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Establishment of trophoblast cell line derived from buffalo (Bubalus bubalis) parthenogenetic embryo

Published online by Cambridge University Press:  14 October 2024

Sushil K. Mohapatra ,
Anjit Sandhu Open the ORCID record for Anjit Sandhu [Opens in a new window] ,
Prabhat Palta ,
Manoj K. Singh ,
Suresh K. Singla  and
Manmohan S. Chauhan
Sushil K. Mohapatra*
Affiliation:
Department of Animal Biotechnology, College of Veterinary Science and AH, Kamdhenu University, Sardarkrushinagar, Gujarat, India
Anjit Sandhu
Affiliation:
Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India
Prabhat Palta
Affiliation:
Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India
Manoj K. Singh
Affiliation:
Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India
Suresh K. Singla
Affiliation:
Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India
Manmohan S. Chauhan
Affiliation:
Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India
*
Corresponding author: Sushil K. Mohapatra; Email: drsushilmohapatra@gmail.com
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Summary

We have established trophoblast cell lines, from parthenogenesis-derived buffalo blastocysts. The buffalo trophoblast cells were cultured continuously over 200 days and 21 passages. These cells were observed by phase-contrast microscopy for their morphology and characterized by reverse transcriptase polymerase chain reaction and immunofluorescence against trophoblast-specific markers and cytoskeletal proteins. Trophoblast cells showed positive staining for CDX2, a marker of these cells at both blastocyst and cell line levels. Epithelial morphology of these cells was revealed by positive staining against cytokeratins and tubulin but not against vimentin and dolichos biflorus agglutinin. Gene expression profiles of many important placenta-specific genes were studied in the primary trophectoderm outgrowths, which were collected on days 0, 5, 9, 12 and 15 of culture and trophoblast cell line at passages 12–15. Therefore, the trophoblast cell line derived can potentially be used for in vitro studies on buffalo embryonic development.

Keywords

blastocystbuffaloembryoparthenogenetictrophoblast
Type
Research Article
Information
Zygote , Volume 32 , Issue 4 , August 2024 , pp. 328 - 334
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Copyright
© The Author(s), 2024. Published by Cambridge University Press

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