When grown without soybean [Glycine max (L.) Merr.] interference, eastern black nightshade (Solanum ptycanthum Dun.) planted in May produced as many as 7000 berries and 800000 seeds per plant. Eastern black nightshade planted in mid-July produced as many as 100 berries, but those planted in mid-August failed to produce berries. Eastern black nightshade produced less than 85 berries per plant when planted with soybeans in May, 0 to 3 berries per plant when planted into soybeans in June, and no berries when planted into soybeans in July. However, when soybeans were defoliated in July to simulate hail injury, eastern black nightshade that had been planted with soybeans in May produced up to 1600 berries per plant, and those planted into the defoliated soybeans in July produced up to 58 berries per plant. We suggest, therefore, that in vigorously growing soybeans, control of eastern black nightshade emerging in the row would only be required through June to prevent berry production. If, however, the soybean canopy were disturbed by hail or other factors, eastern black nightshade seedlings emerging in late July or early August might produce berries by soybean harvest.

The relative sensitivity of various crops and weeds to celery root residue incorporated into soil was determined in greenhouse experiments. A range of concentrations was used to determine the percentage of celery residue needed to cause a 50% reduction in plant growth (I50). The most sensitive weed was spiny amaranth and the least sensitive was common purslane. The most sensitive crop was radish and the least sensitive was celery. Selectivity was calculated as crop I50/weed I50 and varied from a maximum of 13 for celery and spiny amaranth to a low of 0.4 for radish and common purslane. Selective weed suppression might be obtained when celery residue is present.

Spray solutions containing a cationic or a nonionic surfactant were applied to six plant species at a broad range of concentrations. The species investigated were three weeds (black nightshade, chamomile, and quackgrass) and three crops (winter wheat, pea, and tomato). The microroughness of the leaf surface as revealed by scanning electron microscopy appeared to be a relevant retention-determining factor. Plant species with crystalline epicuticular waxes (winter wheat, pea, and quackgrass) retained much less spray solution than the other species, which are characterized by a smooth cuticular surface. The two surfactants enhanced retention on species with a reflective surface, whereas retention on black nightshade, chamomile, and tomato was hardly influenced by addition of surfactants. The two surfactants had a similar influence on the retention. Surfactant at 1% (wt/v) enhanced retention on pea, winter wheat, and quackgrass by factors of twenty, six, and four, respectively, compared with retention without surfactant. A linear relation between retention and logarithm of surfactant concentration was observed. Retention of spray drops was related not to equilibrium surface tension of the spray solution but rather to dynamic surface tension.

The effect of black and eastern black nightshade on the amount of photosynthetically active radiation (PAR) intercepted by a processing tomato canopy was studied along with the correlation between PAR and tomato growth and yield. During 1989 and 1990, black and eastern black nightshade were established at densities of 0 to 4.8 m−2 within rows of transplanted, irrigated processing tomatoes. Increasing the density of either nightshade species decreased the number of tomato fruit; however, eastern black nightshade reduced tomato yield more than black nightshade. Eastern black nightshade was taller than the tomatoes, reducing PAR reaching the top of the tomato canopy. PAR reaching the top of the tomato canopy was positively correlated with yield and negatively correlated with eastern black nightshade density. Eastern black nightshade intraspecific competition decreased both stem and berry weight. Black nightshade was never taller than tomatoes and did not affect PAR reaching the top of the crop canopy. Increasing the density of black nightshade decreased berry dry weight but increased the weight of stems and leaves.

