Cantor’s first set theory paper (1874) establishes the uncountability of ${\mathbb R}$ . We study this most basic mathematical fact formulated in the language of higher-order arithmetic. In particular, we investigate the logical and computational properties of ${\mathsf {NIN}}$ (resp. ${\mathsf {NBI}}$ ), i.e., the third-order statement there is no injection resp. bijection from  $[0,1]$ to ${\mathbb N}$ . Working in Kohlenbach’s higher-order Reverse Mathematics, we show that ${\mathsf {NIN}}$ and ${\mathsf {NBI}}$ are hard to prove in terms of (conventional) comprehension axioms, while many basic theorems, like Arzelà’s convergence theorem for the Riemann integral (1885), are shown to imply ${\mathsf {NIN}}$ and/or ${\mathsf {NBI}}$ . Working in Kleene’s higher-order computability theory based on S1–S9, we show that the following fourth-order process based on ${\mathsf {NIN}}$ is similarly hard to compute: for a given $[0,1]\rightarrow {\mathbb N}$ -function, find reals in the unit interval that map to the same natural number.