Three new calicioid lichens are described from the Neotropics. Mazaediothecium uniseptatum, with 1-septate ascospores, is described from French Guiana. Mycocalicium enterographicola, with turbinate, green-pruinose apothecia and stalk and a distinct mazaedium, occurring lichenicolous on Enterographa cf. quassiaecola Fée, is described from Sergipe State in Brazil. Stenocybe tropica, with 3-septate ascospores remaining clustered in the mouth of the apothecium, and persistent asci, is described from mangrove tree bark in São Paulo State, Brazil.