The influences of acoustic excitation on the velocity field and mixing characteristic of a jet in cross-flow were investigated in a wind tunnel. The acoustic excitation waves at resonance Strouhal number were generated by a loudspeaker. The time-averaged velocity field and streamlines of the excited elevated transverse jet in the symmetry plane were measured by a high-speed particle image velocimetry. The visual penetration height and spread width were obtained by using an image processing technique. The dispersion characteristics were obtained from the tracer-gas concentration measurement. The results showed that the streamline pattern of the non-excited transverse jet was significantly modified by the acoustic excitation—the bent streamlines evolved from the jet exit escalated and the vortex rings in the jet and tube wakes and the recirculation bubble in the jet wake disappeared. The time-averaged velocity distributions revealed that the excited transverse jet produces large momentum in the up-shooting direction so that the velocity trajectories were located at levels higher than those of the non-excited one. The mixing characteristics, which include the visual penetration height, spread width, and dispersion, were drastically improved by the acoustic excitation due to the changes in the flow structures. The excited transverse jet characterized at larger jet-to-crossflow momentum flux ratios presented larger improvement in the mixing characteristics than at lower jet-to-crossflow momentum flux ratios.