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Immunohistochemical localisation of aquaporin 2 and vasopressin type 2 receptor in the human endolymphatic sac

Published online by Cambridge University Press:  12 December 2022

X Pan ,
C Huang ,
A Peng  and
Z Zhang Open the ORCID record for Z Zhang [Opens in a new window]
X Pan
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Second Xiangya Hospital, Central South University, Changsha, Hunan, China
C Huang
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Second Xiangya Hospital, Central South University, Changsha, Hunan, China
A Peng
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Second Xiangya Hospital, Central South University, Changsha, Hunan, China
Z Zhang*
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Second Xiangya Hospital, Central South University, Changsha, Hunan, China
*
Corresponding author: Zhiwen Zhang; Email: zhangzhiwen@csu.edu.cn
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Abstract

Objective

This study aimed to determine the distribution and subcellular localisation of aquaporin 2 and vasopressin type 2 receptor in the human endolymphatic sac.

Methods

Ten samples of human endolymphatic sac were collected during acoustic neurinoma removal using the translabyrinthine approach. Immunohistochemistry and immunofluorescence were performed using aquaporin 2 and vasopressin type 2 receptor monoclonal antibodies.

Results

Confocal microscopy demonstrated that vasopressin type 2 receptor labelling was expressed in both the apical and basolateral plasma membranes, and in the cytoplasm of the endolymphatic sac epithelium, whereas aquaporin 2 was strongly expressed at the basolateral site of the endolymphatic sac epithelium, in both the intraosseous and extraosseous parts of the endolymphatic sac.

Conclusion

Both aquaporin 2 and vasopressin type 2 receptor were detected in the epithelial cells of the human endolymphatic sac, suggesting that this channel may be involved in inner-ear fluid homeostasis. However, strong basolateral expression of aquaporin 2 in endolymphatic sac epithelium suggested that the function of aquaporin 2 may differ between the endolymphatic sac and kidney.

Keywords

Endolymphatic sacaquaporin 2immunohistochemistry
Type
Main Article
Information
The Journal of Laryngology & Otology , Volume 137 , Issue 12 , December 2023 , pp. 1340 - 1344
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of J.L.O. (1984) LIMITED

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Footnotes

Dr Z Zhang takes responsibility for the integrity of the content of the paper

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