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Supplementation of culture medium with knockout serum replacement improves the survival of bovine secondary follicles when compared with other protein sources during in vitro culture

Published online by Cambridge University Press:  11 October 2019

D.S. Gomes ,
L.B. Aragão ,
M.F. Lima Neto ,
P.A.A. Barroso ,
L.R.F.M. Paulino ,
B.R. Silva ,
A.L.P. Souza ,
G.L. Vasconcelos ,
A.W.B. Silva  and
J.R.V. Silva Open the ORCID record for J.R.V. Silva [Opens in a new window]
D.S. Gomes
Affiliation:
Postgraduate Programme in Biotechnology, Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceara, Campus of Sobral, Sobral, CE, Brazil
L.B. Aragão
Affiliation:
Postgraduate Programme in Biotechnology, Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceara, Campus of Sobral, Sobral, CE, Brazil
M.F. Lima Neto
Affiliation:
Postgraduate Programme in Biotechnology, Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceara, Campus of Sobral, Sobral, CE, Brazil
P.A.A. Barroso
Affiliation:
Postgraduate Programme in Biotechnology, Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceara, Campus of Sobral, Sobral, CE, Brazil
L.R.F.M. Paulino
Affiliation:
Postgraduate Programme in Biotechnology, Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceara, Campus of Sobral, Sobral, CE, Brazil
B.R. Silva
Affiliation:
Postgraduate Programme in Biotechnology, Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceara, Campus of Sobral, Sobral, CE, Brazil
A.L.P. Souza
Affiliation:
Postgraduate Programme in Biotechnology, Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceara, Campus of Sobral, Sobral, CE, Brazil
G.L. Vasconcelos
Affiliation:
Postgraduate Programme in Biotechnology, Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceara, Campus of Sobral, Sobral, CE, Brazil
A.W.B. Silva
Affiliation:
Postgraduate Programme in Biotechnology, Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceara, Campus of Sobral, Sobral, CE, Brazil
J.R.V. Silva*
Affiliation:
Postgraduate Programme in Biotechnology, Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceara, Campus of Sobral, Sobral, CE, Brazil
*
Author for correspondence: JRV Silva. Federal University of Ceara, Av. Comandante Maurocélio Rocha Ponte 100, CEP 62041-040, Sobral, CE, Brazil. Tel:/Fax: +55 88 36118000. E-mail: jrvsilva@ufc.br
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Summary

The present study evaluated the effect of knockout serum replacement (KSR), fetal bovine serum (FBS) and bovine serum albumin (BSA) on the viability and growth of bovine secondary follicles cultured in vitro for 12 days. To this end, secondary follicles were isolated (185–202 μm) and cultured in vitro in TCM-199+ medium supplemented with KSR (5% and 10%), FBS (5% and 10%) or BSA (3 mg/ml) at 38.5°C with 5% CO2 in air. Follicular diameters were evaluated on days 0, 4, 8 and 12. After 12 days of culture, follicular survival analysis was performing by using calcein-AM and ethidium homodimer. Before and after culture, follicles were fixed in paraformaldehyde for histological evaluation. Follicular diameter at different days of culture were compared using the Kruskal–Wallis test, while the percentages of viable follicles were analyzed by chi-squared test (P < 0.05). Results showed that follicles cultured in the presence of KSR at both concentrations presented higher follicular survival rates than those cultured in control medium alone or supplemented with FBS or BSA. Conversely, the presence of KSR, BSA or FBS did not increase follicular diameter after 12 days of culture. Histology analysis showed that, among the tested treatments, follicles cultured in the presence of KSR had preserved rounded oocytes, juxtaposed granulosa cells and intact basal membrane. In conclusion, supplementation of culture medium with KSR increases the follicular survival of bovine secondary follicles cultured in vitro.

Keywords

CowIn vitro cultureKnockout serum replacementOocytesSecondary follicles
Type
Research Article
Information
Zygote , Volume 28 , Issue 1 , February 2020 , pp. 32 - 36
Copyright
© Cambridge University Press 2019 

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