African women members of the Mothers’ Union in South Africa have forged a neo-indigenous expression of Christianity best expressed in the characteristics of the manyano movement (women’s prayer groups) which include extempore prayer and preaching, extensive fundraising, and the wearing of a church uniform. These women had to resist the restrictions placed upon them by women missionaries and church leadership from England, which included the abolishment of the church uniform during the 1950s. The article traces their struggle of resistance during this period and shows how they fought to wear a uniform and so identified themselves with the movement of women’s prayer unions existing in other churches. It also addresses the significance of the uniform as identified by elderly women from Vulindlela, KwaZulu-Natal, South Africa and suggests some of the existing ambiguities of the church uniform in the current church context.