Black nightshade was subjected to two degrees of water stress by adding polyethylene glycol 20,000 (PEG) to the nutrient solution 5 d before treatment with glyphosate. The ED50 values for glyphosate, determined from dose-response curves, demonstrated that both degrees of water stress strongly increased the ED50, with and without the surfactant Ethomeen T/25 in the spray solution. The surfactant reduced the ED50 5-, 4.6-, and 6.9-fold at 0, 15, and 20% PEG, respectively. A 14C study demonstrated that unstressed plants absorbed 22% of applied glyphosate. Without surfactant, water stress reduced foliar absorption 2.2-fold at 15% PEG and 4.5-fold at 20% PEG. With surfactant, the foliar absorption was 35% of the applied amount in unstressed and water-stressed plants. The surfactant and PEG reduced the translocation efficiency of glyphosate. The surfactant had the most pronounced influence and reduced the translocation efficiency 1.5-fold at 0% PEG, 2.2-fold at 15% PEG, and 1.8-fold at 20% PEG. Induction or removal of water stress 24 h after glyphosate treatment indicated that plant growth rate is positively correlated with glyphosate efficacy. It was concluded that the surfactant can overcome the adverse influence of water stress on foliar absorption of glyphosate but not the adverse, postapplication influence of water stress on glyphosate efficacy.

Black nightshade plants were controlled by hoeing in the same cotton plots each year (1982 to 1986) for 3 to 15 weeks after crop emergence to evaluate the influence of several black nightshade-free periods on cotton yield, reproduction of black nightshade, and longevity of weed seeds in soil. Similar information, although limited, was also collected for Palmer amaranth that escaped the initial herbicidal treatment each year. Except for 1982, black nightshade competing with cotton for the duration of the growing season in nonhoed plots severely reduced yields (60 to 100%), with greatest yield reductions (82 to 100%) occurring in 1983 and 1984 when 0.5 to 0.7 cm of rain fell within 10 days after cotton planting. When combined with cultivation, a 3-week nightshade-free period at cotton planting was of sufficient duration to protect cotton yields. Weed seed production for all hoed treatments was less than 1% of the nonhoed treatment, and after five consecutive cotton crops (1982 to 1986), the amount of both black nightshade and Palmer amaranth seeds in soil was similar for all hoed treatments. These populations were 60 to 80% and 95 to 97% less than beginning populations of black nightshade and Palmer amaranth in 1982, respectively. After 5 yr of continuous treatments, cotton was grown in 1987, with standard cultivation as the only method of weed control, to evaluate how the weed-free periods in 1982 to 1986 influenced weed seed populations in the soil and the resultant effect on weed competition and cotton yields. Reduction of cotton yields in 1987, in the absence of weed-free periods, indicated that black nightshade seed survival in soil appears to be sufficiently long for ample establishment of this weed to compete with cotton. Thus, fields will have to be kept weed free for greater than 5 yr to reduce black nightshade populations to a level that will not reduce cotton yields.

The effect of sequential hexane, ethyl acetate, and aqueous methanol extracts of ‘Regal’ sweetpotato periderm on seed germination of sweetpotato, proso millet, and seven weed species was studied. The hexane extract, which contained the nonpolar components of the periderm tissue, was least inhibitory. It inhibited velvetleaf, proso millet, black nightshade, and redroot pigweed germination, and maximum inhibition was 56% for black nightshade at 200 mg of periderm extracted ml–1. The ethyl acetate fraction was inhibitory to proso millet, velvetleaf, black nightshade, goosegrass, tall morningglory, coffee senna, and redroot pigweed. The estimated I50 for ethyl acetate ranged from 17 mg periderm extracted ml–1 for black nightshade to 201 mg ml–1 for coffee senna. Sweetpotato, tall morningglory, and eclipta germination was not inhibited by this extract at the concentrations tested. The aqueous methanol extract was much more inhibitory than the hexane or ethyl acetate extracts, and there was considerable variation between species in response to this extract The I50 estimates for the aqueous methanol extract were 0.5, 0.6, 2.8, 4.4, 5.1, 9.6, 15.7, 21.0, and 25.8 mg ml–1 for velvetleaf, proso millet, black nightshade, goosegrass, sweetpotato, tall morningglory, eclipta, coffee senna, and pigweed, respectively.

The objectives were to compare the growth of black and eastern black nightshade and develop predictive models for their growth. Black and eastern black nightshade were planted on June 3 and 23, and July 12 in 1988 and May 28, June 17, and July 10 in 1989. Exponential models were strongly correlated with the growth of both species across all planting dates. Eastern black nightshade produced more biomass than black nightshade, although weight of berries was equivalent. Leaf area per plant 60 d after planting was five times greater for eastern black nightshade than black nightshade. Eastern black nightshade responded to self-shading by increasing specific leaf area.

