Stenoponia tripectinata tripectinata (Tiraboschi, 1902) is the most prevalent subspecies, within the genus Stenoponia, in the Mediterranean area. This rodent flea is widely distributed throughout southwestern Europe and the North of Africa including Mediterranean islands and the Canary Islands. Nevertheless, from a taxonomical and systematic point, this flea group has been neglected over the years. Therefore, the aim of this study was to carry out a comparative morphometric, phylogenetic, and molecular study of two populations of S. t. tripectinata isolated from rodents collected from different islands from the Canary Archipelago and from Corsica to clarify the taxonomic status of these two isolated populations and to assess the morphological and molecular differentiation between them. For this purpose, we have analyzed several morphological traits and sequenced five molecular markers (EF1-α, ITS2, cox1, cox2, and cytb). We observed slight differences in the overall body size between females of both populations, and two well-defined geographical genetic lineages. This suggests the existence of two cryptic subspecies within S. t. tripectinata corresponding to two different island groups. Furthermore, we bring to light the necessity to provide new and updated morphological, molecular, and phylogenetic data to clarify the taxonomic status of S. tripectinata.

The diversity and biology of Cryptosporidium that is specific for rats (Rattus spp.) are not well studied. We examined the occurrence and genetic diversity of Cryptosporidium spp. in wild brown rats (Rattus norvegicus) by microscopy and polymerase chain reaction (PCR)/sequencing targeting the small subunit rDNA (SSU), actin and HSP70 genes. Out of 343 faecal samples tested, none were positive by microscopy and 55 were positive by PCR. Sequence analysis of SSU gene revealed the presence of Cryptosporidium muris (n = 4), C. andersoni (n = 3), C. ryanae (n = 1), C. occultus (n = 3), Cryptosporidium rat genotype I (n = 23), Cryptosporidium rat genotype IV (n = 16) and novel Cryptosporidium rat genotype V (n = 5). Spherical oocysts of Cryptosporidium rat genotype I obtained from naturally-infected rats, measuring 4.4–5.4 μm × 4.3–5.1 μm, were infectious to the laboratory rats, but not to the BALB/c mice (Mus musculus) nor Mongolian gerbils (Meriones unguiculatus). The prepatent period was 3 days post infection and the patent period was longer than 30 days. Naturally- and experimentally-infected rats showed no clinical signs of disease. Percentage of nucleotide similarities at the SSU, actin, HSP70 loci between C. ratti n. sp. and the rat derived C. occultus and Cryptosporidium rat genotype II, III, IV, and V ranged from 91.0 to 98.1%. These genetic variations were similar or greater than that observed between closely related species, i.e. C. parvum and C. erinacei (93.2–99.5%). Our morphological, genetic and biological data support the establishment of Cryptosporidium rat genotype I as a new species, Cryptosporidium ratti n. sp.

Although there is evidence that interspecific hybridization can initiate invasion by nonnative plants, there are few documented examples of novel hybridization events between introduced plant species already exhibiting invasive behavior. We conducted morphometric and molecular analyses of toadflax plants with intermediate morphology found at two sites in Montana, which were co-invaded by yellow toadflax and Dalmatian toadflax. Field-collected putative hybrid plants had intermediate morphometric scores (mean 0.47, on a scale of 0.0 = indistinguishable from Dalmatian toadflax to 1.0 = indistinguishable from yellow toadflax) for a suite of phenotypic traits that differentiate the parent species (leaf length : width ratio, growth form, seed morphology, inflorescence type, and ventral petal shape). Inter-simple sequence repeat (ISSR) analysis of a subset of these putative hybrids revealed combinations of species-diagnostic bands, confirming the presence of DNA from both parent species. Controlled interspecific hand-pollinations generated viable first generation (F1) hybrid plants that also had intermediate morphometric scores (mean 0.46) and a mix of species-diagnostic ISSR bands from both parents. The hand-generated F1 hybrids crossed readily with both parent species to produce viable first generation backcrossed (BC1) plants. Our results confirm that hybridization is occurring between invasive populations of yellow toadflax and Dalmatian toadflax, and that the hybrid progeny are viable and fertile. This example of hybridization between alien congeners is of concern as the parent taxa are already known to be highly invasive. Further research is needed to assess the invasive potential of hybrid toadflax populations, and the likelihood of introgressive trait transfer between the parent species.

Pleistocene biogeography of conifer species is poorly known in much of western North America. We conducted morphological studies on 201 conifer cones and cone fragments recovered from Pleistocene sediments at the Ziegler Reservoir fossil site (2705 m) near Snowmass Village, Colorado. The basin, formed ~155–130 ka, contains fossil-bearing lacustrine, palustrine, and colluvial sediments spanning approximately 85 ka. Using a suite of morphological characters, particularly cone-scale bracts, we differentiated species of Abies, Picea, and Pseudotsuga. All fossil Abies specimens were assignable based on bract morphology to Abies concolor, which is currently absent from central Colorado (nearest populations are 160 km southwest of the site). A. concolor occurs only in sediments of MIS 5d and 5c. Pseudotsuga menziesii and Picea engelmannii cones occurred in sediments corresponding to MIS 5e, 5d, 5c, and 5a. A fourth conifer species, occurring in sediments of MIS 5e, 5d, 5c, and 5a, is difficult to assign to any extant species. Bract morphology is similar to Picea pungens, which grows near the site today, but scale morphology is unlike P. pungens. These fossils may represent ancestral P. pungens, an extinct variant, or an extinct sister species.

