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Visualization of early embryos of the butterfly Bicyclus anynana

Published online by Cambridge University Press:  20 July 2005

Jarod Masci  and
Antónia Monteiro
Jarod Masci
Affiliation:
Department of Biological Sciences, State University of New York, Buffalo, NY 14260, USA
Antónia Monteiro
Affiliation:
Department of Biological Sciences, State University of New York, Buffalo, NY 14260, USA
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Abstract

We report on the first attempts, using both light and fluorescence microscopy, to visualize the developing embryo of the butterfly Bicyclus anynana. We developed a new protocol that enabled the clear visualization of the internal egg structures in early embryogenesis (1–24 h after egg laying). Dechorionation was followed by fixation and physical dissection of the external egg structures. Observations of embryonic and extra-embryonic cells were made using a Hoechst nuclear stain that fluoresces in the blue spectrum when bound to DNA and excited with ultraviolet (UV) light under a fluorescence microscope. Preliminary data on the developmental rate of the early embryo are also presented.

Keywords

Bicyclus anynanaDevelopmentEmbryoEmbryogenesisLepidoptera
Type
Research Article
Information
Zygote , Volume 13 , Issue 2 , May 2005 , pp. 139 - 144
Copyright
2005 Cambridge University Press

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