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HSP gene superfamily in Aspongopus chinensis Dallas: unravelling identification, characterisation and expression patterns during diapause and non-diapause stages

Published online by Cambridge University Press:  01 March 2024

Xinyi Ma ,
Zhiyong Yin ,
Haiyin Li  and
Jianjun Guo Open the ORCID record for Jianjun Guo [Opens in a new window]
Xinyi Ma
Affiliation:
Institute of Entomology, Guizhou University, Guiyang, P. R. China Scientific Observing and Experimental Station of Crop Pest in Guiyang, Ministry of Agriculture and Rural Affairs of the P. R. China, Guiyang, P. R. China
Zhiyong Yin
Affiliation:
Institute of Entomology, Guizhou University, Guiyang, P. R. China Scientific Observing and Experimental Station of Crop Pest in Guiyang, Ministry of Agriculture and Rural Affairs of the P. R. China, Guiyang, P. R. China
Haiyin Li
Affiliation:
Institute of Entomology, Guizhou University, Guiyang, P. R. China Scientific Observing and Experimental Station of Crop Pest in Guiyang, Ministry of Agriculture and Rural Affairs of the P. R. China, Guiyang, P. R. China
Jianjun Guo*
Affiliation:
Institute of Entomology, Guizhou University, Guiyang, P. R. China Scientific Observing and Experimental Station of Crop Pest in Guiyang, Ministry of Agriculture and Rural Affairs of the P. R. China, Guiyang, P. R. China
*
Corresponding author: Jianjun Guo; Email: jjguo@gzu.edu.cn
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Abstract

Aspongopus chinensis Dallas 1851, an insect of important economic value, faces challenges in artificial breeding due to mandatory diapause and limited access to wild resources. Heat shock proteins (Hsps) are thought to influence diapause in insects, but little is known about their role in A. chinensis during diapause. This study used genomic methods to identify 25 Hsp genes in A. chinensis, including two Hsp90, 14 Hsp70, four Hsp60 and five small Hsp genes, were located on seven chromosomes, respectively. The gene structures among the same families are relatively conserved. Meanwhile, the motif compositions and secondary structures of A. chinensis Hsps (AcHsps) were predicted. RNA-seq data and fluorescence quantitative PCR analysis showed that there were differences in the expression patterns of AcHsps in diapause and non-diapause stages, and AcHsp70-5 was significantly differentially expressed in both analysis, which was enriched in the pathway of response to hormone. All the results showed that Hsps play an important role in the diapause mechanism of A. chinensis. Our observations highlight the molecular evolution of the Hsp gene and their effect on diapause in A. chinensis.

Keywords

diapauseheat shock proteinresource insect
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 114 , Issue 2 , April 2024 , pp. 260 - 270
Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press

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