopreservation of aquatic organisms and, thus, a necessity of more accurate investigations in all steps of this process. In this study, the effects of sperm dilution ratio on post-thaw sperm quality of Prochilodus lineatus were evaluated. Sperm was diluted in a standard freezing medium (glucose and methyl glycol) at four different ratios (sperm to final volume = 1:5, 1:10, 1:50 or 1:100), frozen in a nitrogen vapour vessel at –170°C and then stored in liquid nitrogen vessel at –196°C. Post-thaw motility rate and velocities (curvilinear = VCL; average path = VAP; straight line = VSL) were determined using a Computer-Assisted Sperm Analyzer (CASA) at 10 and 40 s post-activation. The highest motility rates were observed when sperm was frozen at a ratio of 1:5 (76%) and 1:10 (75%). The highest VCL (225 μm/s) and VAP (203 μm/s) were observed at a ratio of 1:10, while VSL was similar among samples frozen at 1:5, 1:10 and 1:50 (97–124 μm/s). When those parameters were evaluated again 30 s later, motility decreased significantly in samples frozen at a ratio of 1:5 (57%) and 1:10 (61%), while velocities decreased significantly in all samples regardless of dilution ratio (75–85 μm/s of VCL, 38–53 μm/s of VAP and 25–39 μm/s of VSL). P. lineatus sperm should be frozen at a ratio of 1:10, where both the number of loaded sperm per straw and the post-thaw quality are maximized.

Keywords

CASACryopreservationFishNeotropicalSemen
Type
Research Article
Information
Zygote , Volume 24 , Issue 5 , October 2016 , pp. 662 - 667
Copyright
Copyright © Cambridge University Press 2015 

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References

Cabrita, E., Robles, V., Cuñado, S., Wallace, J.C., Sarasquete, C. & Herráez, M.P. (2005). Evaluation of gilthead sea bream, Sparus aurata, sperm quality after cryopreservation in 5 ml macrotubes. Cryobiology 50, 273–84.CrossRefGoogle ScholarPubMed
Cabrita, E., Ma, S., Diogo, P., Martínez-Páramo, S., Sarasquete, C. & Dinis, M.T. (2011). The influence of certain amino acids and vitamins on post-thaw fish sperm motility, viability and DNA fragmentation. Anim. Reprod. Sci. 125, 189–95.CrossRefGoogle ScholarPubMed
Carolsfeld, J., Godinho, H.P., Zaniboni Filho, E. & Harvey, B.J. (2003). Cryopreservation of sperm in Brazilian migratory fish conservation. J. Fish Biol. 63, 472–89.CrossRefGoogle Scholar
Ciereszko, A., Dietrich, G.J., Nynca, J., Liszewska, E., Karol, H. & Dobosz, S. (2013). The use of concentrated extenders to improve the efficacy of cryopreservation in whitefish spermatozoa. Aquaculture 408–9, 30–3.CrossRefGoogle Scholar
Cordiviola de Yuan, E. (1992). Fish populations of lentic environments of the Parana River. Hydrobiologia 237, 159–73.CrossRefGoogle Scholar
Dong, Q., Huang, C. & Tiersch, T.R. (2007). Control of sperm concentration is necessary for standardization of sperm cryopreservation in aquatic species: evidence from sperm agglutination in oysters. Cryobiology 54, 87–98.CrossRefGoogle ScholarPubMed
Gonçalves, A.C.S., Nascimento, A.F., Costa, A.C., Leal, M.C. & Viveiros, A.T.M. (2013). Initiation and suppression of sperm motility is osmolality-dependent in two South American fish species: streaked prochilod (Prochilodus lineatus) and piracanjuba (Brycon orbignyanus). Anim. Reprod. 10, 62–70.Google Scholar
Kopeika, E. & Kopeika, J. (2008). Variability of sperm quality after cryopreservation in fish. In Fish spermatology (eds Alavi, S.M.H., Cosson, J., Coward, K. & Rafiee, G.), pp. 347–96. Alpha Science Intl Ltd, Oxford.Google Scholar
Lahnsteiner, F., Mansour, N. & Weismann, T. (2002). A new technique for insemination of large egg batches with cryopreserved semen in the rainbow trout. Aquaculture 209, 359–67.CrossRefGoogle Scholar
Orfão, L.H., Maria, A.N., Nascimento, A.F., Isaú, Z.A. & Viveiros, A.T.M. (2010). Sperm fertility of the subtropical freshwater streaked prochilod Prochilodus lineatus (Characiformes) improved after dilution and cold storage. Aquac. Res. 41, 679–87.Google Scholar
R Development Core Team. (2010). R: A Language and Environment for Statistical Computing. Version 2.12.0. R Foundation for Statistical Computing.Google Scholar
Rosenthal, H., Asturiano, J.F., Linhart, O. & Horvath, A. (2010). Foreword on the biology of fish gametes: summary and recommendations of the Second International Workshop, Valencia, Spain, 2009. J. Appl. Ichthyol. 26, 621–2.CrossRefGoogle Scholar
Suquet, M., Dreanno, C., Fauvel, C., Cosson, J. & Billard, R. (2000). Cryopreservation of sperm in marine fish. Aquac. Res. 31, 231–43.CrossRefGoogle Scholar
Van Zutphen, L.F.M., Baumans, V. & Beynen, A.C. (2001). Principles of Laboratory Animal Science. Revised edition. Amsterdam: Elsevier, 428 pp.Google Scholar
Viveiros, A.T.M. & Godinho, H.P. (2009). Sperm quality and cryopreservation of Brazilian freshwater fish species: a review. Fish. Physiol. Biochem. 35, 137–50.CrossRefGoogle ScholarPubMed
Viveiros, A.T.M., Orfão, L.H., Maria, N.A. & Allaman, I.B. (2009). A simple, inexpensive and successful freezing method for curimba Prochilodus lineatus (Characiformes) semen. Anim. Reprod. Sci. 112, 293–300.CrossRefGoogle ScholarPubMed
Viveiros, A.T.M., Nascimento, A.F., Orfão, L.H. & Isaú, Z.A. (2010). Motility and fertility of the subtropical freshwater fish streaked prochilod (Prochilodus lineatus) sperm cryopreserved in powdered coconut water. Theriogenology 74, 551–6.CrossRefGoogle ScholarPubMed
Viveiros, A.T.M., Gonçalves, A.C.S., Di Chiacchio, I.M., Nascimento, A.F., Romagosa, E. & Leal, M.C. (2015a). Gamete quality of streaked prochilod Prochilodus lineatus (Characiformes) after GnRHa and dopamine antagonist treatment. Zygote 23, 212–21.CrossRefGoogle ScholarPubMed
Viveiros, A.T.M., Nascimento, A.F., Leal, MC., Gonçalves, A.C.S., Orfão, L.H. & Cosson, J. (2015b). Methyl glycol, methanol and DMSO effects on post-thaw motility, velocities, membrane integrity and mitochondrial function of Brycon orbignyanus and Prochilodus lineatus (Characiformes) sperm. Fish. Physiol. Biochem. 41 (1), 193–201.CrossRefGoogle ScholarPubMed