Well-preserved vertebrate microremains are abundant in the residues from a calcareous grey siltstone in the karawaka biozone (?late Givetian) of the Aztec Siltstone at Mount Crean, Lashly Mountains, Antarctica. Acanthodians are represented by scales of acritolepid Pechoralepis juozasi sp. nov., climatiid Nostolepis sp. cf. N. gaujensis Valiukevičius, diplacanthid Milesacanthus antarctica Young & Burrow, and an undetermined acanthodiform. The acanthodian assemblage resembles those from early Frasnian carbonates of central Iran. Chondrichthyan elements in the fauna include rare teeth of Aztecodus harmsenae Long & Young and Antarctilamna prisca Young, ctenacanthoid-type scales and branchial denticles which are possibly from Antarctilamna, and scales of an indeterminate chondrichthyan. An isolated set of acanthodid acanthodiform jaws from the uppermost ‘phyllolepid’ biozone of the Aztec Siltstone at Mount Ritchie, Warren Range, Antarctica is also described.