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Strain difference in the timing of meiosis resumption in mouse oocytes: involvement of a cytoplasmic factor(s) acting presumably upstream of the dephosphorylation of p34cdc2 kinase

Published online by Cambridge University Press:  26 September 2008

Zbigniew Polanński
Zbigniew Polanński*
Affiliation:
Department of Genetics Evolution, Institute of Zoology, Jagiellonian University Krabów, Poland
*
Dapartment of Genetics and Evolution, Institute of Zoology, Jagiellonian University, Ingardena 6, 30-060 Kraków, Poland. Telephone: +48-12-336377 ext 434. Fax: +48-12-343716. e-mail: pola@zuk.iz.uj.edu.pl.
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Summary

Oocytes from eight inbred strains of mice were screened for the timing of germinal vesicle breakdown (GVB) in vitro. This characteristic varied between strains, reaching most extreme values in oocytes from AKR and BALB/c mice (3.1 and 1.6h after release from dibutyryl cAMP block, respectively; p<0.0001). The difference between AKR and BALB/c mice was confirmed in experiments in which GVB was induced in vivo by stimulation with exogenous gonadotrophins. Analysis of the rate of GVB in hybrids obtained after fusion of nuclear and cytoplamic fragments of oocytes from both strains suggests that the factor responsible for the difference between AKR and BALB/c mice is located in the cytoplasm of the proghase oocytes. Finally, in oocytes from both strains stimulated to resume meiotic maturation with okadaic acid, an inhibitor of protein phosphatases types 1 and 2A the rate of GVB was the same (2.2h and 2.3h for AKR and BALB/c, respectively; p= 0.48). This suggests that the difference between strains is not related to the amount or quality of the pre-MPF (Maturation Promoting Factor) stored in the prophase oocyte, but to the factor(s) acting upstream of the dephosphorylation of p34cdc2. kinase in the pathway leading to pre-MPF activation.

Keywords

Cytoplasmic factor(s)InterstraindifferencesMouse oocyteTiming of GVB
Type
Article
Information
Zygote , Volume 5 , Issue 2 , May 1997 , pp. 105 - 109
Copyright
Copyright © Cambridge University Press 1997

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