Lake Vela is a shallow lake facing eutrophication, like many other freshwater reservoirs. A reduction in biodiversity has been observed and cyanobacterial blooms are frequent, occasionally leading to large fish kills. An annual sampling cycle was carried out in the present study, with the aim of understanding the present seasonal variation of Lake Vela’s physico-chemistry and cladoceran assemblage. The present results were compared with those obtained in previous studies. Presently, Lake Vela is facing higher nutrient and Chl a levels than in past years. The cladoceran assemblage is now dominated by small-bodied forms, such as Chydoridae and Bosmina longirostris, except in the spring peak of Daphnia longispina. Still, the seasonal pattern of the cladoceran community is more or less coherent with the general model observed in other temperate lakes. The results also suggest that this was a rather unusual year, with a Daphnia summer peak, probably because of a large fish kill that occurred in late spring.