Management of perennial weeds is a major concern in reduced-tillage cropping systems. Field research was conducted at Nashua, IA, from 1977 through 1990 to evaluate the long-term impacts of tillage and cropping patterns on perennial weed populations in corn and soybean production. Continuous corn and a corn/soybean rotation were conducted utilizing moldboard plow, chisel plow, ridge tillage, and no-tillage systems. The research area was free of established perennial weed species at the initiation of the experiment in 1977. Hemp dogbane was observed by 1980, with the greatest densities in no-tillage. By 1990, continuous corn had greater hemp dogbane densities with no-tillage than other tillage system by crop rotation treatments. American germander densities were not affected by tillage systems in 1980 and 1981, but by 1990, corn/soybean rotations had greater densities in moldboard plow than other tillage systems. Field bindweed developed primarily in the corn/soybean rotations with the greatest densities occurring in no-tillage. Greater and more diverse populations of perennial weeds developed in reduced-tillage systems than in the moldboard plow system. However, practices used to control annual weeds and environmental factors interacted with tillage to regulate perennial weed populations.