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Ultraviolet radiation and handling medium osmolarity affect chimaerism success in zebrafish

Published online by Cambridge University Press:  23 June 2010

M. Francisco-Simão ,
J. Cardona-Costa ,
M. Pérez-Camps  and
F. García-Ximénez
M. Francisco-Simão
Affiliation:
Laboratory of Animal Reproduction and Biotechnology (LARB-UPV), Universidad Politécnica de Valencia, Camino de Vera 14,46071 Valencia, Spain. Faculty of Agriculture Sciences – Agostinho Neto University, Angola.
J. Cardona-Costa*
Affiliation:
Laboratory of Animal Reproduction and Biotechnology (LARB-UPV), Universidad Politécnica de Valencia, Camino de Vera 14,46071 Valencia, Spain.
M. Pérez-Camps
Affiliation:
Laboratory of Animal Reproduction and Biotechnology (LARB-UPV), Universidad Politécnica de Valencia, Camino de Vera 14,46071 Valencia, Spain.
F. García-Ximénez
Affiliation:
Laboratory of Animal Reproduction and Biotechnology (LARB-UPV), Universidad Politécnica de Valencia, Camino de Vera 14,46071 Valencia, Spain.
*
All correspondence to: J. Cardona-Costa. Laboratory of Animal Reproduction and Biotechnology (LARB-UPV), Universidad Politécnica de Valencia, Camino de Vera 14,46071 Valencia, Spain. Tel: +34 963879433. Fax: +34 963877439. e-mail: cardona_costa_j@hotmail.com
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Summary

The effects of a predefined ultraviolet radiation dose (0.529 mW/cm2 for 30s) together with two different micromanipulation medium osmolarities (30 mOsm/kg vs 300 mOsm/kg) were tested on embryo survival at different developmental stages and on the somatic (skin) and germ-line chimaerism rates. Somatic (13%, 6/47 adults) and germ-line chimaerism (50% pigmented F1 larvae) were detected only in the UV-treated recipient embryos micromanipulated in a 300 mOsm/kg medium. From the results obtained, we concluded that the conditions cited above were the most suitable to improve somatic and germ-line chimaerism rates in zebrafish.

Keywords

EmbryoGerm-line chimaerismOsmolarityZebrafish
Type
Research Article
Information
Zygote , Volume 19 , Issue 1 , February 2011 , pp. 67 - 70
Copyright
Copyright © Cambridge University Press 2010

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References

Cardona-Costa, J. & García-Ximénez, F. (2007). Vitrification of zebrafish embryo blastomeres in microvolumes. Cryoletters 28, 303–9.Google ScholarPubMed
Cardona-Costa, J., Francisco-Simão, M., Pérez-Camps, M. & García-Ximénez, F. (2009). Micromanipulation medium osmolarity compromises zebrafish embryo and cell survival in chimaerism experiments. Zygote [Epub ahead of print].Google Scholar
Carsience, R.S., Mary, E.C., Ann, M., Verrinder, G. & Robert, J.E. (1993). Germline chimeric chickens from dispersed donor blastodermal cells and compromised recipient embryos. Development 117, 669–75.CrossRefGoogle ScholarPubMed
Fan, L., Alestrom, A. & Collodi, P. (2004). Production of zebrafish germline chimeras from cultured cells. Methods Mol. Biol. 254, 289–300.Google ScholarPubMed
Francisco-Simão, M., Cardona-Costa, J., Pérez-Camps, M. & García-Ximénez, F. (2009). Ultraviolet radiation dose to be applied in recipient zebrafish embryos for germ-line chimaerism is strain dependent. Reprod. Dom. Anim. [Epub ahead of print].Google Scholar
Joly, J.S., Kress, C., Vandeputte, M., Bourrat, F. & Chourrout, D. (1999). Irradiation of fish embryos prior to blastomere transfer boosts the colonisation of their gonads by donor–derived gametes. Mol. Reprod. Dev. 53, 394–7.3.0.CO;2-X>CrossRefGoogle ScholarPubMed
Li, Z.D., Deng, H., Liu, C.H., Song, Y.H., Sha, J., Wang, N. & Wei, H. (2002). Production of duck-chicken chimera by transferring early blastodermal cells. Poult. Sci. 81, 1360–4.CrossRefGoogle ScholarPubMed
Lin, S., Long, W., Chen, J. & Hokins, N. (1992). Production of germ-line chimeras in zebrafish by cell transplants from genetically pigmented to albino embryos. Proc. Natl. Acad. Sci. USA 89, 4519–23.CrossRefGoogle ScholarPubMed
Ma, C., Fan, L., Ganassin, R., Bols, N. & Collodi, P. (2001). Production of zebrafish germ-line chimeras from embryo cell cultures. Proc. Natl. Acad. Sci. 98, 2461–6.CrossRefGoogle ScholarPubMed
Nakagawa, M. & Ueno, K. (2003). Production of chimeric loach by cell transplantation from genetically pigmented to orange embryos. Zool. Sci. 20, 333–8.CrossRefGoogle ScholarPubMed
Pérez-Camps, M. & García-Ximénez, F. (2008). Osmolarity and composition of cell culture media affect further development and survival in zebrafish embryos. Animal 2, 595–9.CrossRefGoogle ScholarPubMed
Saito, T., Goto-Kazeto, R., Arai, K. & Yamaha, E. (2007). Xenogenesis in teleost fish through generation of germ-line chimaeras by single primordial germ cell transplantation. Biol. Reprod. 78, 159–66.CrossRefGoogle ScholarPubMed
Swartz, W.J. (1980). Response of early chick embryo to busulfan. Teratology 21, 1–8.CrossRefGoogle ScholarPubMed