Herbicide-resistant (HR) kochia is a growing problem in the Great Plains region of Canada and the United States. Resistance to up to four herbicide sites of action, including photosystem II inhibitors, acetolactate synthase inhibitors, synthetic auxins, and the 5-enolpyruvylshikimate-3-phosphate synthase inhibitor glyphosate have been reported in many areas of this region. Despite being present in the United States since 1993/1994, auxinic-HR kochia is a recent and growing phenomenon in Canada. This study was designed to characterize 1) the level of resistance and 2) patterns of cross-resistance to dicamba and fluroxypyr in 12 putative auxinic-HR kochia populations from western Canada. The incidence of dicamba-resistant individuals ranged among populations from 0% to 85%, while fluroxypyr-resistant individuals ranged from 0% to 45%. In whole-plant dose-response bioassays, the populations exhibited up to 6.5-fold resistance to dicamba and up to 51.5-fold resistance to fluroxypyr based on visible injury 28 d after application. Based on plant survival estimates, the populations exhibited up to 3.7-fold resistance to dicamba and up to 72.5-fold resistance to fluroxypyr. Multiple patterns of synthetic auxin resistance were observed, in which one population from Cypress County, Alberta, was resistant to dicamba but not fluroxypyr, whereas another from Rocky View County, Alberta, was resistant to fluroxypyr but not dicamba based on single-dose population screening and dose-response bioassays. These results suggest that multiple mechanisms may confer resistance to dicamba and/or fluroxypyr in Canadian kochia populations. Further research is warranted to determine these mechanisms. Farmers are urged to adopt proactive nonchemical weed management practices in an effort to preserve efficacy of the remaining herbicide options available for control of HR kochia.

Weed resistance surveys that monitor the spread of resistant weeds have mainly been conducted through time-consuming, labor-intensive, and destructive greenhouse herbicide screens. As an alternative, we introduce here a nondestructive leaf-disk assay based on chlorophyll fluorescence (Fv/Fm values that measure photosynthetic efficiency) that allows the detection of resistance to both systemic and contact herbicides within ∼48 h. The current study validated the assay for detecting resistance to fomesafen, glyphosate, and dicamba in Palmer amaranth (Amaranthus palmeri S. Watson), waterhemp [Amaranthus tuberculatus (Moq.) Sauer], kochia [Bassia scoparia (L.) A.J. Scott], and goosegrass [Eleusine indica (L.) Gaertn.]. Negative correlation between Fv/Fm values and spray injury levels was observed in all herbicide–weed combinations at the discriminating doses, except for glyphosate in Amaranthus. The correlation coefficients were −0.41 for fomesafen (10 µM, P < 0.0001) in Amaranthus, −0.92 for glyphosate in E. indica (250 µM, P < 0.0001), and −0.44 for dicamba in B. scoparia (800 µM, P = 0.0023). At the population level, the assay clearly separated susceptible from highly resistant populations. However, the assay showed lower sensitivity in distinguishing populations of different resistance levels or separating populations with low resistance from susceptible populations. At the individual plant level, results from the leaf-disk assay and whole-plant spray tests were concordant in 85.5%, 92.3%, and 71.7% of the plants tested for fomesafen–Amaranthus, glyphosate–Eleusine, and dicamba–Bassia, respectively. The assay yielded 1% to 15% false-positive and 6% to 13% false-negative results across herbicides. The current study demonstrated that the leaf-disk assay is a useful tool to identify weed resistance. Optimization is needed to improve its sensitivities and expand its usage to more diverse herbicide–weed species combinations.

Studies were conducted to determine the usefulness of HOE-39866 (HOE-00661) in chemical fallow systems on the Canadian prairies. HOE-39866 at 0.5 to 1.0 kg ai/ha controlled Russian thistle, kochia, green foxtail, wild oats, and wheat comparable to paraquat, glyphosate, and glyphosate plus the isopropylamine salt of 2,4-D. However, control of barley with HOE-39866 was unacceptable. HOE-39866 was compatible in tank mixtures with ammonium sulfate, paraquat, chlorsulfuron, and metsulfuron. Ammonium sulfate improved weed control when HOE-39866 was applied at 0.25 kg/ha but not at 0.75 kg/ha. Adding paraquat at 0.07 to 0.21 kg ai/ha to HOE-39866 improved control of grass species over HOE-39866 alone. Adding chlorsulfuron and metsulfuron to HOE-39866 provided greater initial control of certain species as well as residual control of many weeds. HOE-39866 alone or in conjunction with other herbicides is an alternative to the herbicides used in chemical fallow systems.

