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Molecular mechanisms of cytochrome P450-mediated detoxification of tetraniliprole, spinetoram, and emamectin benzoate in the fall armyworm, Spodoptera frugiperda (J.E. Smith)

Published online by Cambridge University Press:  02 April 2024

Aiyu Wang ,
Yun Zhang ,
Shaofang Liu ,
Chao Xue ,
Yongxin Zhao ,
Ming Zhao ,
Yuanxue Yang  and
Jianhua Zhang Open the ORCID record for Jianhua Zhang [Opens in a new window]
Aiyu Wang
Affiliation:
Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan, China Yellow River Delta Modern Agriculture Research Institute, Shandong Academy of Agricultural Sciences, Dongying, China
Yun Zhang
Affiliation:
Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan, China Yellow River Delta Modern Agriculture Research Institute, Shandong Academy of Agricultural Sciences, Dongying, China
Shaofang Liu
Affiliation:
Key Lab of Bioprocess Engineering of Jiangxi Province, College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang, China
Chao Xue
Affiliation:
Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan, China
Yongxin Zhao
Affiliation:
Shandong Province Yuncheng County Agricultural and Rural Bureau, Yuncheng, China
Ming Zhao
Affiliation:
Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan, China Yellow River Delta Modern Agriculture Research Institute, Shandong Academy of Agricultural Sciences, Dongying, China
Yuanxue Yang
Affiliation:
Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan, China Yellow River Delta Modern Agriculture Research Institute, Shandong Academy of Agricultural Sciences, Dongying, China
Jianhua Zhang*
Affiliation:
Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan, China Yellow River Delta Modern Agriculture Research Institute, Shandong Academy of Agricultural Sciences, Dongying, China
*
Corresponding author: Jianhua Zhang; Email: zhangjianhua198904@163.com
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Abstract

The fall armyworm (FAW) Spodoptera frugiperda (J.E. Smith) is a highly damaging invasive omnivorous pest that has developed varying degrees of resistance to commonly used insecticides. To investigate the molecular mechanisms of tolerance to tetraniliprole, spinetoram, and emamectin benzoate, the enzyme activity, synergistic effect, and RNA interference were implemented in S. frugiperda. The functions of cytochrome P450 monooxygenase (P450) in the tolerance to tetraniliprole, spinetoram, and emamectin benzoate in S. frugiperda was determined by analysing changes in detoxification metabolic enzyme activity and the effects of enzyme inhibitors on susceptibility to the three insecticides. 102 P450 genes were screened via transcriptome and genome, of which 67 P450 genes were differentially expressed in response to tetraniliprole, spinetoram, and emamectin benzoate and validated by quantitative real-time PCR. The expression patterns of CYP9A75, CYP340AA4, CYP340AX8v2, CYP340L16, CYP341B15v2, and CYP341B17v2 were analysed in different tissues and at different developmental stages in S. frugiperda. Silencing CYP340L16 significantly increased the susceptibility of S. frugiperda to tetraniliprole, spinetoram, and emamectin benzoate. Furthermore, knockdown of CYP340AX8v2, CYP9A75, and CYP341B17v2 significantly increased the sensitivity of S. frugiperda to tetraniliprole. Knockdown of CYP340AX8v2 and CYP340AA4 significantly increased mortality of S. frugiperda to spinetoram. Knockdown of CYP9A75 and CYP341B15v2 significantly increased the susceptibility of S. frugiperda to emamectin benzoate. These results may help to elucidate the mechanisms of tolerance to tetraniliprole, spinetoram and emamectin benzoate in S. frugiperda.

Keywords

enzyme activityinsecticideP450 genesRNA interferenceSpodoptera frugiperdasynergistic effect
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 114 , Issue 2 , April 2024 , pp. 159 - 171
Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press

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