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Development of nuclear microsatellite markers in Stizophyllum (Bignoniaceae) using next-generation sequencing

Published online by Cambridge University Press:  05 April 2019

Maila Beyer Open the ORCID record for Maila Beyer [Opens in a new window] ,
Alison Gonçalvez Nazareno Open the ORCID record for Alison Gonçalvez Nazareno [Opens in a new window]  and
Lúcia G. Lohmann Open the ORCID record for Lúcia G. Lohmann [Opens in a new window]
Maila Beyer*
Affiliation:
Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo (USP), Rua do Matão 277, 05508-090, SP, São Paulo, Brazil
Alison Gonçalvez Nazareno
Affiliation:
Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo (USP), Rua do Matão 277, 05508-090, SP, São Paulo, Brazil
Lúcia G. Lohmann*
Affiliation:
Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo (USP), Rua do Matão 277, 05508-090, SP, São Paulo, Brazil
*
*Corresponding authors. E-mail: maila.beyer@gmail.com, llohmann@usp.br
*Corresponding authors. E-mail: maila.beyer@gmail.com, llohmann@usp.br
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Abstract

Here we developed the first set of nuclear microsatellite markers (nSSRs) for Stizophyllum riparium (Bignoniaceae), a widespread species of Neotropical liana. Thirty-two sets of primers were isolated from a genomic dataset obtained with an Illumina HiSeq Platform, and characterized for 57 individuals of S. riparium from three populations. Nine nSSRs were polymorphic and the number of alleles ranged from eight to 29. The unbiased expected heterozygosity per locus ranged from 0.724 to 0.952 and the polymorphic information content values ranged from 0.717 to 0.944. All nine primer pairs also amplified for two closely related species (S. inaequilaterum and S. perforatum). The new set of nuclear markers will be useful for population genetics studies of Stizophyllum as a whole.

Keywords

cross-amplificationgenetic diversityStizophyllum inaequilaterumStizophyllum perforatum
Type
Short Communication
Information
Plant Genetic Resources , Volume 17 , Issue 4 , August 2019 , pp. 382 - 385
Copyright
Copyright © NIAB 2019 

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