Interference within a mixture of equal densities of common lambsquarters (Chenopodium album L.), kochia [Kochia scoparia (L.) Schrad.], and redroot pigweed (Amaranthus retroflexus L.) in sugarbeets (Beta vulgaris L. ‘Mono Hy D2’) was determined in a 3-yr field study. Yield of sugarbeet roots and sucrose per hectare decreased as intensity of competition from equal populations of these three weeds increased. At densities of 6, 12, 18, and 24 broadleaf weeds per 30 m of row, root yields were reduced 13, 24, 33, and 39%, respectively. Sucrose yields were reduced similarly. Fewer than three weeds per 30 m of row did not significantly reduce root yield. Reduction in root yield (Y) of sugarbeets caused by specific densities (X) of the three broadleaf weeds was predicted by using the linear equation Y = 1.64 + 1.88 X. The actual reductions in yield were always less than the predicted reductions when this equation was tested against 36 weed control systems because the competitive ability of broadleaf weeds that were treated with herbicides, but not killed, was suppressed during the growing season. By harvest, broadleaf weeds present in weed-control-system plots weighed an average of 75 to 85% less than broadleaf weeds present in nontreated plots.

Scanning electron micrographs of spray droplet residual on wheat, sunflower, and kochia indicated that salts and various adjuvants applied with glyphosate affected deposit crystal content, thickness, and contact with the leaf surface. Spray deposits of glyphosate applied with diammonium sulfate contained distinct crystals, which related to enhanced toxicity of glyphosate applied alone, or to overcoming antagonism of glyphosate toxicity by calcium chloride, when applied to wheat. In general, glyphosate applied with antagonistic calcium chloride salt formed spray deposits that were amorphous, thick, and without crystals. Glyphosate sprayed with nonantagonistic diammonium sulfate and ammonium chloride salts had deposits with crystals and an evenly spread residue beneath the crystals. The various micrographs indicate that the antagonism of glyphosate phytotoxicity by salts may be in part from physical entrapment of glyphosate in the spray deposit. Glyphosate spray droplet residue contact with wheat, sunflower, and kochia surfaces related to observed differences in glyphosate toxicity to these species.

High herbicide costs and uncertainty about annual weed control at planting have limited adoption of no-till spring wheat production systems in the northern Great Plains. Chlorsulfuron, metsulfuron, and CGA-131036 at 10 to 20 g ai ha–1 plus nonionic surfactant generally controlled both emerged kochia and wild mustard equally well (>80%) whether or not combined with glyphosate at 250 g ha–1 plus nonionic surfactant. In two of three trials persistent phytotoxic residues of these sulfonylurea herbicides in soil controlled both weeds better in midseason and early summer 1 yr after treatment than did glyphosate, which has only foliar activity. While the absolute net returns of different treatments varied among herbicides, relative net returns were insensitive to changes in either herbicide or wheat price. Herbicide use tended to boost net returns for no-till spring wheat in years with good weather but depressed net returns in a drought year. Chlorsulfuron at 10 and 20 g ha–1 increased net returns in all three trials. Metsulfuron and combinations of either metsulfuron or chlorsulfuron with glyphosate had variable effects on net returns.

Combinations of thifensulfuron with carbaryl, chlorpyrifos, malathion, and methomyl caused soybean injury that exceeded the expected injury based on effects of herbicide and synergists applied alone. Thifensulfuron-induced injury and growth reductions generally increased as insecticide rates increased from 140 to 560 g ai ha-1. TBPT and fenarimol also synergistically enhanced thifensulfuron injury in soybeans. Yellow foxtail control by thifensulfuron was enhanced slightly by all insecticides except carbaryl. Insecticides did not enhance kochia control by thifensulfuron.

