Introduction. The aim of ourstudy was to analyze the effect of various levels of light availabilityin the fruit microenvironment (induced by summer pruning) on somefruit quality traits. Materials and methods. Two levelsof summer pruning were set: removal of either 30% or 60% of thecanopy leaf area. These treatments were compared with the control(unpruned vines). Two canopy layers (upper and lower) were identifiedin each treatment and in the control vines. At harvest, yield percane and per shoot was recorded in the two canopy layers; fruitfresh weight, flesh firmness, total soluble solid content and totaltitratable acidity were measured. Fruit Total Antioxidant Capacity (TAC)and Total Polyphenols (TPH) were determined at kiwifruit harvestand post-harvest (60 days after storage at 1 °C) in relation totheir position within the canopy (layer) and to the pruning treatmentapplied. Results. Removing 60% of the canopy leaf areainduced higher titratable acidity and flesh firmness of kiwifruitsat harvest; it reduced fruit weight and crop yield, but increasedTAC and TPH compared with kiwifruits of control. Removing 30% leaf areadid not reduce mean fruit weight and crop yield; it increased TACand TPH at harvest by 20%. Post-harvest TAC and TPH evolution didnot differ among treatments. Discussion and conclusion.Pergola-trained kiwifruit vines showed good performances in termsof crop yield and fruit quality when summer pruning reduced the LeafArea Index (LAI) to 3. More intense pruning resulted in a significantincrease in fruit TAC, but reduced fruit weight and crop yield,and delayed softening of pulp.