Weeds represent one of the most important biotic threats to agricultural plant health, and the potential global impact of weeds on crop yields is similar to that of all other pests (animal pests and pathogens) combined. Canola is the most-grown crop in Canada based on seeded area and generates on average Can$29.9 billion in economic activity each year. The objective of this report, sponsored by the Weed Science Society of America Weed Loss Committee, was to provide an updated estimate of potential yield and monetary losses due to weed interference in spring canola grown in Canada and the United States. Quantitative yield data from field experiments were provided by researchers and weed science professionals in the northern Great Plains region; the major canola-producing area of North America. Overall, 89 yield loss estimates were compiled, covering the 18-yr period from 2003 to 2020. Average canola yield losses due to weed interference in Alberta, Saskatchewan, Manitoba, and North Dakota were 35%, 30%, 18%, and 28%, respectively. Potential yield losses weighted by canola harvested area averaged 30%, 28%, and 30% for Canada, the United States, and both countries combined, respectively. Therefore, unfettered weed interference in spring canola represents a potential monetary loss of Can$2.21 billion, $0.16 billion, and $2.37 billion for farmers in Canada, the United States, and both countries combined. The realization of such losses could manifest through continued selection for herbicide-resistant weeds, indicating the critical need for canola farmers to diversify resistance selection pressures by implementing proactive integrated weed management programs.