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Describes a range of physical techniques that can be applied to bacterial biophysics including sample culture, flow cytometry, microscopy, photonics, NMR, mass spectrometry and electrophoresis.
We present the Okinawa Institute of Science and Technology – Taylor–Couette set-up (OIST-TC), a new experimental set-up for investigating turbulent Taylor–Couette (TC) flow. The set-up has independently rotating inner and outer cylinders, and can achieve Reynolds numbers up to $10^6$. Noteworthy aspects of its design include innovative strategies for temperature control and vibration isolation. As part of its flow-measurement instrumentation, we have implemented the first ‘flying hot-wire’ configuration to measure the flow velocity whilst either or both cylinders are rotating. A significant challenge for obtaining reliable measurements from sensors within the inner cylinder is the data distortion resulting from electrical and electromagnetic interference along the signal pathway. Our solution involves internal digitization of sensor data, which provides notable robustness against noise sources. Additionally, we discuss our strategies for efficient operation, outlining custom automation tools that streamline both data processing and operational control. We hope this documentation of the salient features of OIST-TC is useful to researchers engaged in similar experimental studies that delve into the enchanting world of turbulent TC flow.
Traditional pulsar surveys have primarily employed time-domain periodicity searches. However, these methods are susceptible to effects like scattering, eclipses, and orbital motion. At lower radio frequencies ($\lesssim$300 MHz), factors such as dispersion measure and pulse broadening become more prominent, reducing the detection sensitivity. On the other hand, image domain searches for pulsars are not limited by these effects and can extend the parameter space to regions inaccessible to traditional search techniques. Therefore, we have developed a pipeline to form 1-second full Stokes images from offline correlated high time-resolution data from the Murchison Widefield Array (MWA). This led to the development of image-based methodologies to identify new pulsar candidates. In this paper, we applied these methodologies to perform a low-frequency image-based pulsar census of the galactic plane (12 MWA observations, covering $\sim$6 000 $\textrm{deg}^\textrm{2}$ sky). This work focuses on the detection of the known pulsar population which were present in the observed region of the sky using both image-based and beamformed methods. This resulted in the detection of 83 known pulsars, with 16 pulsars found only in Stokes I images but not in periodicity searches applied in beamformed data. Notably, for 14 pulsars these are the first reported low-frequency detections. This underscores the importance of image-based searches for pulsars that may be undetectable in time-series data, due to scattering and/or dispersive smearing at low frequencies. This highlights the importance of low-frequency flux density measurements in refining pulsar spectral models and investigating the spectral turnover of pulsars at low frequencies.
This chapter demystifies orchestration by offering insights into how a good understanding of balance, timbre, and instrumental technique is used to imagine and create interesting sonorities. The chapter begins with an overview of the development of the modern orchestra, before explaining how composers in the twentieth and twenty-first centuries have explored the limits of idiomatic instrumental writing to create dramatic and compelling orchestral textures. It concludes with an explanation of how to approach orchestrating from a ‘short’ piano score.
Research study complexity refers to variables that contribute to the difficulty of a clinical trial or study. This includes variables such as intervention type, design, sample, and data management. High complexity often requires more resources, advanced planning, and specialized expertise to execute studies effectively. However, there are limited instruments that scale study complexity across research designs. The purpose of this study was to develop and establish initial psychometric properties of an instrument that scales research study complexity.
Methods:
Technical and grammatical principles were followed to produce clear, concise items using language familiar to researchers. Items underwent face, content, and cognitive validity testing through quantitative surveys and qualitative interviews. Content validity indices were calculated, and iterative scale revision was performed. The instrument underwent pilot testing using 2 exemplar protocols, asking participants (n = 31) to score 25 items (e.g., study arms, data collection procedures).
Results:
The instrument (Research Complexity Index) demonstrated face, content, and cognitive validity. Item mean and standard deviation ranged from 1.0 to 2.75 (Protocol 1) and 1.31 to 2.86 (Protocol 2). Corrected item-total correlations ranged from .030 to .618. Eight elements appear to be under correlated to other elements. Cronbach’s alpha was 0.586 (Protocol 1) and 0.764 (Protocol 2). Inter-rater reliability was fair (kappa = 0.338).
Conclusion:
Initial pilot testing demonstrates face, content, and cognitive validity, moderate internal consistency reliability and fair inter-rater reliability. Further refinement of the instrument may increase reliability thus providing a comprehensive method to assess study complexity and related resource quantification (e.g., staffing requirements).
Whilst Richard Wagner has long been acknowledged as one of the central figures in the history of orchestration, his treatment of the orchestra has only rarely received scholarly attention. This chapter uses a series of analytical vignettes to examine Wagner’s approach to the orchestra, each addressing a paradox or opposition. The aim is not to expound some grand, overarching narrative, but, instead, to use the friction between competing factors to demonstrate the inherent complexity of Wagner’s approach to the orchestra. The multidimensionality of Wagner’s orchestration is also seen in the highly nuanced interaction of its three main parameters: texture, timbre, and spatiality. The development of Wagner’s orchestration over his lifetime is not presented as a continuous progression; the individuality of each of Wagner’s scores – and even of scenes within those operas – reflects the inseparability of Wagner’s orchestration from its dramatic motivation.
