Downy brome is a cleistogamous facultative winter-annual grass weed that invades cropland, pastureland, and ruderal areas in western North America. Glyphosate-resistant downy brome, the first known glyphosate-resistant grass weed in Canada, was confirmed in a glyphosate-resistant canola field in southern Alberta in 2021. A controlled-environment study was conducted to determine the impact of preemergence soil-applied residual herbicides on glyphosate-resistant and susceptible downy brome in two field soils. Flumioxazin/pyroxasulfone (70/89 g ai ha−1), carfentrazone/pyroxasulfone (18/150 g ai ha−1), sulfentrazone/pyroxasulfone (100/100 or 150/150 g ai ha−1), and saflufenacil/pyroxasulfone (36/120 g ai ha−1) resulted in excellent (≥90%) visible control and downy brome biomass reduction 8 wk after treatment (WAT). The low rate of carfentrazone/pyroxasulfone (12/100 g ai ha−1) resulted in good (≥80%) visible control and biomass reduction 8 WAT, while the low and medium rates of saflufenacil/pyroxasulfone (18/60 or 25/84 g ai ha−1) resulted in ≥80% biomass reduction but suppression only (66% to 75%) based on visible control. Flumioxazin alone (105 g ai ha−1) resulted in good visible control (81%) 8 WAT in a sandy loam soil, but poor (13%) control in a clay loam soil. Soil type affected plant growth as evidenced by reduced growth in the untreated sandy loam soil compared to clay loam soil. The glyphosate-resistant population emerged and grew more vigorously than the glyphosate-susceptible population resulting in greater plant densities in the untreated control and some less-effective herbicide treatments. These results suggest that mixtures of a protoporphyrinogen oxidase-inhibiting herbicide with the very-long-chain fatty acid elongase inhibitor pyroxasulfone applied preemergence at ≥89 g ai ha−1 could be effective components of an herbicide layering strategy targeting glyphosate-resistant and glyphosate-susceptible downy brome.

Increased frequency and occurrence of herbicide-resistant biotypes heightens the need for alternative wild oat management strategies. This study aimed to exploit the height differential between wild oat and crops by targeting wild oat between panicle emergence and seed shed timing. Two field studies were conducted either in Lacombe, AB, or Lacombe, AB and Saskatoon, SK, from 2015 to 2017. In the first study, we compared panicle removal methods: hand clipping, use of a hedge trimmer, and a selective herbicide crop topping application to a weedy check and an industry standard in-crop herbicide application in wheat. These treatments were tested early (at panicle emergence), late (at initiation of seed shed), or in combination at one location over 3 yr. In the second study, we investigated optimal timing of panicle removal via a hedge trimmer with weekly removals in comparison to a weedy check in wheat and lentil. This study was conducted at two locations, Lacombe, AB, and Saskatoon, SK, over 3 yr. Among all the tested methods, the early crop topping treatment consistently had the largest impact on wild oat density, dockage, seedbank, and subsequent year crop yield. The early (at panicle emergence) or combination of early and late (at initiation of seed shed) treatments tended to reduce wild oat populations the following season the most compared to the late treatments. Subsequent wild oat populations were not influenced by panicle removal timing, but only by crop and location interactions. Panicle removal timing did significantly affect wild oat dockage in the year of treatment, but no consistent optimal timing could be identified. However, the two studies together highlight additional questions to be investigated, as well as the opportunity to manage wild oat seedbank inputs at the panicle emergence stage of the wild oat lifecycle.

Glyphosate-resistant (GR) canola is a widely grown crop across western Canada and has quickly become a prolific volunteer weed. Glyphosate-resistant soybean is rapidly gaining acreage in western Canada. Thus, there is a need to evaluate herbicide options to manage volunteer GR canola in GR soybean crops. We conducted an experiment to evaluate the efficacy of various PRE and POST herbicides applied sequentially to volunteer GR canola and to evaluate soybean injury caused by these herbicides. Trials were conducted across Saskatchewan and Manitoba in 2014 and 2015. All treatments provided a range of suppression (>70%) to control (>80%) of volunteer canola. All treatments with the exception of the glyphosate-treated control reduced aboveground canola biomass by an average of 96%. As well, canola seed contamination was reduced from 36% to less than 5% when a PRE and POST herbicide were both used. Moreover, all combinations of herbicides used had excellent crop safety (<10%). All PRE and POST herbicide combinations provided better control of volunteer canola compared with the glyphosate-only control, but tribenuron followed by bentazon and tribenuron followed by imazamox plus bentazon provided solutions that were low cost, currently available (registered in western Canada), and had the potential to minimize development of herbicide resistance in other weeds.

