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Alteration in microbes changed the contents of oviposition-deterrent pheromones on the Spodoptera litura egg surface

Published online by Cambridge University Press:  25 November 2024

Liming Hu ,
Yirui Chen ,
Qingjun Wu ,
Qiumei Zeng ,
Taoli Zhang ,
Guohui Yu ,
Muyang He ,
Dasong Chen ,
Xiangning Su  and
Yuping Zhang
...Show all authors
Liming Hu
Affiliation:
Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Zhongkai University of Agriculture and Engineering, Guangzhou, China
Yirui Chen
Affiliation:
Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Zhongkai University of Agriculture and Engineering, Guangzhou, China
Qingjun Wu
Affiliation:
State Key Laboratory of Vegetable Biobreeding, Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China
Qiumei Zeng
Affiliation:
Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Zhongkai University of Agriculture and Engineering, Guangzhou, China
Taoli Zhang
Affiliation:
Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Zhongkai University of Agriculture and Engineering, Guangzhou, China
Guohui Yu
Affiliation:
Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Zhongkai University of Agriculture and Engineering, Guangzhou, China
Muyang He
Affiliation:
Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Zhongkai University of Agriculture and Engineering, Guangzhou, China
Dasong Chen
Affiliation:
Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou, China
Xiangning Su
Affiliation:
Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences & Key Laboratory of Green Prevention and Control of Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs & Key Laboratory of High Technology for Plant Protection of Guangdong Province, Guangzhou, China
Yuping Zhang
Affiliation:
Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences & Key Laboratory of Green Prevention and Control of Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs & Key Laboratory of High Technology for Plant Protection of Guangdong Province, Guangzhou, China
Zhenfei Zhang
Affiliation:
Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences & Key Laboratory of Green Prevention and Control of Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs & Key Laboratory of High Technology for Plant Protection of Guangdong Province, Guangzhou, China
Jianmei Shen*
Affiliation:
Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Zhongkai University of Agriculture and Engineering, Guangzhou, China
*
Corresponding author: Jianmei Shen; Email: shenjianmei@zhku.edu.cn
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Abstract

Microorganisms symbiotic with insects, whether permanently or temporarily, play a crucial role in the nutrition, development, reproduction, defence, and metamorphosis regulation. In some Lepidoptera, oviposition-deterrent pheromones (ODPs) on egg surface were used by pregnant females to modify the behaviour of conspecifics to avoid excessive competition for limited resources. In this study, we constructed four different Spodoptera litura groups, including, OH, OA, SH, and OA, which either feed on different hosts or grow in different environments. The 16S rDNA libraries of microbes from the egg surface of the four groups were constructed and sequenced. According to alpha and beta diversity indices, the microbes in environments and diets considerably influenced the richness, diversity, and community compositions of the microbiota on egg surfaces. The quantity of the main ODP components and the corresponding oviposition-deterrent activity among four groups were significantly differed among the four groups. The result of this study revealed that altering of microbes in environments or diets considerably changed the contents of ODP and oviposition-deterrent activity. As ODPs impart oviposition-deterrent activity towards closely related species, the findings of this study suggest that we should pay more attention to the role of symbiotic microorganisms in changing the ability of insects, especially sympatric species, to occupy the optimal niche when developing novel pest-control strategies.

Keywords

16S rDNA sequencingegg surfacemicroorganisms symbioticoviposition-deterrent pheromonesSpodoptera litura
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 114 , Issue 6 , December 2024 , pp. 828 - 835
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Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press

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Footnotes

*

Joint corresponding authors.

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