Activity, adsorption, mobility, and field persistence of one emulsifiable concentrate (EC) and three microencapsulated (ME) formulations of alachlor were studied with petri dish bioassay, based on root response of oats grown in sand or soil. Both bioassays indicated that activity of all formulations was increased with increasing herbicide concentration. EC-alachlor in sand showed the highest activity, while ME-alachlorL and ME-alachlorA, the lowest; the activity of ME-alachlorC was intermediate. In silty clay loam soil, EC-alachlor had the highest activity, while ME-alachlorL had the lowest; and ME-alachlorA showed intermediate activity and was similar to that of ME-alachlorC More alachlor was adsorbed on the soil or remained encapsulated (not biologically available) after ME-alachlorL and ME-alachorA application compared to EC-alachlor. An intermediate amount of alachlor was adsorbed or remained inside the capsules when the ME-alachlorC formulation was applied. Greater amounts of alachlor were leached through a silty clay loam after EC-alachlor application compared to the three ME-alachlor formulations. Biologically available alachlor was not detected below 15 or 10 cm after application of EC- and ME-alachlor formulations, respectively. All alachlor formulations applied alone or in mixture with atrazine showed similar field persistence. Biologically available alachlor was not detected in the 0- to 10-cm soil depth 30 d after their application. All alachlor formulations applied alone gave excellent control of redroot pigweed and black nightshade, but only partial control of jimsonweed. None of the herbicide treatments showed any detrimental effect on corn, and all of them increased corn yield to the level of weed-free control.

Imazethapyr, alone and in combination with other herbicides, was applied PPI, PRE, and POST to pinto beans to determine weed control and selectivity to the crop. All of the herbicides improved pinto bean yield as compared with the unweeded control. Imazethapyr applied PPI and POST provided excellent control of black nightshade, kochia, Russian thistle, prostrate pigweed, and redroot pigweed. Barnyardgrass control with imazethapyr ranged from 58 to 96% and increased to 98% or greater when imazethapyr was combined with metolachlor, pendimethalin, trifluralin, or EPTC. Pinto bean yield was not reduced from any herbicide treatment compared to the handweeded control.

The interference between dry bean (Phaseolus vulgaris L. var. Red Kidney), black nightshade (Solanum nigrum ♯3 SOLNI), and barnyardgrass (Echinochloa crus-galli ♯ ECHCR) was examined in replacement series experiments. A modified replacement series experiment also was performed to examine the effects of no interference, and intraspecific and interspecific interference on the height, leaf area, and dry weight of bean, black nightshade, and barnyardgrass. In both types of competition experiments, bean germinated earlier than either weed species and caused significant reductions in weed height, leaf area, and dry weight. The impact of bean upon itself was always greater than the effect of either weed species. In laboratory studies, barnyardgrass and black nightshade seeds were subjected to eight concentrations of trifluralin (α,α,α-trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine). Black nightshade was 100 times more tolerant to trifluralin than barnyardgrass. Field studies, conducted at several locations in the Central Valley of California in which trifluralin-treated plots were compared with nontreated plots, demonstrated that trifluralin favors trifluralin-tolerant weeds (black nightshade), but trifluralin-sensitive weeds (barnyardgrass) have not been eliminated despite 20 yr of trifluralin use.

