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Unprotected freezing of human spermatozoa exerts a detrimental effect on their oocyte activating capacity and chromosome integrity

Published online by Cambridge University Press:  26 September 2008

Andrei V. Rybouchkin ,
Paul De Sutter  and
Marc Dhont
Andrei V. Rybouchkin*
Affiliation:
Infertility Centre, Department of Gynaecology and Obstetrics, University Hospital of Ghent, Ghent, Belgium.
Paul De Sutter
Affiliation:
Infertility Centre, Department of Gynaecology and Obstetrics, University Hospital of Ghent, Ghent, Belgium.
Marc Dhont
Affiliation:
Infertility Centre, Department of Gynaecology and Obstetrics, University Hospital of Ghent, Ghent, Belgium.
*
Andrei V. Rybouchkin, Infertility Centre, Department of Gynaecology and Obstetrics, University Hospital, De Pinetelaan 185, B-9000 Ghent, Belgium. Telephone +32 9 2402194. Fax: +32 9 240 4972. e-mail: andrei.rybouchin@rug.ac.be.
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Summary

The influence of unprotected freezing of mammalian spermatozoa on their oocyte activating capacity and chromosome integrity is unknown. However, this type of sperm treatment has been used in assisted reproduction by intracytoplasmic sperm injection in cattle and humans. The mouse oocyte injection test was used to analyse the influence of unprotected freezing of human spermatozoa on their reproductive characteristics. Mouse oocytes were microinjected with intact human spermatozoa or spermatozoa treated with two cycles of unprotected freeze-thawing. Oocytes surviving the injection were either cultured without further treatment or exposed to ethanol solution to induce parthenogenetic activation. Both injected and activated oocytes were used for sperm chromosome analysis. The results revealed a significant reduction in oocyte activating capacity and a tenfold increase in the incidence of structural chromosomal abnormalities in human spermatozoa treated by unprotected freezing. We conclude that unprotected freezing of human spermatozoa has a detrimental effect on their reproductive characteristics. Our data also provide a new perspective on the stability of mammalian spermatozoa to physical factors and demonstrate the importance of detailed analysis of the stability of sperm structures for successful development of new approaches in assisted reproduction.

Keywords

Intracytoplasmic sperm injectionOocyte activationSperm karyotypingUnprotected sperm freezing
Type
Article
Information
Zygote , Volume 4 , Issue 4 , November 1996 , pp. 263 - 268
Copyright
Copyright © Cambridge University Press 1996

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