The pyridine carboxylic acid (PCA) herbicide family can exhibit differential activity within and among plant species, despite molecular resemblances. Aminocyclopyrachlor (AMCP), a pyrimidine carboxylic acid, is a recently discovered compound with similar use patterns to those of the PCA family; however, relative activity among PCAs and AMCP is not well understood. Therefore, the objective of this study was to quantify relative activity among aminopyralid, picloram, clopyralid, triclopyr, and AMCP in canola, squash, and okra using dose-response whole-plant bioassays. Clopyralid was less active than all other herbicides in all species and did not fit dose-response models. Aminopyralid and picloram performed similarly in squash (ED50 = 21.1 and 23.3 g ae ha−1, respectively). Aminopyralid was 3.8 times and 1.7 times more active than picloram in canola (ED50 = 60.3 and 227.7 g ha−1, respectively) and okra (ED50 = 10.3 and 17.3 g ha−1, respectively). Triclopyr (ED50 = 37.3 g ha−1) was more active than AMCP (ED50 = 112.9 g ha−1) and picloram in canola. Aminocyclopyrachlor (ED50 = 6.6 g ha−1) and triclopyr (ED50 = 7.8 g ha−1) were more active in squash than aminopyralid and picloram. In okra, AMCP (ED50 = 14.6 g ha−1) and aminopyralid (ED50 = 10.3 g ha−1) performed similarly but were more active than triclopyr (ED50 = 88.2 g ha−1). Herbicidal activity among AMCP and PCAs was vastly different despite molecular similarities that could be due to variable target-site sensitivity among species.

In recent years, soybean acreage has increased significantly in western Canada. One of the challenges associated with growing soybean in western Canada is the control of volunteer glyphosate-resistant (GR) canola, because most soybean cultivars are also glyphosate resistant. The objective of this research was to determine the impact of soybean seeding rate and planting date on competition with volunteer canola. We also attempted to determine how high seeding rate could be raised while still being economically feasible for producers. Soybean was seeded at five different seeding rates (targeted 10, 20, 40, 80, and 160 plants m−2) and three planting dates (targeted mid-May, late May, and early June) at four sites across western Canada in 2014 and 2015. Soybean yield consistently increased with higher seeding rates, whereas volunteer canola biomass decreased. Planting date generally produced variable results across site-years. An economic analysis determined that the optimal rate was 40 to 60 plants m−2, depending on market price, and the optimal planting date range was from May 20 to June 1.

In Australia, widespread evolution of multi-resistant weed populations has driven the development and adoption of harvest weed seed control (HWSC). However, due to incompatibility of commonly used HWSC systems with highly productive conservation cropping systems, better HWSC systems are in demand. This study aimed to evaluate the efficacy of the integrated Harrington Seed Destructor (iHSD) mill on the seeds of Australia’s major crop weeds during wheat chaff processing. Also examined were the impacts of chaff type and moisture content on weed seed destruction efficacy. Initially, the iHSD mill speed of 3,000 rpm was identified as the most effective at destroying rigid ryegrass seeds present in wheat chaff. Subsequent testing determined that the iHSD mill was highly effective (>95% seed kill) on all Australian crop weeds examined. Rigid ryegrass seed kill was found to be highest for lupin chaff and lowest in barley, with wheat and canola chaff intermediate. Similarly, wheat chaff moisture reduced rigid ryegrass seed kill when moisture level exceeded 12%. The broad potential of the iHSD mill was evident, in that the reductions in efficacy due to wide-ranging differences in chaff type and moisture content were relatively small (≤10%). The results from these studies confirm the high efficacy and widespread suitability of the iHSD for use in Australian crop production systems. Additionally, as this system allows the conservation of all harvest residues, it is the best HWSC technique for conservation cropping systems.

Interest in planting mixtures of cover crop species has grown in recent years as farmers seek to increase the breadth of ecosystem services cover crops provide. As part of a multidisciplinary project, we quantified the degree to which monocultures and mixtures of cover crops suppress weeds during the fall-to-spring cover crop growing period. Weed-suppressive cover crop stands can limit weed seed rain from summer- and winter-annual species, reducing weed population growth and ultimately weed pressure in future cash crop stands. We established monocultures and mixtures of two legumes (medium red clover and Austrian winter pea), two grasses (cereal rye and oats), and two brassicas (forage radish and canola) in a long fall growing window following winter wheat harvest and in a shorter window following silage corn harvest. In fall of the long window, grass cover crops and mixtures were the most weed suppressive, whereas legume cover crops were the least weed suppressive. All mixtures also effectively suppressed weeds. This was likely primarily due to the presence of fast-growing grass species, which were effective even when they were seeded at only 20% of their monoculture rate. In spring, weed biomass was low in all treatments due to winter kill of summer-annual weeds and low germination of winter annuals. In the short window following silage corn, biomass accumulation by cover crops and weeds in the fall was more than an order of magnitude lower than in the longer window. However, there was substantial weed seed production in the spring in all treatments not containing cereal rye (monoculture or mixture). Our results suggest that cover crop mixtures require only low seeding rates of aggressive grass species to provide weed suppression. This creates an opportunity for other species to deliver additional ecosystem services, though careful species selection may be required to maintain mixture diversity and avoid dominance of winter-hardy cover crop grasses in the spring.