Under irrigated field conditions at Prosser, WA, Russian thistle [Salsola kali (L.) var. tenuifolia Tausch. ♯3 SASKR] began to emerge by mid-March each year and usually had completed its emergence by May 1. Common lambsquarters (Chenopodium album L. ♯ CHEAL) was the next to appear, usually during the third or fourth week of March. Barnyardgrass [Echinochloa crus-galli (L.) Beauv. ♯ ECHCG], redroot pigweed (Amaranthus retroflexus L. ♯ AMARE), hairy nightshade (Solarium sarrachoides Sendt. ♯ SOLSA), black nightshade (5. nigrum L. ♯ SOLNI), eastern black nightshade (S. ptycanthum Dun.), and cutleaf nightshade (S. triflorum Nutt. ♯ SOLTR) generally began to emerge during the first 2 weeks of April and emergence generally peaked mid-April to mid-May. Russian thistle and cutleaf nightshade had the most restricted emergence patterns, whereas seedlings of common lambsquarters, redroot pigweed, hairy nightshade, and black nightshade emerged each month from April through September. Shallow tillage at monthly intervals increased the overall emergence of common lambsquarters, redroot pigweed, black nightshade, and eastern black nightshade; decreased the emergence of barnyardgrass; and had no effect on the emergence of Russian thistle, cutleaf nightshade, or hairy nightshade.

Eight accessions of weedy nightshades representing five species were evaluated for response to four herbicides applied preemergence in growth chamber studies and four herbicides applied postemergence in greenhouse studies. Species differed in susceptibility to all herbicides evaluated. The largest differences were found with postemergence applications of chlorsulfuron {2-chloro-N-[[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl]benzenesulfonamide}; hairy nightshade (Solanum sarrachoides Sendt. ♯ SOLSA) was relatively susceptible, but eastern black nightshade (S. ptycanthum Dun.) was very tolerant. S. ptycanthum, however, was one of the more susceptible species. It was always more susceptible than black nightshade (S. nigrum L. ♯ SOLNI) to all other herbicides evaluated.

Photoacoustic spectroscopy was used to study effects of atrazine and diuron on excised leaves of triazine-susceptible (S) and -resistant (R) biotypes of black nightshade. Changes of oxygen and photothermal components were compared to photochemical fluorescence quenching obtained by fluorimetry. After 1 h incubation in an aqueous solution of atrazine (0 to 200 μM), oxygen component of the photoacoustic signal was strongly decreased in the S biotype while the R biotype was not affected. Also, reoxidation of the primary quinone acceptor (QA−) of photosystem (PS) II of the S biotype was lower than that of the R biotype. With diuron treatments, changes in the characteristics of these biophysical signals were the same in both R and S biotypes. Both oxygen component and photochemical fluorescence quenching were decreased in treated leaves of the R and S biotypes. By using modulated oxygen and heat emissions, and the ratio of the initial inflection point (I) to the fluorescence maximum (P) as herbicide bioassay indicators, we showed that the photoacoustic spectroscopy was also a reliable technique for whole plant studies. Inhibition of photosynthesis was maximal 2 d after onset of treatment with atrazine (200 μM). Inhibitors of PSII did not induce a significant increase of heat emission in leaves which otherwise showed phytotoxic symptoms after treatment By using the photoacoustic technique, it was possible to obtain useful information on photosynthetic activity under herbicide stress, suggesting that pulsed oxygen emitted by leaves could be used to quantify susceptibility or to detect resistance to many types of photosynthetic inhibitors in weeds and crop plants.

