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Structural analysis of toad oviductal mucosa in relation to jelly components secretion throughout the reproductive cycle

Published online by Cambridge University Press:  22 November 2012

Claudia Alejandra Crespo ,
Inés Ramos ,
Marcela Fátima Medina ,
Susana Beatriz Cisint ,
Ana Lucrecia Iruzubieta Villagra  and
Silvia Nélida Fernández
Claudia Alejandra Crespo
Affiliation:
Superior Institute of Biological Research, National Council for Scientific and Technical Research, National University of Tucumán, Chacabuco 461, Tucumán 4000, Argentina.
Inés Ramos
Affiliation:
Superior Institute of Biological Research, National Council for Scientific and Technical Research, National University of Tucumán, Chacabuco 461, Tucumán 4000, Argentina.
Marcela Fátima Medina
Affiliation:
Superior Institute of Biological Research, National Council for Scientific and Technical Research, National University of Tucumán, Chacabuco 461, Tucumán 4000, Argentina.
Susana Beatriz Cisint
Affiliation:
Superior Institute of Biological Research, National Council for Scientific and Technical Research, National University of Tucumán, Chacabuco 461, Tucumán 4000, Argentina.
Ana Lucrecia Iruzubieta Villagra
Affiliation:
Superior Institute of Biological Research, National Council for Scientific and Technical Research, National University of Tucumán, Chacabuco 461, Tucumán 4000, Argentina.
Silvia Nélida Fernández*
Affiliation:
Superior Institute of Biological Research, National University of Tucumán, Chacabuco 461, Tucumán 4000, Argentina.
*
All correspondence to: Silvia N. Fernández. Superior Institute of Biological Research, National University of Tucumán, Chacabuco 461, Tucumán 4000, Argentina. Tel: +54 0381 424 7752/7005. Fax: +54 0381 424 7752/7044. e-mail: sfernandez@fbqf.unt.edu.ar
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Summary

In amphibians, the components of the jelly coats that surround the oocytes at the time of fertilization and coordinate gamete interaction are secreted by the oviduct. We analysed the histological variations in the mucosa of the oviductal pars convoluta (PC) of Rhinella arenarum during the reproductive cycle and its relationship with secretion. During the preovulatory period, the mucosa reaches a high degree of morphological and functional development, with a large number of epithelial (ESC) and glandular secretory cells (GSC) loaded with contents that are secreted into the oviductal lumen. During the ovulatory period, the secretory cells (SC) of both layers present maximum secretory activity through apocrinia and merocrinia. While the ESC located at the tips of the folds release their content directly in contact with the oocytes, the GSC secrete material from the bottom of the epithelial folds that, by interaction with the secretion of the ESC in the lateral faces, form a product with a certain degree of organization. Secretion is a continuous process with formation of coats of increasing complexity from the intermediate proximal zone (IPZ) to the pars convoluta (pc) itself, and the passage of the oocyte is a requisite for the organization of the jelly coats around the gamete.

During the early postovulatory period, although there is a marked decrease in the number and volume of the SC, the ESC still release material into the oviductal lumen. In the late postovulatory period the morphological characteristics of the PC begin to recovery although there is no evidence of secretion.

Keywords

AmphibianFertilizationJelly coatsOviductal secretionReproductive cycle
Type
Research Article
Information
Zygote , Volume 22 , Issue 2 , May 2014 , pp. 229 - 238
Copyright
Copyright © Cambridge University Press 2012 

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