Beach filling activities were conducted along the Mar del Plata coastline, Argentina, with sediments dredged from the harbour mouth between November 1998 and April 1999. We determined the percentage of imposex of Olivancillaria deshayesiana before, during and immediately after beach filling. The current situation (2005–2007) of imposex incidence was compared between populations from Mar del Plata and Mar Chiquita, a less impacted area. In addition, we evaluated differences in shell shape and body weight between females with and without imposex. The percentage of imposex was significantly higher during (85.3%) and after (73.9%) beach filling than before it (31.0%). No differences in body weight were found in the female population but shell length was significantly lower in females with imposex. As expected, the Mar Chiquita population showed no signs of imposex. This is the first report of imposex in beaches nourished with sand dredged from the port. Our results highlight the importance of analysing more carefully the sediment before beach filling. On the basis of the high level of imposex recorded near the port of Mar del Plata, O. deshayesiana can be used as a biological indicator of marine contamination with butyltin compounds.