Shortawn foxtail is an invasive grass weed infesting winter wheat and canola production in China. A better understanding of the germination ecology of shortawn foxtail would help to develop better control strategies for this weed. Experiments were conducted under laboratory conditions to evaluate the effects of various abiotic factors, including temperature, light, pH, osmotic stress, salt concentration, and planting depth, on seed germination and seedling emergence of shortawn foxtail. The results showed that the seed germination rate was greater than 90% over a wide range of constant (5 to 25C) and alternating (15/5 to 35/25C) temperatures. Maximum germination occurred at 20C or 25/15C, and no germination occurred at 35C. Light did not appear to have any effect on seed germination. Shortawn foxtail germination was 27% to 99% over a pH range of 4 to 10, and higher germination was obtained at alkaline pH values ranging from 7 to 10. Seed germination was sensitive to osmotic potential and completely inhibited at an osmotic potential of −0.6 MPa, but it was tolerant to salinity: germination even occurred at 200 mM NaCl (5%). Seedling emergence was highest (98%) when seeds were placed on the soil surface but declined with the increasing burial depth. No seedlings emerged when seeds were buried 6-cm deep. Deep tillage could be an effective measure to limit seed germination from increased burial depth. The results of this study will lead to a better understanding of the requirements for shortawn foxtail germination and emergence and will provide information that could contribute to its control.

Chlorsulfuron was applied to wheat at rates of 0, 10, 20, and 40 g ai ha-1 in three locations at Lethbridge and Brooks between 1980 and 1984. The soil pH and organic matter contents at Lethbridge and Brooks were 8.0 and 2.1% and 7.4 and 3.0%, respectively. Rotational crops were seeded for up to 7 yr after chlorsulfuron application. The criteria used to indicate when susceptible crops will grow safely were no significant yield loss at the 40 g ha-1 rate and the magnitude of the upper confidence limit for this difference. The following times after application before crop yields were not affected were required at Lethbridge: barley, 2 yr; canola, 3 yr; peas, beans, 4 yr; flax and potatoes, 5 yr; alfalfa and sugarbeets, 6 yr; and lentils at least 7 yr. The effect of chlorsulfuron residues in the soil on rotational crops appeared to be similar at Brooks and at Lethbridge. Estimated chlorsulfuron concentrations were related to the tolerance of rotational crops.

Field experiments were conducted for 3 yr to determine the effect of various biological and physical factors on the operation of the weed-sensing Detectspray system. Plant detection is achieved by sensors measuring differential reflectance of red and near-infrared wavelengths of light from green plants, crop residues, and soil. Weed detection was greatly reduced 70 to 80 min after sunrise and before sunset when operated at lat 50°N because of reduced solar irradiance. Tall, dense-standing crop stubble limited detection of small weeds at the soil surface. Weed detection varied with plant species. Canola with three to four leaves consistently was detected, but wheat or green foxtail usually required five to six leaves to be detected. Small weeds were detected if present at densities greater than 70 plants m−2. Growers and commercial applicators need to be aware of the limitations of the Detectspray system to use it effectively to control weeds with concurrent reductions in herbicide use.

To determine if cell damage could account for chlorsulfuron inhibition of assimilate transport, the ultrastructure of companion cells and surrounding mesophyll cells was examined following chlorsulfuron treatment. Six hours after treatment of canola leaves, thylakoids in companion cells were swollen and chloroplast structures in mesophyll cells were disorganized. Three days after treatment, the treated leaves contained more starch granules in companion cells than corresponding control leaves, presumably as a result of reduced assimilate export. We conclude that the inhibition of assimilate export in chlorsulfuron-treated canola leaves is associated, at least in part, with the ultrastructural changes described, particularly those in companion cells.

