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Salt-responsive miRNA-SSR markers from Dongxiang wild rice: characterization and potential application in exploring salt-tolerant genetic resources

Published online by Cambridge University Press:  24 September 2024

Rifang Gao ,
Yong Chen ,
Jie Huang ,
Yingying Mao ,
Yi Zhou Open the ORCID record for Yi Zhou [Opens in a new window] ,
Jiankun Xie  and
Fantao Zhang Open the ORCID record for Fantao Zhang [Opens in a new window]
Rifang Gao
Affiliation:
College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China
Yong Chen
Affiliation:
College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China
Jie Huang
Affiliation:
College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China
Yingying Mao
Affiliation:
College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China
Yi Zhou
Affiliation:
College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China
Jiankun Xie
Affiliation:
College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China
Fantao Zhang*
Affiliation:
College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China
*
Corresponding author: Fantao Zhang; Email: zhang84004@163.com
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Abstract

MicroRNAs (miRNAs) play important roles in regulating salt tolerance in Dongxiang wild rice (DXWR, Oryza rufipogon Griff.). The development of salt-responsive miRNA-simple sequence repeat (SSR) markers will significantly bolster research on DXWR, providing novel tools for exploring salt-tolerant genetic resources and advancing the development of salt-tolerant rice varieties. In the present study, a total of 137 miRNA-SSR markers were successfully developed, specifically derived from miRNAs responsive to salt stress in DXWR. Subsequently, a subset of 20 markers was randomly selected for validation across three distinct DXWR populations, along with 35 modern rice varieties. Notably, 13 of these markers exhibited remarkable polymorphism. The polymorphic markers collectively amplified 52 SSR loci, averaging four alleles per locus. The polymorphism information content values associated with these loci spanned from 0.23 to 0.70, with a mean value of 0.49. Particularly noteworthy is the miR162a-SSR marker, which demonstrated distinct allelic patterns and holds potential as a diagnostic marker for discriminating the salt-tolerant rice varieties from the non-tolerant varieties. This study provides a valuable tool for genetic analysis and precision breeding, facilitating the identification and utilization of valuable salt-tolerant genetic resources.

Keywords

microRNAmolecular breedingsalt toleranceSSR markerwild rice
Type
Short Communication
Information
Plant Genetic Resources , Volume 22 , Issue 5 , October 2024 , pp. 328 - 331
Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press on behalf of National Institute of Agricultural Botany

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