Studies were conducted to determine the effects of tillage level on weed control and yield of asparagus grown on a sandy loam soil under sprinkler irrigation. Without herbicides, horseweed and kochia populations increased in no-till asparagus production over a 3-yr period, compared to asparagus that was rototilled either once in the spring or once in the spring and again at layby. Without herbicides, hairy nightshade populations increased most when asparagus was rototilled both in the spring and at layby. Volunteer asparagus was controlled best when herbicides and tillage were combined in the spring and again at layby. Common groundsel was controlled without herbicides by rototilling both in the spring and at layby. Diuron at 1.4 kg ai/ha plus prodiamine at 1.4 kg/ha or metribuzin at 0.9 kg/ha plus norflurazon at 2 kg/ha controlled all weeds under all three tillage levels when split-applied in the spring and at layby. Tillage level had no effect on total asparagus spear number or weight if tillage was performed in mid March before asparagus emergence.

Management systems for direct-seeded and transplanted sugarbeets (Beta vulgaris L. ‘Mono Hy D2′) were compared for weed control and sugarbeet selectivity from 1983 through 1985 in western Nebraska. Broadleaf weed density was similar, but yellow foxtail [Setaria glauca (L.) Beauv. # SETLU] density was lower in transplanted compared to direct-seeded sugarbeets. Preplant soil-incorporated applications of cycloate (S-ethyl cyclohexylethylcarbamothioate) plus trifluralin [2,6-dinitro-N,N-dipropyl-4-(trifluoromethyl)benzenamine] at 3.3 plus 0.6 kg ai/ha or ethofumesate [(±)-2-ethoxy-2,3-dihydro-3,3-dimethyl-5-benzofuranyl methanesulfonate] plus trifluralin at 2.2 plus 0.6 kg/ha was noninjurious to transplanted sugarbeets but caused severe injury to direct-seeded sugarbeets. The combination of cycloate or ethofumesate with trifluralin improved weed control over that obtained when cycloate or ethofumesate was used alone. By combining the improved weed control obtained from cycloate plus trifluralin or ethofumesate plus trifluralin with the transplanting crop establishment technique, a superior sugarbeet weed control program was developed.

An experiment was initiated in 1970 and continued through 1979 by exhuming and germinating seed of 12 economic weed species buried beneath 23 cm of soil in eastern and western Nebraska. Loss in germination of exhumed seeds over years is mathematically characterized by the formula for the rectangular hyperbola, which represents many shapes of curves that have zero as their lower limit. Of the 12 weed species, only fall panicum (Panicum dichotomiflorum Michx.) and redroot pigweed (Amaranthus retroflexus L.) seed germination did not drop significantly over the 10-yr burial period. Germination of redroot pigweed seed was higher when buried in eastern Nebraska, but was higher for smooth groundcherry (Physalis subglabrata Mack&Bush.) and velvetleaf (Abutilon theophrasti Medic.) when buried in western Nebraska. Germination of the other nine species were not affected by burial location. The 12 weed species can be ranked as those showing most to least rapid loss of germination during burial for 10 yr as follows: honeyvine milkweed [Ampelamus albidus (Nutt.) Britt.], hemp dogbane (Apocynum cannabinum L.), kochia [Kochia scoparia (L.) Schrad.], sunflower (Helianthus annum L.), large crabgrass [Digitaria sanguinalis (L.) Scop.], common milkweed (Asclepias syriaca L.), musk thistle (Carduus nutans L.), velvetleaf, fall panicum, redroot pigweed, green foxtail [Setaria viridis (L.) Beauv.], and smooth groundcherry.

An in vivo ALS assay was used to differentiate between susceptible and resistant kochia biotypes from Idaho and Montana. Experiments were also conducted using this assay to determine the effect of the timing of herbicide application on ALS activity in resistant and susceptible cocklebur biotypes from Mississippi. When treated with chlorsulfuron, resistant kochia biotypes demonstrated 160-and 170-fold resistance at the whole plant level for the Idaho and Montana biotypes, respectively, compared to susceptible biotypes. Using the in vivo ALS assay, the Idaho and Montana biotypes showed 70- and 490-fold resistance, respectively, based on I50 values. The biotypes also demonstrated a low level of cross-resistance to imazethapyr using the in vivo technique. Resistant cocklebur biotypes showed approximately 200-fold resistance to imazaquin compared with susceptible biotypes. This research demonstrated that the in vivo assay can be used effectively to identify resistant biotypes 6 and 24 h after treatment, and to determine the extent of cross-resistance.

