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Toxicity of cryoprotectants agents in freshwater prawn embryos of Macrobrachium amazonicum

Published online by Cambridge University Press:  26 September 2014

Arthur Vinícius Lourenço Ferreira ,
Elias José Teles Castro ,
Mariana Silva Alves Barbosa ,
Míriam Luzia Nogueira Martins de Sousa ,
Manoel Paiva de Araújo Neto ,
Aldeney Andrade Soares Filho  and
Celia Maria de Souza Sampaio
Arthur Vinícius Lourenço Ferreira*
Affiliation:
Dept of Biology, State University of Ceara, UECE, Fortaleza, CE, 60740–000, Brazil.
Elias José Teles Castro
Affiliation:
Dept of Biology, State University of Ceará, UECE, Fortaleza, CE, Brazil.
Mariana Silva Alves Barbosa
Affiliation:
Dept of Biology, State University of Ceará, UECE, Fortaleza, CE, Brazil.
Míriam Luzia Nogueira Martins de Sousa
Affiliation:
Postgraduate Program in Veterinary Science, UECE, Fortaleza, CE, Brazil.
Manoel Paiva de Araújo Neto
Affiliation:
Laboratory of Ecology of Mangroves, Federal Institute of Education, Science and Technology of Ceará, IFCE, Acaraú, CE, Brazil.
Aldeney Andrade Soares Filho
Affiliation:
Dept of Fishing Engineering, Federal University of Ceará, UFC, Fortaleza, CE, Brazil.
Celia Maria de Souza Sampaio
Affiliation:
Dept of Biology, State University of Ceará, UECE, Fortaleza, CE, Brazil.
*
All correspondence to: Arthur Vinícius Lourenço Ferreira. Dept of Biology, State University of Ceara, UECE, Fortaleza, CE, 60740–000, Brazil. Tel:/Fax: +85 3101 9927. E-mail: arthurvinicius.lf@gmail.com
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Summary

The process of cooling and cryopreservation of prawn embryos is a viable alternative for a continuous supply of larvae for freshwater prawn farming ponds. However, studies involving the application of those techniques as well as on toxicity of cryoprotectants in freshwater prawn embryos are scarce. Thus, this study aims to test the toxicity of methylic alcohol (MET), dimethyl sulfoxide (DMSO) and ethylene glycol (EG) on Macrobrachium amazonicum embryos. For the present experiment, pools of embryos were taken from 15 M. amazonicum females and were divided into three groups and tested in duplicate at concentrations of 10, 5, 3; 1, 0.5 or 0.1%. Toxicity tests were conducted for 24 h in Falcon® pipes to obtain the lethal concentration for 50% of the larvae (LC50). After the set period for testing, random samples of embryos were removed for morphological analysis under stereoscopic microscopes. Results were analysed using analysis of variance (ANOVA) and Tukey's test at a 5% significance level and Trimmed Spearman-Karber Analysis to determine LC50-24 h. DMSO toxicity tests revealed that 5% and 10% concentrations showed the highest toxicity and differed from the control (P ≤ 0.05), 24h-LC50 was 437.4 ± 14.4 µL. MET was less toxic among the tested cryoprotectants and concentrations did not allow the determination of its LC50-24h. For tests with EG, concentrations of 3, 5 or 10% solutions resulted in a 100% mortality to tested embryos; EG was the tested cryoprotectant with the highest toxicity, with an LC50-24h average of 81.91 ± 35.3 µl.

Keywords

Amazon River prawnDimethyl sulfoxideEthylene glycolLC50; Methylic alcohol
Type
Research Article
Information
Zygote , Volume 23 , Issue 6 , December 2015 , pp. 813 - 820
Copyright
Copyright © Cambridge University Press 2014 

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