Radiocarbon (14C) dating of sediment deposition around Antarctica is often challenging due to heterogeneity in sources and ages of organic carbon in the sediment. Chemical and thermochemical techniques have been used to separate organic carbon when microfossils are not present. These techniques generally improve on bulk sediment dates, but they necessitate assumptions about the age spectra of specific molecules or compound classes and about the chemical heterogeneity of thermochemical separations. To address this, the Rafter Radiocarbon Laboratory has established parallel ramped pyrolysis oxidation (RPO) and ramped pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS) systems to thermochemically separate distinct carbon fractions, diagnose the chemical composition of each fraction, and target suitable RPO fractions for radiocarbon dating. Three case studies of sediment taken from locations around Antarctica are presented to demonstrate the implementation of combined RPO-AMS and Py-GC-MS to provide more robust age determination in detrital sediment stratigraphy. These three depositional environments are good examples of analytical and interpretive challenges related to oceanographic conditions, carbon sources, and other factors. Using parallel RPO-AMS and Py-GC-MS analyses, we reduce the number of radiocarbon measurements required, minimize run times, provide context for unexpected 14C ages, and better support interpretations of radiocarbon measurements in the context of environmental reconstruction.
As the popularity of K-pop has grown around the globe, the number and scope of K-pop studies have also expanded. While many have provided important insights into socioeconomic aspects of K-pop, the music itself has rarely been at the center of discussion. The purpose of this chapter is to help fill the gap by examining the sound of K-pop, focusing on its musical elements such as melody, rhythm, and instrumentation. This approach involves close listening and reading of select songs covering various stylistic genres and analyzing their sound using the language of music theory. By so doing, this study will identify and offer an understanding of common musical structures used in K-pop songs. Furthermore, the chapter attempts to respond to the question asked most frequently in the author’s K-pop class: How is K-pop different from popular music of the West? To that end, a comparative analysis is conducted between K-pop songs and Western pop music. Among the styles of songs examined are bubblegum popular music, ballads, and songs that quote Korean traditional music, the types of music that are most revealing in addressing the question of distinctiveness of K-pop songs.
Leica’s current IHC instrument is the Bond III. A review is conducted by a medical scientist using it on a daily basis so that an honest evaluation is afforded from first-hand experience. Topics of interest include the machinery Leica employs for epitope retrieval and the coverplate technology to assist in reagent delivery. A discussion about proprietary reagents and consumables whilst highlighting the various components of the machine is provided. There are tips and tricks offered to get the most out of the platform. Programming stain protocols for both chromogenic, fluorescence and double labelling IHC are specified along with equipment servicing and maintenance requirements. The reader in essence, gets to appreciate what it is really like to operate and work with the Bond III. The chapter concludes with both good and bad aspects of this form of automation and some opportunities for improvement.
Immunohistochemistry robotics and automation as defined by Agilent comes in the form of the Dako Omnis. An appraisal is given by a medical scientist with intimate knowledge of the principles behind the technology, the various machine components and Dako’s proprietary reagents. Daily operations with the instrument allow for an honest review of stain protocols, workflow logistics and maintenance obligations. An explanation of the unique dynamic gap technology is provided along with in-built quality assurance measures. As the Omnis is a new instrument when compared to Leica’s Bond III and Ventana’s BenchMark ULTRA, discussions are based upon the good and bad points of both the hardware and the software aspects. The reader should get an idea of how the Omnis produces stained slides and the capabilities of the machine.
Roche’s answer to current immunohistochemistry automation is the Ventana BenchMark ULTRA. An evaluation of the machine’s design, operation and maintenance is provided from a user’s viewpoint. Technologies which differentiate Ventana from other manufacturers are discussed in detail. The liquid coverslip, hapten-based detection system and random access points are examples of these differences when compared to traditional coverplate and polymer technology as used by other vendors. A commentary about proprietary reagents and staining protocols are offered along with advice on achieving workflow efficiencies. Discovering quality control measures of the instrument is another topic covered. Ultimately, it is hoped the reader understands the intricacies of this platform and the advantages and disadvantages of such a system, and gains a sense of the technology behind the BenchMark ULTRA.
The world of environmental microscopy provides the possibility to study and analyze transformations and reactions during realistic conditions to understand the processes better. We report on the design and development of a metal-organic chemical vapor deposition (MOCVD) system integrated with an environmental transmission electron microscope intended for real-time investigations of crystal growth. We demonstrate methods for achieving a wide range of precisely controlled concentrations of precursor gas at the sample, as well as for calibrating the sample partial pressure using the pressure measured elsewhere in the microscope column. The influences of elevated temperature and reactive gas within the pole-piece gap are evaluated with respect to imaging and spectroscopy. We show that X-ray energy-dispersive spectroscopy can be strongly affected by temperatures beyond 500$^{\circ }$C, while the spatial resolution is largely unaffected by heat and microscope pressure for the relevant conditions. Finally, the influence of the electron beam on the investigated processes is discussed. With this work, we aim to provide crucial input in the development of advanced in situ electron microscopy systems for studies of complex reactions in real time under realistic conditions, for instance as used during formation of semiconductor crystals.