From 1976 to 1981, emergence date, height, and dry weight per plant were taken for eight weed species grown at Bushland, TX, at two stand densities. Height and dry weight per plant were related to precipitation and degree-days for air and soil temperatures using base temperatures of 10, 15.5, or 21 C. Kochia [Kochia scoparia (L.) Schrad. ♯ KCHSC] emerged early in March and was followed by poison suckleya [Suckleya suckleyana (Torr.) Rydb. ♯ SUKSU], sunflower (Helianthus annuus L. ♯ HELAN), and common cocklebur (Xanthium pensylvanicum Wallr. ♯ XANPE) in early April. Buffalobu(Solanum rostratum Dunal. ♯ SOLCU), puncturevine (Tribulus terrestris L. ♯ TRBTE), lanceleaf sage (Salvia reflexa Hornem. ♯SALRE), and barnyardgrass (Echinochloa crus-galli L. Beauv. ♯ECHCG) emerged in late April. The weeds began to increase in height during May but plants did not start increasing in weight until June. Kochia, common cocklebur, and sunflower grew tallest, produced most dry matter, made most efficient use of water, and weighed most per plant. Heat unit accumulations were strongly correlated with plant height.

Clomazone is used in the Central Great Plains for weed control during fallow in a winter wheat-fallow rotation. Improved precipitation storage during non-crop periods has stimulated new crop rotations such as winter wheat-corn or proso millet-fallow. The objective of this study was to determine if clomazone applied in the fall after winter wheat harvest would injure succeeding spring-planted crops. Greenhouse studies indicated crop tolerance to clomazone was in the order of safflower > corn > proso millet > barley > winter wheat. Clomazone did not affect grain yields of safflower, corn, or proso millet grown at two field sites with different soil textures (silt loam and sandy loam), nor germination of seed from treated plants of these crops. A no-till production system with clomazone increased grain yields for all crops compared to the conventional system where tillage replaced clomazone for fallow weed control.

Field studies were conducted to evaluate potato injury and weed control with postemergence-directed (PDIR) bromoxynil, glufosinate, or monocarbamide dihydrogen sulfate (MCDS). All three herbicides controlled hairy nightshade, common lambsquarters, redroot pigweed, and kochia ≥ 85%, depending on rate, with either mid- or late-PDIR treatment. Bromoxynil or MCDS caused 5 to 6% more initial injury with mid- than with late-PDIR treatment, but total yield and yield of U.S. #1 tubers were not reduced by either herbicide. PDIR glufosinate caused moderate injury at 0.84 kg ha−1, the rate needed for acceptable (≥ 85%) weed control. Total yield was reduced 10% and U.S. #1 yield was reduced 30% by PDIR glufosinate at 0.84 kg ha−1. Thus bromoxynil and MCDS showed potential for use as PDIR treatments in potatoes, but glufosinate did not.

Ten herbicide treatments were evaluated for early season control of three broadleaf weeds and effects on marketable potato yields in low-organic, coarse-textured soil. All treatments controlled prostrate pigweed 100%. Trifluralin with metolachlor or with EPTC did not control kochia well. Pendimethalin alone or with EPTC controlled Russian thistle poorly, and produced the lowest marketable tuber yields. Fluorochloridone at 0.6 kg ai/ha caused chlorosis and reduced potato yield 11%. Treatments with metribuzin tended to have high potato yields.

Greenhouse studies determined the dose-responses of cheat, downy brome, Japanese brome, jointed goatgrass, and winter wheat to preplant-incorporated MON 37500 and its residual effects on kochia. Concentrations of MON 37500 up to 60 ppbw did not affect winter wheat. MON 37500 did not prevent weed emergence, but increasingly inhibited weed growth as the dose was increased up to about 20 ppbw. GR50 values were 16, 16, 11, and 31 ppbw for cheat, downy brome, Japanese brome, and jointed goatgrass, respectively. Japanese brome was more susceptible than cheat or downy brome, and jointed goatgrass tolerated two to three times more MON 37500 than the Bromus species. Plant dry weights of kochia seeded after removal of the winter annual grasses decreased with increasing initial MON 37500 concentrations up to 20 ppbw. Kochia density was influenced by which winter annual grass was grown previously.

