The Maršíkov–Schinderhübel III pegmatite in the Hrubý Jeseník Mountains, Silesian Domain, Czech Republic, is a classic example of chrysoberyl-bearing LCT granitic pegmatite of beryl–columbite subtype. This thin pegmatite dyke, (up to 1 m in thickness in biotite–amphibole gneiss is characterised by symmetrical internal zoning. Tabular and prismatic chrysoberyl crystals (≤3 cm) occur typically in the intermediate albite-rich unit and rarely in the quartz core. Chrysoberyl microtextures are quite complex; their crystals are irregularly patchy, concentric or fine oscillatory zoned with large variations in Fe content (1.1–5.3 wt.% Fe2O3; ≤0.09 apfu). Chrysoberyl compositions reveal dominant Fe3+ = Al3+ and minor Fe2+ + Ti4+ = 2(Al, Fe)3+ substitution mechanisms in the octahedral sites. Tin, Ga, and V (determined by LA-ICP-MS) are characteristic trace elements incorporated in the chrysoberyl structure, whereas anomalously high Ta and Nb concentrations (thousands ppm) in chrysoberyl are probably caused by nano- to micro-inclusions of Nb–Ta oxide minerals; especially columbite–tantalite. Textural relationships between associated minerals, distinct schistosity of the pegmatite parallel to the host gneiss foliation and fragmentation of the pegmatite body into blocks as a result of superimposed stress are clear evidence for deformation and metamorphic overprinting of the pegmatite. Primary magmatic beryl, albite and muscovite were transformed to chrysoberyl, fibrolitic sillimanite, secondary quartz and muscovite during a high-temperature (~600°C) and medium-pressure (~250–500 MPa) prograde metamorphic stage under amphibolite-facies conditions. A subsequent retrograde, low-temperature (~200–500°C) and pressure (≤250 MPa) metamorphic stage resulted in the local alteration of chrysoberyl to secondary Fe,Na-rich beryl, euclase, bertrandite and late muscovite.