Wedge clam, Donax scortum occurrence was studied from February 2009 to January 2010 in Padukere sandy beach, Karnataka (India). The following biological parameters were measured in 2367 individuals of the wedge clam, Donax scortum: length, breadth, width, total weight, shell weight, wet meat weight, dry meat weight and number of clams. The calculated linear equation of length-breadth and length-width relationships were B = 3.5968 + 0.5421L and W = 0.8191 + 0.4345L respectively. The length-total weight, length-wet meat weight, length-dry meat weight and length-shell weight relationships were 0.0001414L3.0224, W = 0.0000037L3.5204, W = 0.0000011L3.4513 and 0.0000852L2.9912 respectively. The values of correlation coefficient for different shell dimensional relationships were very close to unity. It was clear from the study that the most ideal period for clam harvest was April, June and December, especially when condition index was maximum. Length showed a strong correlation with silt and clay. Dry meat weight was negatively correlated with air temperature. Shell weight showed positive correlation with length, breadth, width and dry meat weight, and abiotic parameters, silt and clay. Condition index was positively correlated with electrical conductivity, whereas it was negatively correlated with dissolved oxygen. It showed a strong positive correlation with salinity. Seasonal fluctuation of condition index in D. scortum was probably related to reproductive activity. The harvesting of wedge clam could be carried out considering the maximum sustainable yield without over-exploitation

The cosmopolitan and often ecologically dominant genus Terschellingia (Nematoda: Linhomoeidae), with 38 nominal species, is taxonomically a problematic taxon. Its species show high morphological plasticity, possess few diagnostic characters and identification keys are lacking. A revision of the genus was carried out based on morphological and morphometric data from the literature and from observations of specimens collected in Cienfuegos Bay, Caribbean Sea, Cuba. The diagnosis of the genus Terschellingia is emended. Of the current 38 nominal species, 15 are considered as valid species based on morphological characters related to size and position of amphidial fovea, presence/position of cephalic and cervical setae, presence/size/shape of pharyngeal bulb, shape of spicular apparatus and shape of tail. Tabular and pictorial keys were provided based on these characters. Three sympatric species: T. communis, T. gourbaultae and T. longicaudata were redescribed based on recently collected Cuban specimens. Each of them showed relatively large differences in body size in comparison with the respective type specimens, suggesting possible variation due to local environmental differences. The highest intraspecific variation pertains for the most widely spread cosmopolitan species T. longicaudata, suggesting that morphological plasticity enhanced adaptation to different environmental conditions. The notable taxonomic inflation within the genus (14 species inquirendae, 9 junior synonyms), probably also present in other highly specious genera of marine nematodes, can lead to an overestimation of the alpha-diversity.

Antheraea assamensis (Helfer, 1837) is a sericigenous insect with distinct coloration, namely green, blue, orange and yellow in their larval stages and these are multivoltine in culture. However, wild morphs with diapause are found in dense forests. In this study, the wing venation and its variation in A. assamensis were analysed using traditional as well as landmark-based morphometric methods. The venation patterns are reviewed and significant changes in veins recorded. The samples were analysed using geometric morphometric methods and other conventional statistical methods. Landmark-based data were measured among selected veins and the centroid sizes, procrustes to tangent distance of different veins were analysed. Results show significant differences in some of the veins among the intraspecies of A. assamensis. The results suggest that both visual comparison as well as a quantitative approach in A. assamensis may lead to unsound taxonomic conclusions at intraspecies level.

Morphometric analysis of magnetic resonance (MR) scans in 88 traumatic brain injury (TBI) patients demonstrated significantly larger ventricle-to-brain ratios (VBR) and temporal horn volumes, and significantly smaller fornix-to-brain ratios (FBR) and corpus callosum (CC) area measurements, compared to 73 controls. Additionally, TBI patients were grouped according to Glasgow Coma Scale (GCS) for a within-TBI sample comparison so that severity of injury on brain morphology could be examined. The severe TBI group (GCS = 3–6) differed from the mild and moderate injury groups on measures of the internal capsule, VBR, temporal horn volume, and CC. In a separate analysis wherein the TBI subjects were grouped by degree of fornix atrophy, the group with the smallest fornix size demonstrated the lowest memory performance. Furthermore, anatomic measures correlated with severity of injury, and tests of memory and motor function. Results demonstrate the diffuse nature of degeneration in TBI with more severe injury, and that quantified MR identified morphologic changes relate to neuropsychological outcome. (JINS, 1995, 1, 17–28.)