A 4-yr study (1985, 1987, 1988, 1989) was conducted on the same field plots at Shafter, CA to evaluate the efficacy of prometryn in controlling black nightshade on planting beds of cotton. Two rates (1.7 and 2.2 kg ai ha–1) were applied at two times (mid March before the preplant irrigation and early April at cotton planting) each year. Incorporation of prometryn into moist planting beds with a powered rotary tiller operated at 10 cm deeper resulted in excellent control of black nightshade under low to moderate weed pressure in 1985 and 1987. Control of nightshade with early and late applications of 1.7 kg ha–1 of prometryn under high weed pressure in 1988 was only 70% at harvest, and yield losses of cotton averaged 25%. Yields of cotton treated with 2.2 kg ha–1 of prometryn in 1988 were not significantly different from weed-free plots. Only the late application of 2.2 kg ha–1 of prometryn prevented significant cotton losses under extreme weed pressure in 1989. Cotton yield losses with the other prometryn treatments ranged from 78 to 100%. Losses of cotton in weedy-check plots that received only cultivation ranged from 22% in the absence of rain or irrigation at cotton planting in 1987 to as much as 100% when rain fell in 1988 or plots were irrigated at planting in 1989. Plots hoed one time 4 wk after cotton planting yielded an average of 84% as much seed cotton as weed-free plots. In an attempt to determine why the efficacy of prometryn declined between 1985 and 1989, several experiments were conducted in 1988 to 1990 to discover reasons for this poor control of nightshade. Because efforts failed to provide evidence for the movement of the herbicide with water, the development of weed resistance to prometryn, or accelerated degradation of this herbicide in soil, increasing weed seed populations in soil were believed to have contributed greatly to the declining nightshade control from prometryn. The fact that prometryn applied and incorporated into flat soil provided excellent control of nightshade in 1990 under sprinkler irrigation indicated that both soil moisture and incorporation techniques limited activity of prometryn in planting beds in 1988 and 1989. Incomplete control of nightshade plus good soil moisture at planting contributed to the high weed populations in 1988 and 1989.

Field studies were conducted in 1990 and 1991 at Davis and Five Points, CA to evaluate weed control with napropamide and hand-weeding and the effect on yield and profitability in transplanted bell pepper. Weed cover 8 wk after transplanting averaged less than 10% in plots hand-weeded biweekly for 6 wk after transplanting. Weed cover at harvest was less than 30% in plots hand-weeded at 8 wk after transplanting. Napropamide treatment reduced weed cover at 8 wk after transplanting an average of 34% compared to untreated plots, but differences declined to 14% at harvest. Time required to hand-weed plots was greater if the interval between weeding was 4 instead of 2 wk. The time needed to hand-weed plots was reduced from 38% to 71% by napropamide treatment. When weeds were excluded by hand-weeding for the entire season, bell pepper yielded 25 810 kg/ha and was 4% to 18% higher than other treatments. Net returns were greater for plots that were hand-weeded the entire season or when napropamide was combined with hand-weeding at 4 and 8 wk after transplanting, compared to other treatments.

Black nightshade was selectively controlled along with other annual weeds in direct-seeded tomatoes by BAY SMY 1500 applied preemergence. BAY SMY 1500 was also found selective for transplanted tomatoes when applied prior to transplanting or soon after planting prior to black nightshade emergence. Black nightshade control and crop selectivity were obtained with BAY SMY 1500 at 0.75 and 1.25 kg ai ha-1 applied on sandy loam and clay loam soils, respectively.

In field experiments at Prosser, WA, black nightshade (Solanum nigrum L. # SOLNI), eastern black nightshade (S. ptycanthum Dun. # SOLPT), American black nightshade (S. americanum Mill. # SOLAM), hairy nightshade (S. sarrachoides Sendtner # SOLSA), and sometimes accessions within a species responded differentially to herbicides applied preplant incorporated, preemergence, or postemergence. Also, the response of the nightshades to some of the herbicides varied significantly between years. Generally, black nightshade was more tolerant of herbicides than were the other three species. An accession of black nightshade from England and an accession of eastern black nightshade from Minnesota were the most tolerant of herbicides of eight accessions evaluated. Of the 25 herbicides evaluated, preplant-incorporated applications of ethalfluralin [N-ethyl-N-(2-methyl-2-propenyl)-2,6-dinitro-4-(trifluoromethyl) benzenamine] and preemergence applications of alachlor [2-chloro-N-(2,6-diethylphenyl)-N-(methoxymethyl)acetamide], chloramben (3-amino-2,5-dichlorobenzoic acid), lactofen [1′-(carboethoxy)ethyl 5-[2-chloro-4-(trifluoromethyl) phenoxy]-2-nitrobenzoate], and metolachlor [2-chloro-N-(2-ethyl-6-methylphenyl)-N-(2-methoxy-1-methylethyl] acetamide] controlled all species best. These herbicides reduced the oven-dry weights of both accessions of all four species of nightshades by 93% or more. Nightshades varied widely in their response to the thiocarbamates.