PRE herbicides have been reported to injure both the foliage and roots of hybrid bermudagrass turf established in sand culture. Research was conducted to evaluate the influence of reed-sedge peat moss (RSPM) on hybrid bermudagrass injury following PRE herbicide applications to plants established in sand culture. Washed sod plugs were established in mini-rhizotrons constructed with sand root-zones varying in organic carbon content (0.000, 0.003, 0.007, and 0.012 kg kg−1). Herbicide treatments included indaziflam (35 and 52.5 g ai ha−1) and prodiamine (840 g ai ha−1). Significant foliar injury was only observed with indaziflam at 52.5 g ha−1. When applied to plants in sand with no detectable (0.000 kg kg−1) organic carbon, foliar injury measured 61% by 6 wk after treatment. Comparatively, injury with indaziflam at 52.5 g ha−1 was reduced by 40% with applications to plants established in sand with 0.007 kg kg−1 organic carbon. Root length, root length density, and root surface area were greatest in sand-based root zones with ≥ 0.007 kg kg−1 organic carbon regardless of herbicide treatment; however, only indaziflam (52.5 g ha−1) and prodiamine-treated plants exhibited diminished root parameters relative to the nontreated check. Data in the current study illustrate that RSPM can affect above- and belowground injury following PRE herbicide applications to hybrid bermudagrass in sand root-zones.