This paper introduces a symmetric bar chart-shape (SBCS) microwave sensor for measuring permittivity of fluidic samples. For designing purposes, the introduced sensor was used based on the field changes between the SBCS and rectangular loop microstrip (RLM) structure. When a sample is placed on the sensing location, interaction between SBCS and RLM varies the field intensity. The vinegar samples were combined with water and then they are placed on the sensor. The change in field intensity changes the resonance frequency. However, there is a relationship between the permittivity of samples and the resonance frequencies. The proposed sensor is implemented on the substrate of RO4003C. The relative permittivity of samples changed from 59 to 77 and the resonance frequencies changed from 2.3 to 1.4 GHz. The quality factor is 3544 and the sensitivity is 2.2%.