This is a registered report for a study of racial and ethnic variation in the relationship between negativity bias and political attitudes. Pioneering work on the psychological and biological roots of political orientation has suggested that political conservatism is driven in large part by enhanced negativity bias. This work has been criticized on several theoretical fronts, and recent replication attempts have failed. To dig deeper into the contours of when (and among whom) negativity bias predicts conservatism, we investigate a surprisingly overlooked factor in existing literature: race and ethnicity. We propose that political issues represent threat or disgust in different ways depending on one’s race and ethnicity. We recruited 174 White, Latinx, and Asian American individuals (in equal numbers) to examine how the relationship between negativity bias and political orientation varies by race/ethnicity across four domains: policing/criminal justice, immigration, economic redistribution, and religious social conservatism.