Skip to main content Accessibility help

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
×
  1. Home
  2. Search

Refine search

Refine search

Access:
Content type:
Publication date:
Apply   From and To filters
Subject: Show more
Journals Show more
Publishers: Show more
Societies Show more
Series: Show more
Collections: Show more

Actions for selected content:

Select all | Deselect all
  • View selected items
  • Export citations
  • Download PDF (zip)
  • Save to Kindle
  • Save to Dropbox
  • Save to Google Drive

Save Search

You can save your searches here and later view and run them again in "My saved searches".

Please provide a title, maximum of 40 characters.
Cancel
×

2 results

  • Chapter 8 - Artificial Oocyte Activation After ICSI

  • Edited by Gianpiero D. Palermo, Zsolt Peter Nagy
  • Book:
    Manual of Intracytoplasmic Sperm Injection in Human Assisted Reproduction
    Published online:
    02 December 2021
    Print publication:
    25 November 2021, pp 80-86

  • Summary

    It is generally accepted that the presence of a presumably normal spermatozoon does not guarantee oocyte activation and fertilization, with some ICSI cycles constantly facing low fertilization if not complete fertilization failure. Both the sperm and the oocyte play an important role in oocyte activation and in case of physiological activation problems any artificial increase in ooplasmic calcium could rescue fertilization (irrespective of the causative gamete). Such an artificial Ca2+ increase can be achieved using modified ICSI techniques, piezoelectrical manipulation or chemical stimuli. Among these approaches the latter is currently the most accepted in IVF laboratories around the world, and the Ca2+-ionophores calcimycin and ionomycin are among the most extensively studied substances. Available neonatal and neurodevelopmental data indicate that ionophores can be considered safe, which may explain the steadily growing range of indications for application. However, as artificial oocyte activation rarely results in physiological Ca2+ oscillations and is not beneficial for all patients with a suspected activation deficiency, these techniques should not be used without profound indication.

  • Cysteamine, glutathione and ionomycin treatments improve in vitro fertilization of prepubertal goat oocytes

  • Aixa Urdaneta, Ana Raquel Jiménez, María-Teresa Paramio, Dolors Izquierdo
  • Journal:
    Zygote / Volume 12 / Issue 4 / November 2004
    Published online by Cambridge University Press:
    04 February 2005, pp. 277-284

  • The aim of this study was to improve in vitro embryo development of prepubertal goat oocytes by studying the effect of adding cysteamine to in vitro maturation medium, glutathione (GSH) to in vitro fertilization medium and ionomycin to the sperm capacitation medium. In experiment 1, we analysed the effect of 1 mM GSH added to fertilization medium of oocytes matured with 400 μM cysteamine. The control group were oocytes without cysteamine and GSH. In experiment 2, oocytes matured and fertilized in the presence of 400 μM cysteamine and 1 mM GSH, respectively, were inseminated with spermatozoa treated with ionomycin or heparin. In experiment 1, the percentages of total and normal fertilized oocytes were significantly higher for oocytes supplemented with cysteamine and GSH (40.26% and 30.20%, respectively) than for oocytes from the control group (16.66%, and 10.61%, respectively). The percentage of total embryos obtained after 7 days of culture was significantly higher in the group supplemented with cysteamine and GSH (30.62%) than in the control group (8.09%) . In experiment 2, percentages of total and normal fertilized oocytes were significantly higher for the group of spermatozoa capacitated with ionomycin (52.21% and 37.17%, respectively) than with heparin (38.62% and 28.35%, respectively). After 7 days of culture, total embryo rate was significantly higher in the group of sperm capacitated with ionomycin (44.91%) than with heparin (38.69%) . However, the percentage of embryos developed to the blastocyst stage was not affected by any of the treatments studied.