Postemergence control of broadleaf weeds in canola with DPX A7881 was studied under controlled-environment and field conditions. DPX A7881 controlled weeds of the Cruciferae family such as wild mustard, field pennycress, and flixweed, which are not controlled with existing herbicides used for weed control in canola. Weed control was better when DPX A7881 was applied at the two-leaf than at the six-leaf stage of the weeds. At the two-leaf stage, control of flixweed was achieved with 15 g ai/ha of DPX A7881; 20 to 30 g ai/ha was required for control of wild mustard and field pennycress. Control of redroot pigweed required 30 g ai/ha. Common lambsquarters was not controlled in this study with DPX A7881 up to 60 g ai/ha. Control of weeds with DPX A7881 increased canola yields in this study without altering the important quality components: oil content, 1000-kernel weight, and green seed content. DPX A7881 provides a postemergence alternative to existing herbicides for broadleaf weed control in canola.

Our objective was to determine if the chlorsulfuron-induced reduction in assimilate export from leaves can be attributed to a shortage of carbohydrates. Treated canola leaves showed no reduction in carbon fixation or carbohydrate production during the first 24 h, but they exuded only 17 to 27% of the amount of sucrose exuded by corresponding control leaves. Exposure of the leaves to higher concentrations of CO2 (500 vs. 350 μl L−1) resulted in greater net carbon exchange and higher starch content, but failed to overcome the reduction in sucrose export, presumably because of increased carbon allocation to starch.

Five sulfonylurea herbicides were applied to Typic Boroll soil with pH 8.0 and 2% organic matter in the field and growth chamber to assess their residual effects on following crops. Under irrigated conditions, triasulfuron at 22 g/ha reduced the growth of alfalfa, canola, corn, lentil, pea, potato, and sugarbeet the year after application but caused no injury after two years. Alfalfa GR50 (rate required to reduce growth by 50%) values immediately after application of chlorsulfuron, triasulfuron, and metsulfuron were < 2.2 g/ha and similar in the growth chamber and field. Alfalfa and canola GR50 values immediately after thifensulfuron application in the growth chamber were ≥ 90 g/ha. Alfalfa, canola, flax, and sugarbeet GR50 for tribenuron in the growth chamber were < 4 g/ha. Crop response curves developed by growth chamber experiments could replace many of the multiple site and crop field experiments now required to develop recropping guidelines for residual herbicides.

Imazamox-resistant hybrids resulted from a cross between jointed goatgrass and an imazamox-resistant wheat (cv. FS-4 IR wheat). Two imazamox-resistant hybrids were discovered in a research plot where FS-4 IR wheat seed had been replanted from the harvest of an imazamox efficacy study conducted the year before at a different location. These hybrid plants survived imazamox applied at 0.053 and 0.069 kg ai ha−1 in the field and produced seven viable seeds (BC1). This seed germinated, and chromosomes were counted from the roots (2N number ranged from 39 to 54). In the greenhouse, six of the seven plants survived an application of 0.072 kg ai ha−1imazamox, which confirmed that the resistance trait had been passed to these progeny. A large amount of phenotypic variation was observed in the mature BC1 plants. A genetic description of the movement of the resistant gene is proposed based on the case of the gene being located on the D and the A or B genomes. Management strategies to reduce the occurrence of herbicide-resistant hybrids are presented.

The effect of imazethapyr and imazamethabenz on following crops was tested in southern Alberta, on Dark Brown and Brown Chernozemic soils, to assess the potential restrictions placed on cropping sequences by the use of these herbicides. Imazamethabenz reduced the yield of sugarbeet seeded one year after application. After imazethapyr application there is risk of yield loss with flax, corn, meadow bromegrass, mustard, sunflower, timothy, and wheat seeded one year later; canola seeded up to two years later; and sugarbeet and potato seeded up to three years later. Legume crops and intermediate wheatgrass may be seeded the year after application with little risk of yield loss. The required recropping intervals limit the use of imazethapyr for weed control in pea, alfalfa, or dry bean in cropping sequences that include sugarbeet, canola, or potato.

Unintentional herbicide resistance gene stacking in canola may alter the sensitivity of volunteers to herbicides of alternative modes of action commonly used for their control. Greenhouse experiments were conducted to investigate the response of three single-herbicide–resistant (HR) cultivars (glyphosate, glufosinate, imidazolinone), one non-HR cultivar, and seven multiple (double or triple)–HR experimental lines to 2,4-D (amine and ester), MCPA ester, and metribuzin applied at the two- to three-leaf stage and of one non-HR and four HR cultivars (glyphosate, glufosinate, imidazolinone, bromoxynil) to 2,4-D amine applied at two growth stages (two- to three-leaf stage and five- to six-leaf stage). All canola cultivars or lines treated at the two- to three-leaf stage responded similarly to increasing doses of each of the three herbicides. At the five- to six-leaf stage, however, the bromoxynil HR cultivar was less sensitive to 2,4-D than the other cultivars. The results of this study suggest that canola with multiple-herbicide–resistance traits does not differ from cultivars that are non-HR or single HR in its sensitivity to herbicides commonly used to control volunteers. All volunteers, whether non-HR, single HR, or multiple HR, should be treated when plants are most sensitive to herbicides (two- to four-leaf stage) to reduce their interference against crops and their perpetuation of gene flow.

