Genetic diversity among mycelial compatibility groups of Sclerotium rolfsii (teleomorph Athelia rolfsii) and S. delphinii

Abstract

The genetic relationships among 132 isolates of Sclerotium rolfsii (teleomorph Athelia rolfsii) collected during 1967–97 from 36 different host species over a wide geographic range representing 13 countries were investigated using mycelial compatibility groupings and RAPD analysis. A smaller group of 15 Sclerotium delphinii isolates from five host species and a limited geographic distribution was also studied. The development of aversion reactions following mycelial pairings of isolates in all possible combinations on potato dextrose agar was used to differentiate 71 mycelial compatibility groups (MCG) in S. rolfsii and five MCG in S. delphinii. Many MCG were unique single-member groups and these generally were found in widely separated geographic regions or countries. There was no clear relationship between host of origin and MCG, except for a majority of isolates of S. rolfsii from turfgrass that belonged to MCG 1. Within a specific geographic region, e.g. California, there usually were several different MCG present, some of which were recovered from the same host species. In addition, specific MCG of S. rolfsii were recovered from widely separated geographic regions as well as different host species, e.g. MCG 1 was recovered from turfgrass, carrot, tobacco, and tomato in California, Georgia, North Carolina, and Mexico, respectively. The extent of genetic diversity within and among MCG of S. rolfsii and S. delphinii was studied using RAPD analysis. Isolates from different MCG could be differentiated by their unique banding patterns using six primers. There were no discernible relationships among the various MCG using UPGMA analysis. Isolates within a particular MCG were also genetically diverse, but shared greater numbers of common bands and clustered together. Only a few members of some MCG in S. rolfsii and S. delphinii that had identical RAPD patterns were considered to be clonally derived. The extent of genetic diversity among isolates of S. delphinii was lower than that observed in S. rolfsii.