Atomic probe tomography (APT) is able to generate three-dimensional chemical maps in atomic resolution. The required instruments for APT have evolved over the last 20 years from an experimental to an established method of materials analysis. Here, we describe the realization of a new modular instrument concept that allows the direct attachment of APT to a dual-beam SEM microscope with the main achievement of fast and direct sample transfer and high flexibility in chamber and component configuration. New operational modes are enabled regarding sample geometry, alignment of tips, and the microelectrode. The instrument is optimized to handle cryo-samples at all stages of preparation and storage. It comes with its own software for evaluation and reconstruction. The performance in terms of mass resolution, aperture angle, and detection efficiency is demonstrated with a few application examples.
In 1978, Bracewell suggested the technique of nulling interferometry to directly image exoplanets which would enable characterisation of their surfaces, atmospheres, weather, and possibly determine their capacity to host life. The contrast needed to discriminate starlight reflected by a terrestrial-type planet from the glare of its host star lies at or beyond a forbidding
$10^{-10}$
for an exo-Earth in the habitable zone around a Sun-like star at near-infrared wavelengths, necessitating instrumentation with extremely precise control of the light. Guided Light Interferometric Nulling Technology (GLINT) is a testbed for new photonic devices conceived to overcome the challenges posed by nulling interferometry. At its heart, GLINT employs a single-mode nulling photonic chip fabricated by direct-write technology to coherently combine starlight from an arbitrarily large telescope at 1 550 nm. It operates in combination with an actuated segmented mirror in a closed-loop control system, to produce and sustain a deep null throughout observations. The GLINT South prototype interfaces the 3.9-m Anglo-Australian Telescope and was tested on a sample of bright Mira variable stars. Successful and continuous starlight injection into the photonic chip was achieved. A statistical model of the data was constructed, enabling a data reduction algorithm to retrieve contrast ratios of about
$10^{-3}$
. As a byproduct of this analysis, stellar angular diameters that were below the telescope diffraction limit (
$\sim$
100 mas) were recovered with 1
$\sigma$
accuracy and shown to be in agreement with literature values despite working in the seeing-limited regime. GLINT South serves as a demonstration of the capability of direct-write photonic technology for achieving coherent, stable nulling of starlight, which will encourage further technological developments towards the goal of directly imaging exoplanets with future large ground based and space telescopes.
This article considers the function of twenty-two hand-colored prints of mathematical instruments in Tycho Brahe's Astronomiae instauratae mechanica (Instruments of the renewed astronomy; 1598), a hand-painted presentation treatise dedicated to Emperor Rudolf II and conferred on a network of individuals connected to the imperial court in Prague. Although the accompanying text communicates the instruments’ use and composition, the images demand close inspection because they articulate Brahe's observationally driven astronomy. They do so through structured, repeated, and consecutive representations; through expanded viewer access, achieved by adhering to multiple perspectives; through the juxtaposition of colors, which focuses attention on the heads of the instruments (the part that does the measuring); and through the use of gold paint, which emphasizes the head and brings to mind the very metallic nature of the instruments. Much like an astronomer taking multiple measurements of cosmological phenomena, these images allow viewers and readers, as they leaf through the pages of the treatise, to become virtual participants in Brahe's instauration of astronomy.
The essential aspects of the modern design and development process for radial flow turbocompressors are described. The different phases of the design process are described, including the conceptual design of the compressor type, the preliminary design of the components, the geometry specification of the ducts and blade rows, the blade-to-blade design, the throughflow design, 3D CFD performance analysis and FEM mechanical analysis. The final decision about the quality of a design is made through compressor testing. The chapter concludes with a section on the testing of compressors; this includes a discussion of the different types of tests, testing methods, standards, guidelines and procedures. Information about suitable instrumentation is also provided. Finally, there is a short review of recent experimental studies from some of the most active research groups with experimental rigs.
Do you remember the first time you heard the music of Igor Stravinsky? Or modernist music? My own teenage introduction to both was Ragtime (1917–18), our music teacher helping us join the dots between its particular strand of twentieth-century classical music and Scott Joplin’s evergreen rag, ‘The Entertainer’ (1902), which the pianists among us would struggle to play.1 Looking back, the muffled giggling which Ragtime provoked was due as much to the jolting introduction of its faint and weird-sounding cimbalom as to the relentless discontinuities that shape its phrasing, melody and timbre. To hear Stravinsky repeatedly is to understand how these innovations relate to one another, but the shock of having to process his music for the first time was real and literally physical. Here were strange folk- and jazz-inspired sounds, far removed from the Classical and Romantic orchestras that had framed our expectations of so-called classical music until that point. Ragtime’s sound was, and remains, quite alien.