We studied 11 patients with pulmonary atresia and intact ventricular septum who died at ages ranging from one day to three years. All but two neonates died after surgery. Pre-surgical catheterization with left and right cineventriculography was performed in nine patients, and quantitative parameters were calculated. By histologic examination of transverse transmural sections, the incidence and extent of myocardial ischemia or infarction were determined, as well as the percentage area per field occupied by vessels, myocytes orientated longitudinally or transversely, interstitial space, and disarrayed fibers. Comparable sections were obtained from six normal neonates to constitute a control group. Myocardial ischemia or infarction were frequent complications. Morphometric analysis demonstrated a significant difference between the control group and the patients with regard to the percentage of myocytes (longitudinal and transverse), the percentage of area occupied by myocardial disarray, and the presence of fibrosis. The highest values for the proportion of disarray were seen in those patients with small right ventricles. The percentage of myocytes varied, but the highest degree of disarray always correlated with the lowest percentage of myocytes. The highest percentage value of left ventricular disarray was associated with the lowest ratio of left ventricular mass to end diastolic volume (inadequate hypertrophy). The lowest indices of contractility and left ventricular pump function were associated with myocytes less than 55% of normal. A linear correlation was found between the percentage area of fibrosis and the ratio of systolic pressure to end systolic volume in the left ventricle, arid between fibrosis and ejection fraction. Values for fibrosis exceeding 15% were associated with decreased functional indices. Disarray, therefore, seems to express a primary inability of myocardium to respond to anomalous overload with adequate hypertrophy and/or hyperplasia.

Specific procedures are described to measure the body dimensions of large African predators; these include measurements of body and tail length, head, canines, mane, limbs, paws and testes. Data on body mass and dimensions were collected from an immobilized sub-adult male African lion Panthera leo. He was 40 months old and weighed 190 kg. After being immobilized, data on body mass and dimensions were collected by a team comprising a scribe, two operators and assistants. The procedures of measuring the 43 variables on body dimensions were then repeated three times in quick succession, each session lasting about 10 min. The four sessions were handled as if a different individual was measured each time. Except for reports on body mass of lions, there is a paucity of comparable data in the literature for most of the variables presented in this paper. The small dispersion measures of the 43 variables suggest that the specific procedures to measure the body dimensions of a large African predator such as the lion have a high degree of repeatability and can be applied with confidence. The procedure has been used to obtain data on body dimensions of a large number of lions as well as dead specimens of black-backed jackal Canis mesomelas, caracal Caracal caracal and Cape fox Vulpes chama. The data form part of a database on body dimensions of large African predators created by ALPRU. The objective is to develop non-invasive techniques to determine whether wild predator populations might have been subjected to abnormal growth and development as a result of negative impacts on their habitat and thus food base.

Five populations of habitat-specific, morphologically distinct Algophagus mites (Astigmata: Algophagidae) were found to occur on the sub-Antarctic Prince Edward islands. Although these mites did not differ among themselves with respect to idiosomal setal characteristics, which were also similar to those of known sub-Antarctic Algophagus taxa, morphometric analyses revealed leg and idiosomal size differences. In addition to morphometric differences, leg setal positions and porose axillary organ structure, indicated that the morphotypes were taxonomically discrete. The taxa were found to include the known A. antarcticus Hughes, 1955, A. laticollaris Fain, 1974 (new status), a sub-species of A. semicollaris Fain, 1974, and two new species, A. brachytarsus sp. nov. and A. macrolithus sp. nov. With the exception of A. semicollaris, the taxa are described or redescribed in this paper. Morphological features of the females in particular, and notably leg proportions, claw dimensions, and apodeme positions, corroborate discrete terrestrial (A. brachytarsus and A. macrolithus) and aquatic forms (A. antarcticus, A. laticollaris, and A. semicollaris), and vary in a manner suggesting a functional relationship to habitat occupation by the various taxa.

Magnetic resonance (MR) images and neuropsychological testing data of 69 carbon monoxide (CO) poisoned patients were prospectively obtained within 1 day of CO poisoning, two weeks and six months. CO patients' Day 1 cross-sectional fornix surface area measurements, corrected for head size by using a fornix-to-brain ratio (FBR), were compared to normal age and gender-matched controls. Additionally, a within-subjects analysis was performed comparing the mean areas between CO patients' Day 1, 2 weeks and 6-month FBR. The FBR was correlated with patients' neuropsychological data. There were no significant differences between CO patients' Day 1 fornix measurements compared to normal control subjects. However, significant atrophic changes in the fornix of CO poisoned patients occurred at two weeks with no progressive atrophy at 6 months. By 6 months, CO patients showed significant decline on tests of verbal memory (when practice effects were taken into account), whereas visual memory, processing speed and attention/concentration did not decline. This study indicates that CO results in brain damage and cognitive impairments in the absence of lesions and other neuroanatomic markers. (JINS, 2001, 7, 640–646.)