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Time course of the meiotic arrest in sheep cumulus–oocyte complexes treated with roscovitine

Published online by Cambridge University Press:  14 July 2015

Letícia Ferrari Crocomo ,
Wolff Camargo Marques Filho ,
Camila Louise Ackermann ,
Daniela Martins Paschoal ,
Midyan Daroz Guastali ,
Rosiára Rosária Dias Maziero ,
Mateus José Sudano ,
Fernanda da Cruz Landim-Alvarenga  and
Sony Dimas Bicudo
Letícia Ferrari Crocomo*
Affiliation:
Rua dos Girassóis, n.278 – Bairro Convívio – CEP: 18605–240 Botucatu – S.P., Brazil. Department of Animal Reproduction and Veterinary Radiology - School of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP) - Botucatu, Brazil.
Wolff Camargo Marques Filho
Affiliation:
Department of Animal Reproduction and Veterinary Radiology - School of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP) - Botucatu, Brazil.
Camila Louise Ackermann
Affiliation:
Department of Animal Reproduction and Veterinary Radiology - School of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP) - Botucatu, Brazil.
Daniela Martins Paschoal
Affiliation:
Department of Animal Reproduction and Veterinary Radiology - School of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP) - Botucatu, Brazil.
Midyan Daroz Guastali
Affiliation:
Department of Animal Reproduction and Veterinary Radiology - School of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP) - Botucatu, Brazil.
Rosiára Rosária Dias Maziero
Affiliation:
Department of Animal Reproduction and Veterinary Radiology - School of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP) - Botucatu, Brazil.
Mateus José Sudano
Affiliation:
Laboratory of Genetics and Animal Breeding, Federal University of Pampa, Uruguaiana, RS, Brazil.
Fernanda da Cruz Landim-Alvarenga
Affiliation:
Department of Animal Reproduction and Veterinary Radiology - School of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP) - Botucatu, Brazil.
Sony Dimas Bicudo
Affiliation:
Department of Animal Reproduction and Veterinary Radiology - School of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP) - Botucatu, Brazil.
*
All correspondence to: Letícia Ferrari Crocomo. Rua dos Girassóis, n.278 – Bairro Convívio – CEP: 18605–240 Botucatu – S.P., Brazil. Tel: + 55 14 3882–4743. E-mail: leticia.crocomo@gmail.com
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Summary

Temporary meiosis arrest with cyclin-dependent kinases inhibitors has been proposed in order to improve the quality of in vitro matured oocytes. In sheep, however, this phenomenon has been rarely investigated. Therefore, the present study aimed to evaluate the effect of different incubation times with roscovitine on nuclear maturation and cumulus cell expansion of sheep cumulus–oocyte complexes (COCs). For this, COCs were cultured for 0, 6, 12 or 20 h in basic maturation medium (Control) containing 75 μM roscovitine (Rosco). After, they were in vitro matured (IVM) for 18 h in the presence of luteinizing hormone (LH) and follicle-stimulating hormone (FSH). At the end of each treatment, cumulus cell expansion and nuclear maturation were assessed under a stereomicroscope and by Hoechst 33342 staining, respectively. In the Control and Rosco groups, the absence of cumulus cell expansion prevailed at 0, 6, 12 and 20 h. After IVM for 18 h, total cumulus cell expansion in the Rosco treatments was dependent on the exposure time to roscovitine. A significantly high percentage of oocytes treated with roscovitine for 6 h (87%), 12 h or 20 h (65%) were arrested at the germinal vesicle (GV) stage. In contrast, 23% GVBD, 54% metaphase I (MI) and 61% MII oocytes were observed in the Control groups at 6, 12 and 20 h, respectively. In all treatments, a significant percentage of oocytes reached MII after IVM for 18 h. Therefore, roscovitine reversibly arrested the meiosis of sheep oocytes during different culture times with the maximal efficiency of meiotic inhibition reached at 6 h. In addition, reversibility of its inhibitory action on cumulus cells was exposure-time dependent.

Keywords

Cumulus cell expansionInhibitorMeiosisNuclear maturationOvine
Type
Research Article
Information
Zygote , Volume 24 , Issue 2 , April 2016 , pp. 310 - 318
Copyright
Copyright © Cambridge University Press 2015 

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