Field experiments were conducted to study the feasibility of applying glyphosate [N-(phosphonomethyl) glycine] postemergence with a recirculating sprayer and a vertical roller for the control of common lambsquarters (Chenopodium album L.), common sunflower (Helianthus annuus L.), kochia [Kochia scoparia (L.) Schrad.], redroot pigweed (Amaranthus retroflexus L.), and velvetleaf (Abutilon theophrasti Medic.) in sugarbeets (Beta vulgaris L.). Glyphosate was applied twice each year at 1.7 kg/ha with a recirculating sprayer in 1977 and 1978, or twice as a 20% (v/v) solution with a vertical-roller applicator in 1979. By harvest, 70 to 74% of the total population of treated common sunflower, kochia, and redroot pigweed, 61% of the common lambsquarters, and 30% of the velvetleaf was dead. Root yields in glyphosate-treated plots, when averaged over 3 yr, were increased 5800, 8500, 12 500, and 13400 kg/ha at densities of 6, 12, 18, and 24 broadleaf weeds (equal densities of common lambsquarters, kochia, and redroot pigweed)/30m of row, respectively. Where equal densities of common sunflower and velvetleaf competed with sugarbeets, root yields in glyphosate-treated plots, when averaged over 2 yr, were increased 4400, 11900, 11700, and 10700 kg/ha, respectively, at these same densities.

Field experiments were conducted near Scottsbluff, NE, to evaluate the selectivity of sequential POST applications of ethofumesate and triflusulfuron for weed control in sugarbeet. When applied alone ethofumesate and triflusulfuron reduced common lambsquarters, hairy nightshade, and redroot pigweed density while triflusulfuron also reduced kochia density compared with an untreated control. Combining ethofumesate with desmedipham plus phenmedipham increased sugarbeet injury compared with desmedipham plus phenmedipham. Combining triflusulfuron with desmedipham plus phenmedipham increased kochia control but did not increase sugarbeet injury over that obtained with desmedipham plus phenmedipham. Weed control and sugarbeet injury from herbicides generally were unaffected by application in 97 or 182 L/ha of water carrier. However, herbicide control of hairy nightshade in 1993 was greater with 97 than 182 L/ha of water carrier.

The first significant cases of herbicide-resistant weed populations were to the triazines in the 1970s. In the last 10 years there has been an increase in the number of weed populations that have become resistant to an array of herbicides. In some of these cases, like rigid ryegrass in Australia, a multitude of resistant biotypes has evolved with different mechanisms of resistance. If the present trend continues, the number of herbicides effective on certain weed species may diminish rapidly. To counteract this trend, industry has organized a number of intercompany working groups to specifically address the development of resistance and to implement plans to manage resistance. University and extension along with industry personnel across the world have begun educating growers on resistance management. However, this effort needs to be intensified to find new solutions for controlling weeds through the use of integrated weed management practices that incorporate new and established herbicides with cultural, mechanical, and biological control methods. The challenge is to develop cost effective, environmentally sustainable programs for weed control while maintaining the present efficiency in food and fiber production so that needs of an ever expanding human population can be met.

Glasshouse and growth chamber experiments were conducted to determine chlorsulfuron {2-chloro-N-[[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino] carbonyl] benzenesulfonamide} phytotoxicity to kochia [Kochia scoparia (L.) Schrad. ♯ KCHSC] and green foxtail [Setaria viridis (L.) Beauv. ♯ SETVI] as influenced by temperature and humidity for 1 week after treatment, and by soil moisture and nitrogen. Chlorsulfuron was more phytotoxic to both kochia and green foxtail at 95 to 100% than at 45 to 50% relative humidity for 1 week after treatment whether at 10, 20, or 30 C. Chlorsulfuron phytotoxicity was similar with all posttreatment temperatures at each humidity, except that phytotoxicity was lower at 30 C than at 10 or 20 C at 90 to 100% humidity. Surfactant added to chlorsulfuron exhanced control of kochia more than that of green foxtail and overcame temperature and humidity effects on chlorsulfuron phytotoxicity. A simulated rainfall of 2 mm after chlorsulfuron application reduced toxicity of chlorsulfuron to green foxtail more than to kochia. Chlorsulfuron was more phytotoxic to green foxtail growing with a high than a low soil nitrogen level. High soil moisture following chlorsulfuron application enhanced phytotoxicity to kochia and green foxtail compared to high soil moisture before treatment.