In western Canada, seasonal seedling recruitment has been reported in weedy canola populations, and seed persistence has been linked to the secondary seed dormancy potential of a genotype. Temperature influences secondary seed dormancy induction in this species. In these experiments we (1) investigated the influence of temperature and osmotic potential on secondary seed dormancy induction in canola, (2) related these to seedbank dynamics and seedling recruitment of two canola genotypes with different seed dormancy potentials in the field, and (3) investigated the influence of residue, burial depth, and soil type on seedbank dynamics and seedling recruitment in the field. In the laboratory, rates of seed dormancy induction were positively correlated to increasing temperatures and water stress. The role of temperature was approximately threefold more important to seed dormancy development than was osmotic potential within the tested ranges of these variables. In the field, seasonal seedbank dynamics of canola buried at 10 cm were strongly influenced by a genotype's inherent potential for secondary dormancy. An increase in the ungerminable portion of the seedbank was observed in the high-dormancy genotype as soil temperatures increased during spring. This did not occur in the low-dormancy genotype, resulting in sixfold less seed persistence in this genotype by midsummer, by which time, the total remaining seedbank was ungerminable in both genotypes. At the 1-cm burial depth, most of the seedbank was depleted by midsummer of the year after seedbank establishment because of high seedbank mortality in all treatments. Thus, the seasonal recruitment behavior in canola was primarily a function of seed death in the shallow seedbank and a shift to an ungerminable state in the deep seedbank.

Greenhouse experiments were conducted to determine the effect of glyphosate on efficacy of bentazon for glyphosate-resistant (GR) canola control and of quizalofop for GR corn control. Control also was evaluated for glyphosate plus bentazon on wild buckwheat and wheat and glyphosate plus quizalofop on velvetleaf. Glyphosate plus bentazon synergistically controlled GR canola and wild buckwheat but were antagonistic for wheat control. Glyphosate plus quizalofop were additive for control of GR corn and velvetleaf. Inert ingredients in glyphosate formulations, i.e., cationic surfactant, NH4, or K, contributed to glyphosate synergism of bentazon, but the major contribution came from glyphosate itself. Efficacy of glyphosate plus bentazon on GR canola was enhanced by ammonium nitrate (AMN), ammonium sulfate (AMS), nonionic surfactant (NIS), or silicone surfactant (SiS) but was slightly decreased by methylated seed oil (MSO) or petroleum oil concentrate. AMN, AMS, NIS, and SiS partially overcame the antagonism of bentazon to glyphosate for wheat control. NIS enhanced phytotoxicity of glyphosate plus quizalofop to GR corn and velvetleaf, but the enhancement was less than by SiS or MSO to GR corn and SiS or AMS to velvetleaf.

A 2-yr (2009 to 2010), no-till (direct-seeded) “follow-up” study was conducted at five western Canada sites to determine weed interference impacts and barley and canola yield recovery after 4 yr of variable crop inputs (seed, fertilizer, herbicide). During the initial period of the study (2005 to 2008), applying fertilizer in the absence of herbicides was often worse than applying no optimal inputs; in the former case, weed biomass levels were at the highest levels (2,788 to 4,294 kg ha−1), possibly due to better utilization of nutrients by the weeds than by the crops. After optimal inputs were restored (standard treatment), most barley and canola plots recovered to optimal yield levels after 1 yr. However, 4 yr with all optimal inputs but herbicides led to only 77% yield recovery for both crops. At most sites, when all inputs were restored for 2 yr, all plots yielded similarly to the standard treatment combination. Yield “recovery” occurred despite high weed biomass levels (> 4,000 kg ha−1) prior to the first recovery year and despite high wild oat seedbank levels (> 7,000 seeds m−2) at the end of the second recovery year. In relatively competitive narrow-row crops such as barley and canola, the negative effects of high soil weed seedbanks can be mitigated if growers facilitate healthy crop canopies with appropriate seed and fertilizer rates in combination with judicious herbicide applications to adequately manage recruited weeds.