Increasing the stability and reliability of navigation for mobile objects of different classes is becoming increasingly significant. Satellite navigation systems have a fundamental defect: a vulnerability to hacking and spoofing. Observation of the horizon line is significant for two applications. The first is stellar inertial navigation systems. In this case, the horizon line can be used for local vertical estimation. Errors in local vertical estimation directly affect coordinate errors. The second is correlation-extremal navigation based on the observed horizon line shape (when islands or continents are observed from aerial vehicles). In both cases, instability of the horizon line produces navigation errors. A measurement procedure for horizon line position estimation was proposed and realised. Around-the-clock horizon line shooting was undertaken in 2013. Processing of the results shows a horizon line direction instability of about 5–7 angular minutes during the day time.