An investigation was conducted into the effects of increasing plasma prolactin concentration with long photoperiod or suppressing it with bromocryptine on the fertility of ewe lambs. One hundred and twenty dim Forest ewe lambs were divided into three groups: group C was kept as a control; group L was exposed to a long photoperiod (16 h light: 8 h dark) and group B was given daily injections of 1 mg bromocryptine from 12 days before until 21 days after mating. All three groups were mated in late November, 30 days after housing, following progestagen/PMSG treatment. Post-mating prolactin concentrations were significantly higher in group L and lower in group B compared with group C (P > 0·05). There was a tendency for pregnancy rate and the number of corpora lutea to be represented by viable foetuses to be lower in group L than in group C (P < 0·05). Furthermore, post-mating progesterone concentration was higher in this group compared with those in group C (P > 0·05). In a second experiment, 100 Welsh Χ Texel ewe lambs were given treatment C or L. Embryo survival tended to be lower and crown rump length, embryo weight and amniotic sac width were reduced at 25 days post mating in the animals given treatment L, compared with those given treatment C (P < 0·05). The combined results of the experiments indicated that fewer corpora lutea were represented by viable embryos or foetuses in group L than in group C (P < 0·05). These results suggest that embryo growth and survival is reduced in animals bred under a long photoperiod.

The effect of bromocryptine on milk production was investigated in 32 crossbred ewes (Flemish Milksheep х Suffolk) with one sucking lamb, during lactation and during drying off. After weaning the lambs, all ewes were hand milked twice daily for 3 days. From day 4 onwards, the ewes were divided into four groups with equal mean daily milk production. Ewes of groups A and B were hand milked twice daily during the following 9 days. Ewes of groups C and D were dried off by reducing the milking frequency. They were milked on days 5, 8 and 12. In groups B and D, bromocryptin (1 ml of 1 g/l ethanol 50%) was injected intramuscularly twice daily while control ewes (groups A and C) received 1 ml ethanol 50%. Blood samples were taken by venipuncture prior to each injection and were assayed for prolactin, growth hormone, thyrotropin and cortisol.

Bromocryptine decreased plasma prolactin levels but did not alter growth hormone or cortisol concentrations during lactation or drying off. Thyrotropin levels were elevated by bromocryptine during drying off but were unaffected during galactopoiesis.

During lactation the daily milk production remained unchanged in control ewes while in bromocryptine-treated ewes a proportional decrease of 0·5 was observed. In all ewes the decrease in milk production occurring at drying off could not be accelerated or stimulated by bromocryptine.

It is concluded that prolactin plays an important role in galactopoiesis in ewes but its role is comparatively unimportant compared with other factors during mammary involution.