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Value of transferring embryos derived from monopronucleated (1PN) zygotes at the time of fertilization assessment

Published online by Cambridge University Press:  20 March 2020

Ming Li Open the ORCID record for Ming Li [Opens in a new window] ,
Yujiao Dang ,
Ying Wang ,
Junsheng Li  and
Ping Liu
Ming Li*
Affiliation:
Center for Reproductive Medical, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing10091, China Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing10091, China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction Technology, Beijing100191, China National Clinical Research Center for Obstetrics and Gynecology, Beijing10091, China
Yujiao Dang
Affiliation:
Center for Reproductive Medical, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing10091, China Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing10091, China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction Technology, Beijing100191, China National Clinical Research Center for Obstetrics and Gynecology, Beijing10091, China
Ying Wang
Affiliation:
Center for Reproductive Medical, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing10091, China Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing10091, China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction Technology, Beijing100191, China National Clinical Research Center for Obstetrics and Gynecology, Beijing10091, China
Junsheng Li
Affiliation:
Center for Reproductive Medical, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing10091, China Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing10091, China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction Technology, Beijing100191, China National Clinical Research Center for Obstetrics and Gynecology, Beijing10091, China
Ping Liu
Affiliation:
Center for Reproductive Medical, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing10091, China Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing10091, China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction Technology, Beijing100191, China National Clinical Research Center for Obstetrics and Gynecology, Beijing10091, China
*
Author for correspondence: Ming Li, Department of Obstetrics and Gynecology, Reproductive Medical Center, Peking University Third Hospital, No. 49 North Huayuan Road, Haidian District, Beijing100191, China. Tel: +86 10 82266849. E-mail: bysylm@sina.com
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Summary

This paper is a retrospective analysis of the sole transfer of monopronucleated zygotes (1PN) embryos both in in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) to determine the value of transferring embryos formed from 1PN. In fresh cycles, 1PN cleavage-stage embryos (1PN cleavage fresh) were transferred. In frozen–thawed cycles, 1PN blastocyst-stage embryos (1PN blast frozen) were transferred. We used comparison groups: for fresh cycles, 2PN cleavage-stage embryos (2PN cleavage fresh) were transferred; and for frozen–thawed cycles, 2PN blastocyst-stage embryos (2PN blast frozen) were transferred. Comparison groups were matched for cycle and patient characteristics to the 1PN group. Finally, for fresh cycles, live birth rates (LBR) in the 1PN cleavage group were significantly lower than those in 2PN cleavage group, both for IVF [LBR = 7.64% vs. pregnancy rate (PR) = 22.12%, P = 0.003, respectively] and ICSI (LBR = 0% vs. LBR = 20.00%, P < 0.001, respectively). For frozen–thawed IVF cycles, the PR in the 1PN blastocyst group were comparable with those of the 2PN blastocyst group (1PN: LBR = 33.14% vs. 2PN: LBR = 37.24%, P = 0.289, respectively), while in ICSI, the PR in the 1PN blastocyst group were lower than those in the 2PN blastocyst group (LBR = 15.25% vs. LBR = 40.68%, P = 0.002, respectively). So, for IVF, blastocyst culture was capable of selecting normal 1PN embryos for transfer and achieves satisfying outcomes. However, for ICSI, blastocyst culture was not effective enough to eliminate abnormal embryos and 1PN embryo transfer needed to be treated with caution.

Keywords

1PNBlastocyst cultureEmbryo transferImplantation rateLive birth
Type
Research Article
Information
Zygote , Volume 28 , Issue 3 , June 2020 , pp. 241 - 246
Copyright
© Cambridge University Press 2020

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