Germinated seeds of wild oat populations that were susceptible (S) or resistant (R) to triallate at the recommended soil-applied rate (1.7 kg/ha) were treated with six triallate concentrations on filter paper in petri dishes. Measurement of shoot length 8 d after treatment provided an accurate indication of differences among populations, and was more reliable than determining shoot fresh weight. ED50 values (herbicide concentrations that reduced shoot length by 50% relative to untreated controls), derived from nonlinear regression analysis, indicated four and five levels of response to triallate among eight S and seven R populations, respectively. The ED50 values varied from 0.11 to 11 ppm a.i. triallate for the most susceptible to the most resistant populations, respectively. Routine testing of wild oat samples suspected of resistance, at triallate concentrations of 0.5 or 1 ppm in the petri dish bioassay, effectively identified populations that had become resistant to the recommended soil-applied rate.

A bioassay using red beet root length indicated an increase in imazethapyr bioactivity in Berryland sand (BLS) as soil pH increased from 3.7 to 6.5. Increasing pH above 6.5 had no effect on imazethapyr bioactivity. The lowest imazethapyr concentrations detected by the bioassay at pH 6.5 were 0.5, 1, 2.5, and 5 to 10 μg kg−1 in acidwashed quartz sand, BLS, Aura loamy sand, and muck soil, respectively. After application of 0.07 kg ae ha−1 with 12.5 mm of simulated rainfall at 3-d intervals, imazethapyr remained in the 0 to 15 cm soil in BLS soil columns. Under this rainfall regime, the herbicide moved 30 cm deep 5 mo after application, but residues decreased significantly in surface soil. Imazethapyr bioactivity was highest and mobility was lowest at low soil temperature (10 C) and low moisture (4 to 6% w/w). Imazethapyr movement was monitored successfully using the red beet root length bioassay, and an herbicide residue map was developed to show the pattern of imazethapyr bioactivity in soil columns.